Effects of Radix Astragali and Its Split Components on Gene Expression Profiles Related to Water Metabolism in Rats with the Dampness Stagnancy due to Spleen Deficiency Syndrome

Zhao, Wenxiao; Cui, Ning; Jiang, Haiqiang; Ji, Xuming; Han, Xiao; Han, Baohui; Wang, Tong; Wang, Shijun

doi:10.1155/2017/4946031

Cited by 15 publications

(14 citation statements)

References 25 publications

(29 reference statements)

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“…Chinese medicine practice over thousands of years has confirmed that A. membranaceus functions to replenish qi, which strengthens body resistance, promotes diuretic detoxification, heals sores, and prompts muscles. 9 – 12 It contains many chemical components, such as polysaccharides, saponins, flavonoids, amino acids, and certain trace elements. Our study found that Chahuangjing exhibits diuretic, tritium-removing, and free-radical-scavenging effects, which reduce internal HTO radiation injury.…”

Section: Discussionmentioning

confidence: 99%

Effects of Chahuangjing on Decorporation and Radiation Protection Against Tritiated Water

Zuo

Qiu

Houwen³

et al. 2018

Dose-Response

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The purpose of this study was to investigate the effects of Chahuangjing, a novel traditional Chinese medicinal compound, on decorporation and radiation protection against tritiated water (HTO). Sixty male specific-pathogen-free-grade C57BL/6J mice were randomly divided into 12 groups: mice in 4 control groups were intraperitoneally injected with sterile water; mice in 4 HTO groups were intraperitoneally injected with 11.1 × 105 Bq/g of HTO; and mice in the other 4 groups were administered with HTO and a Chahuangjing compound (0.2 mL, once daily). After 1, 7, 14, and 21 days, the mice were killed and samples were collected. A liquid scintillation counting method was used for tritium measurement. A fully automated hematology analyzer was used to assess blood samples. The superoxide dismutase (SOD) and malondialdehyde (MDA) content was analyzed using commercial kits. Chahuangjing significantly increased decorporation and shortened the effective half-life of tritium. To a certain extent, Chahuangjing alleviated the HTO-induced reduction in white blood cells and elevated red blood cells after HTO exposure. Moreover, Chahuangjing alleviated the HTO-induced reduction in SOD activity and reduced MDA. Our study demonstrated that Chahuangjing can enhance the elimination of tritium and reduce free radicals to alleviate HTO-induced radiation injury.

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Section: Discussionmentioning

confidence: 99%

Effects of Chahuangjing on Decorporation and Radiation Protection Against Tritiated Water

Zuo

Qiu

Houwen³

et al. 2018

Dose-Response

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“…Based on the equivalent dose coefficient algorithm, the rat equivalent dose was 6.3 times that of adults per body weight unit. The abstract was administered by gavage with the dose of 5.40g·kg −1 ·d −1 , 0.54g/ml, 10ml/kg [ 21 ]. The administration was performed once a day, seven consecutive days [ 6 ].…”

Section: Methodsmentioning

confidence: 99%

The Effects of Radix Astragali Water Abstract on Energy Metabolism in Rat Yang-Deficiency Cold Syndrome Model through PPAR Signaling Pathway

Zhao

Zong

et al. 2018

Evidence-Based Complementary and Alternative Medicine

Self Cite

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Radix Astragali (RA) herb with warm property and significant “tonifying qi” effects is indicated for the syndrome of internal cold due to Yang deficiency. The purpose of this research was to explore effects of Radix Astragali (RA) through PPAR signaling pathway on gene expression profiles related to energy metabolism in rats with the Yang-deficiency cold (YDC) syndrome, for identifying the pathological mechanism of Yang-deficiency cold (YDC) syndrome and the effects mechanism of RA. The results indicated that RA could significantly increase body weight (BM), cold and heat tendency (CT), overall temperature (OT), rectum temperature (RT), toe temperature (TT), energy intake (EI), and V(O2)/V(CO2) ratio (which indicates basal metabolism, BM) (P<0.05), enhancing the depressed metabolic function in YDC syndrome model rat. Our data also indicated differentially expressed genes (DEGs) related to energy metabolism involving lipids, carbohydrates, and amino acids metabolic process; the expression of CPT-1 and FABP4 (ap2) was improved; PPAR, Glycolysis, Wnt, cAMP, MAPK, AMPK, and fatty acid degradation signaling pathway may be related to energy metabolism. However, the Chinese herbal medicine RA plays a certain role in promoting the metabolism of substances and energy in rats by its warming and beneficial effect. Our results suggest that the mechanism underlying the function of RA may take effect through the regulation of PPAR signaling pathway and related gene expression. Lipids, carbohydrates, and amino acids metabolic process may be affected to adjust the reduced metabolic function in the model animals. In general, results indicate that RA could promote energy metabolism in rats with the YDC syndrome via PPAR signaling pathway regulating the expression of CPT-1 and FABP4 (ap2), which reflected the warm and qi tonifying properties of RA.

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“…Aer 3 d of continuous gavage, the drug was administered aer 30 min for the last gavage. [18][19][20] These model rats were randomly divided into: the model group (distilled water 20 mL kg À1 ); the furosemide positive control group (20 mg kg À1 ) and salted Alisma orientale groups including Fujian, Sichuan, Jiangxi, Hebei, Hubei, Guangxi, and Henan (the extract of the high dose was equivalent to 4.0 g kg À1 of the crude drug and the low dose was equivalent to 0.9 g kg À1 relative to the crude drug). All animal procedures were performed in accordance with the guidelines approved by the Experimental Animal Research Committee of China Pharmaceutical University and approved by the Animal Ethics Committee of China Pharmaceutical University.…”

Section: Animal Experimentsmentioning

confidence: 99%

The structural composition of components contributes to the superiority of the geoherb Alisma orientale for “diuresis and diffusing dampness”

Chen

et al. 2020

RSC Adv.

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Effects of Radix Astragali and Its Split Components on Gene Expression Profiles Related to Water Metabolism in Rats with the Dampness Stagnancy due to Spleen Deficiency Syndrome

Cited by 15 publications

References 25 publications

Effects of Chahuangjing on Decorporation and Radiation Protection Against Tritiated Water

Effects of Chahuangjing on Decorporation and Radiation Protection Against Tritiated Water

The Effects of Radix Astragali Water Abstract on Energy Metabolism in Rat Yang-Deficiency Cold Syndrome Model through PPAR Signaling Pathway

The structural composition of components contributes to the superiority of the geoherb Alisma orientale for “diuresis and diffusing dampness”

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