Effects of reaction temperatures and reactant concentrations on the antimicrobial characteristics of copper precipitates synthesized using L-ascorbic acid as reducing agent

Ramos, Ana Rose; Tapia, Alvin Karlo G.; Piñol, Chrysline Margus N.; Lantican, Nacita B.; Mundo, Ma. Lourdes F. Del; Manalo, Ronniel D.; Herrera, Marvin U.

doi:10.1016/j.jsamd.2018.12.009

Cited by 11 publications

(8 citation statements)

References 24 publications

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“…As the temperature increased e.g., at 50 °C, vitamin C showed smaller zones of inhibition against all bacterial strains which showed that antibacterial activity of vitamin C is maximum at low to moderate temperatures. Other researchers have evaluated the same results; they also found that vitamin C showed maximum antibacterial activity at 25 °C, as compared to 85°C against S. aureus and E. coli (Ramos et al, 2019). Our results are also consistent with Ranjan et al (2012), they stated the antimicrobial action of cinnamon extract as well as aqueous garlic against E. faecalis, S. aureus, E. Coli and Proteus mirabilis at 40 °C, 60 °C 80 °C, 100°C and 120 °C.…”

Section: Discussionsupporting

confidence: 91%

“…Three autoclaved Eppendorf tubes were taken and (20 mg/ml) of vitamin C solution was added in each tube to evaluate the stability at various temperatures (4°C, 37°C, and 50°C ). These three Eppendorf tubes were kept at (4°C, 37°C, and 50°C) for 36 h (Aramwit et al, 2010;Ramos et al, 2019). The antimicrobial action of these samples was assessed after the incubation period, by using the agar well diffusion method against Gram-negative bacteria (K. pneumoniae, E. coli, P. aeruginosa and P. mirabilis and Gram-positive bacteria (B. subtilis, B. licheniformis, and S. aureus).…”

Section: Stability Of Vitamin C At Different Temperatures and Phmentioning

confidence: 99%

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Evaluation of antibacterial activity of vitamin C against human bacterial pathogens

et al. 2023

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Now a day’s multidrug resistance phenomenon has become the main cause for concern and there has been an inadequate achievement in the development of novel antibiotics to treat the bacterial infections. Therefore, there is an unmet need to search for novel adjuvant. Vitamin C is one such promising adjuvant. The present study was aimed to elucidate the antibacterial effect of vitamin C at various temperatures (4°C, 37°C and 50°C) and pH (3, 8, and 11), against Gram-positive and Gram-negative bacteria at various concentrations (5-20 mg/ml) through agar well diffusion method. Growth inhibition of all bacterial strains by vitamin C was concentration-dependent. Vitamin C significantly inhibited the growth of Gram-positive bacteria: Bacillus licheniformis (25.3 ± 0.9 mm), Staphylococcus aureus (22.0 ± 0.6 mm), Bacillus subtilis (19.3 ± 0.3 mm) and Gram-negative bacteria: Proteus mirabilis (27.67 ± 0.882 mm), Klebsiella pneumoniae (21.33±0.9 mm), Pseudomonas aeruginosa (18.0 ± 1.5 mm) and Escherichia coli (18.3 ± 0.3 mm). The stability of vitamin C was observed at various pH values and various temperatures. Vitamin C showed significant antibacterial activity at acidic pH against all bacterial strains. Vitamin C remained the stable at different temperatures. It was concluded that vitamin C is an effective and safe antibacterial agent that can be used in the future as an adjunct treatment option to combat infections in humans.

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Section: Discussionsupporting

confidence: 91%

Section: Stability Of Vitamin C At Different Temperatures and Phmentioning

confidence: 99%

Evaluation of antibacterial activity of vitamin C against human bacterial pathogens

et al. 2023

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“…The energy required to trigger copper ions reduction into copper particles declines with the temperature. Additionally, at higher temperatures, the probability of one molecule colliding and reacting with another increases [15]. In this study, the increase of temperature to 70 °C allowed having 63.6% of ions copper reduction efficiency after just 20 min of reaction confirming what was found in the literature [15].…”

Section: Fig 5 Copper Ions Reduction Efficiency In Function Of Bain-marie Temperaturesupporting

confidence: 88%

“…Additionally, at higher temperatures, the probability of one molecule colliding and reacting with another increases [15]. In this study, the increase of temperature to 70 °C allowed having 63.6% of ions copper reduction efficiency after just 20 min of reaction confirming what was found in the literature [15]. However, under this temperature, water started to evaporate which leads to a decrease in treated water quantity and eventually the studied concentration of ascorbic acid as a reducing agent will be different from its initial value.…”

Section: Fig 5 Copper Ions Reduction Efficiency In Function Of Bain-marie Temperaturementioning

confidence: 99%

Synthesis of copper particles and elimination of cupric ions by chemical reduction

Mahfouf

Djerad

Bouchareb

2020

Environmental Research and Technology

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Development of enhanced methods for copper particles synthesis is crucial for the improvement of material science and technology. Therefore, in this study a successful synthesis of copper metal was achieved by chemical reduction. Ascorbic acid was used as a reducing agent. In the presence of soda, copper sulphate pentahydrated (CuSO4, 5H2O) with acid ascorbic at 60 °C of temperature produced metallic copper powder with the total degradation (100%) of copper ions (Cu 2+ ). The presence of hydroxide ions (OH -) is necessary to achieve and improve the chemical reduction reaction. Several parameters, as reducing agent volume, reaction temperature and soda quantity were investigated and checked their impact in this research study. The obtained powder was washed and dried in the fresh air then analysed by X-ray diffraction.

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“…A series of methods of Cu nano-powders obtaining involves reduction reactions of copper (II) salts with diverse reducing agents such as ascorbic acid [ 10 , 11 ], hydrazine [ 12 ], hypophosphite [ 13 ], sodium borohydride [ 14 , 15 , 16 ], hydrogen [ 17 ], and sodium phosphinate monohydrate (NaH 2 ·PO 2 ·H 2 O) in diethylene, which has the role of both reaction environment and reducing agent [ 18 ], hydrazine borane (BH 4 N 2 ) [ 19 ], or ascorbic acid and sodium borohydride [ 10 ]. Copper compounds used as precursors for metallic copper or other copper derivatives are copper chloride [ 20 , 21 ], copper nitrate (Cu(NO 3 ) 2 × 3H 2 O) [ 22 , 23 ], copper sulphate (CuSO 4 × 5H 2 O) [ 20 , 21 , 24 , 25 ], or copper acetate (Cu(CH 3 COO) 2 × H 2 O) [ 20 , 21 , 22 ]. Copper is used in many applications due to its strong antimicrobial properties and due to its economic advantages being less expensive than gold and silver [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Poly (Lactic Acid)/Copper Nanocomposites for Food Packaging Materials

et al. 2023

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Composites based on polylactic acid (PLA) and copper for food packaging applications were obtained. Copper clusters were synthesized in polyethylene glycols 400 and 600, respectively, using ascorbic acid as a reducing agent, by reactive milling. Copper clusters were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FT-IR), and Ultraviolet-Visible (UV-VIS) spectroscopy. Copper/PLA composites containing Proviplast as plasticizer were characterized by FT-IR spectroscopy, mechanical tests, Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA), absorption of the saline solution, contact angle, and antibacterial properties. It was observed that the concentration of Copper/PEG influenced the investigated properties. The mechanical properties of the samples decreased with the increasing of Copper/PEG concentration. We recorded the phase transformation temperatures and identified the exothermic or endothermic processes. The lowest absorption values were recorded in the case of the sample containing 1% Cu. The contact angle decreases with the increase in the concentration of the PEG 600-Cu mixture in the recipes. The increase in the content of Cu clusters favors the decrease in the temperature, taking place 15% wt mass losses. The obtained composites showed antibacterial properties for all tested strains. These materials could be used as alternative materials for obtaining biodegradable food packaging.

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Effects of reaction temperatures and reactant concentrations on the antimicrobial characteristics of copper precipitates synthesized using L-ascorbic acid as reducing agent

Cited by 11 publications

References 24 publications

Evaluation of antibacterial activity of vitamin C against human bacterial pathogens

Evaluation of antibacterial activity of vitamin C against human bacterial pathogens

Synthesis of copper particles and elimination of cupric ions by chemical reduction

Antimicrobial Poly (Lactic Acid)/Copper Nanocomposites for Food Packaging Materials

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