Effects of red and near-infrared LED light therapy on full-thickness skin graft in rats

Martignago, Cintia Cristina Santi; Tim, Carla Roberta; Assis, Lívia; Silva, Viviane Ribeiro da; Santos, Estefany Camila Bonfim Dos; Vieira, Fabiana Nascimento; Parizotto, Nivaldo Antônio; Liebano, Richard Eloin

doi:10.1007/s10103-019-02812-6

Cited by 32 publications

(20 citation statements)

References 31 publications

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“…Tani et al [50] irradiated human osteoblasts and mesenchymal stromal cells with 0.4 J/cm 2 using either red (635 nm) or NIR (808 nm) and found that red was more effective in promoting bone regeneration while NIR modified the cytoskeleton assembly and mineralization pattern. Using a full thickness skin graft rat model, Martignago et al [51] demonstrated that red LED (630 nm) was more efficient in tissue repair as assessed by skin graft score, level of transforming growth factor β protein expression, and density of collagen fibers compared with NIR (850 nm). Keszler et al [52] demonstrated that irradiating ex vivo murine facial arteries resulted in much more vasodilatation when using red (670 nm) compared with NIR LED (830 nm) irradiation (10 mW/cm 2 , 6 J/cm 2 ).…”

Section: Discussionmentioning

confidence: 99%

Microcirculatory Response to Photobiomodulation—Why Some Respond and Others Do Not: A Randomized Controlled Study

et al. 2020

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Conclusions: We demonstrated that PBM induces arteriolar vasodilatation that results in both immediate and long-lasting increased capillary flow and tissue perfusion in healthy individuals. This response was wavelengthdependent and modified by skin temperature. These findings regarding physiological parameters associated with sensitivity or resistance to PBM provide information of direct relevance for patient-specific therapy. Lasers Surg. Med.

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Section: Discussionmentioning

confidence: 99%

Microcirculatory Response to Photobiomodulation—Why Some Respond and Others Do Not: A Randomized Controlled Study

et al. 2020

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“…The photons emitted by LED irradiation are absorbed by specific cellular photoreceptors, initiating several intracellular reactions, which lead to changes in membrane potential, O 2 consumption and RNA and DNA synthesis (Prindeze et al, 2012;Avci et al, 2013). These events contribute to the therapeutic effects of LED irradiation demonstrated in the repair of some tissues, such as muscle, skin, tendon, and periodontium (de Melo et al, 2016;Chen et al, 2019;Martignago et al, 2019;Nascimento et al, 2019). Despite benefiting tissue repair, the therapeutic potential of LED irradiation is still controversial due to the divergence of protocols used (Yeh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of red LED irradiation on fibroblasts and co-culture of adipose-derived mesenchymal stem cells

Theodoro

Fujii

Lucke

et al. 2020

Heliyon

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The objective of this study was to evaluate the effects of red Light Emiting Diode (red LED) irradiation on fibroblasts in adipose-derived mesenchymal stem cells (ASC) co-culture on the scratch assay. We hypothesized that red LED irradiation could stimulate paracrine secretion of ASC, contributing to the activation of genes and molecules involved in cell migration and tissue repair. ASC were co-cultured with NIH/3T3 fibroblasts through direct contact and subjected to red LED irradiation (1.45 J/cm 2 /5min6s) after the scratch assay, during 4 days. Four groups were established: fibroblasts (F), fibroblasts þ LED (FL), fibroblasts þ ASC (FC) and fibroblasts þ LED þ ASC (FLC). The analyzes were based on Ctgf and Reck expression, quantification of collagen types I and III, tenomodulin, VEGF, TGF-β1, MMP-2 and MMP-9, as well as viability analysis and cell migration. Higher Ctgf expression was observed in FC compared to F. Group FC presented higher amount of tenomodulin and VEGF in relation to the other groups. In the cell migration analysis, a higher number of cells was observed in the scratched area of the FC group on the 4 th day. There were no differences between groups considering cell viability, Reck expression, amount of collagen types I and III, MMP-2 and TGF-β1, whereas TGF-β1 was not detected in the FC group and the MMP-9 in none of the groups. Our hypothesis was not supported by the results because the red LED irradiation decreased the healing response of ASC. An inhibitory effect of the LED irradiation associated with ASC co-culture was observed with reduction of the amount of TGF-β1, VEGF and tenomodulin, possibly involved in the reduced cell migration. In turn, the ASC alone seem to have modulated fibroblast behavior by increasing Ctgf, VEGF and tenomodulin, leading to greater cell migration. In conclusion, red LED and ASC therapy can have independent effects on fibroblast wound healing, but the combination of both does not have a synergistic effect. Therefore, future studies with other parameters of red LED associated with ASC should be tested aiming clinical application for tissue repair.

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“…Martignago and collaborators Martignago et al (2020) carried out a study in which skin grafts were irradiated daily for ten consecutive days, starting immediately after the surgery, using a red (630 nm) or near infrared (850 nm) LED. The results showed that the red wavelength LED signi icantly enhanced the skin graft score in relation to the NIR group and increased the expression of TGFβ and the density of collagen ibers compared with the other experimental groups.…”

Section: Figure 5 Differences In the Expression Of Tgf-β1 Between Led And Control Groupsmentioning

confidence: 99%

Influence of 627 Nm Wavelength Light Emitting Diode Phototherapy on Secondary Intention Wound Healing

Suckow

Bini

Hosni

et al. 2021

Int. J. Res. Granthaalayah

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Background: The use of photobiomodulation can be effective in the healing of tissues, highlighting the use of LED in tissue repair. Was evaluated the effect of 627 nm wavelength LED irradiation on VEGF-α and TGF-β1 expressions in a model of secondary intention wound healing. Methods: The evaluations were performed using a dorsal puncture model and the sample consisted of 120 Wistar rats, randomly divided into LED groups submitted to 3, 7, 14 and 21 irradiation treatment for 100 s, 7 J / cm2, and control groups. Histological analysis, immunohistochemistry and contraction of the lesion were performed. Results: LED irradiation led to effective wound regeneration results. Similar effects were obtained with vascular, exudative, and proliferative phenomena. VEGF and TGF-β1 levels were increased in LED-treated groups with initial treatments. Collagen analysis revealed that LED-treated groups levels were increased on days 14- and 21 of the treatments. An acceleration in tissue regeneration was observed in LED treated groups. Macroscopically visible results showing a complete healing process was evident after 14 days of treatment. Conclusion: 627 nm LED irradiation effectively accelerated wound healing leading complete closure of the skin in a shorter time compared to control group.

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Effects of red and near-infrared LED light therapy on full-thickness skin graft in rats

Cited by 32 publications

References 31 publications

Microcirculatory Response to Photobiomodulation—Why Some Respond and Others Do Not: A Randomized Controlled Study

Microcirculatory Response to Photobiomodulation—Why Some Respond and Others Do Not: A Randomized Controlled Study

Inhibitory effect of red LED irradiation on fibroblasts and co-culture of adipose-derived mesenchymal stem cells

Influence of 627 Nm Wavelength Light Emitting Diode Phototherapy on Secondary Intention Wound Healing

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