Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study

Abstract

PurposeOsteoarthritis (OA) is a degenerative disease characterized by a progressive loss of articular cartilage extracellular matrix and is due to functional impairments occurring in chondrocytes. In previous works, we highlighted that Regenerative Tissue Optimization (TO-RGN) treatment with radioelectric asymmetric conveyer (REAC) technology influenced the gene expression profiles controlling stem cell differentiation and the pluripotency of human skin-derived fibroblasts in vitro. Since interleukin-1 beta signaling has been implicated in the induction and progression of this disease (through metalloproteinase-3 synthesis and nitric oxide production), we investigated whether REAC TO-RGN might influence the biochemical and morphological changes induced by interleukin-1 beta in normal and OA chondrocytes.MethodsThe induction of metalloproteinase-3 and proteoglycan synthesis was evaluated by a solid-phase enzyme-amplified sensitivity immunoassay, and nitric oxide production was evaluated with the Griess method. Ultrastructural features were observed by transmission electron microscopy.ResultsREAC TO-RGN treatment decreased nitric oxide and metalloproteinase-3 production in normal and OA chondrocytes, while inducing an increase in proteoglycan synthesis. OA chondrocytes were more affected by REAC TO-RGN treatment than were normal chondrocytes. Ultrastructural changes confirmed that REAC TO-RGN may counteract the negative effects of interleukin-1 beta incubation.ConclusionThe results of this in vitro study suggest that REAC TO-RGN treatment may represent a new, promising approach for the management of OA.

Changes in Ultrastructure and Cytoskeletal Aspects of Human Normal and Osteoarthritic Chondrocytes Exposed to Interleukin-1β and Cyclical Hydrostatic Pressure

“…Mitochondria and Golgi bodies were counted and expressed the mean ± standard deviation (SD) of triplicate values for each experiment. The presence of cytoplasmic vacuolization was reported as percentages of the cells with a number ≥ of five vacuoles, considered as point of reference [ 59 ]. χ 2 test was used to analyze the percentage of vacuolization and the percentage of cells with marginated chromatin [ 24 , 25 ].…”

Neurological morphofunctional differentiation induced by REAC technology in PC12. A neuro protective model for Parkinson’s disease

“…REAC tissue optimization (TO) regenerative treatments (RGN) includes a set of treatment protocols 1 2 3 4 5 20 which are carried out by the ACP immersed in the medium of the cell culture or covering the treated area with ACP. The REAC TO-RGN has been administered 168 hours without interruption.…”

Stem cell senescence. Effects of REAC technology on telomerase-independent and telomerase-dependent pathways

“…Furthermore, REAC TO-RGN exposure proved effective in antagonizing the aging process in vitro 333435. Here, we show that REAC TO-RGN exposure also affected telomerase-dependent senescence mechanisms, by transcriptionally enhancing the expression of TERT, and counteracting telomere shortening.…”