Effects of radio-frequency fields on bacterial cell membranes and nematode temperature-sensitive mutants

Güven, Reyhan Gül; Güven, Kemal; Dawe, Adam; Worthington, John J.; Harvell, Christopher; Popple, Amy L.; Smith, Tim; Smith, Brette; Pomerai, David I. de

doi:10.1016/j.enzmictec.2005.12.017

Cited by 12 publications

(6 citation statements)

References 24 publications

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“…Using a modified TEM exposure cell, which significantly reduces the temperature differential (to 0.1 8C), this heat-shock response was no longer detectable . A similar thermal explanation may also account for the apparent effect of RF fields on mutant phenotype prevalence in temperature-sensitive C. elegans mutants grown at intermediate temperatures [Gul-Guven et al, 2006]. This is in agreement with biophysical arguments that set limits on possible non-thermal interactions between RF fields and biological systems in equilibrium [Adair, 2003], and with the lack of any generally agreed mechanism for such non-thermal interactions.…”

Section: Introductionsupporting

confidence: 80%

Continuous wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16‐1 heat‐shock gene expression in Caenorhabditis elegans

Dawe¹,

Nylund²,

Leszczyński³

et al. 2007

Bioelectromagnetics

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Recent data suggest that there might be a subtle thermal explanation for the apparent induction by radiofrequency (RF) radiation of transgene expression from a small heat‐shock protein (hsp16‐1) promoter in the nematode, Caenorhabditis elegans. The RF fields used in the C. elegans study were much weaker (SAR 5–40 mW kg−1) than those routinely tested in many other published studies (SAR ∼2 W kg−1). To resolve this disparity, we have exposed the same transgenic hsp16‐1::lacZ strain of C. elegans (PC72) to higher intensity RF fields (1.8 GHz; SAR ∼1.8 W kg−1). For both continuous wave (CW) and Talk‐pulsed RF exposures (2.5 h at 25 °C), there was no indication that RF exposure could induce reporter expression above sham control levels. Thus, at much higher induced RF field strength (close to the maximum permitted exposure from a mobile telephone handset), this particular nematode heat‐shock gene is not up‐regulated. However, under conditions where background reporter expression was moderately elevated in the sham controls (perhaps as a result of some unknown co‐stressor), we found some evidence that reporter expression may be reduced by ∼15% following exposure to either Talk‐pulsed or CW RF fields. Bioelectromagnetics 29:92–99, 2008. © 2007 Wiley‐Liss, Inc.

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Section: Introductionsupporting

confidence: 80%

Continuous wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16‐1 heat‐shock gene expression in Caenorhabditis elegans

Dawe¹,

Nylund²,

Leszczyński³

et al. 2007

Bioelectromagnetics

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“…According to egg production rate differences between exposed and sham exposed groups, there is a significant decrease in the egg production (Figure 8-B). The reason for the decrease may be RF-induced stress [38] or nonthermal RF induced heat-shock [35,39,40] and this is an important area to investigate. Because, being affected by high frequency RFR of C. elegans egg laying rate as a member of nutrient cycle and indirect soil fertilizer, it may also disrupt the natural balance of life in regions exposed to high frequency waves up to 100 GHz or beyond for a long time.…”

Section: Resultsmentioning

confidence: 99%

5 GHZ Wi Fi Effects on Escherichia Coli, Caenorhabditis Elegans and Human Neuroblastoma Cells

Dinç

Ilyas

Kaygusuz

et al. 2021

Mugla Journal of Science and Technology

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The use of 5 GHz Wi-Fi has spread and potential effects on microorganisms and human health are still under investigation. To investigate the possible effects for that, experiments were performed using three different microorganisms, Escherichia coli, SH-SY5Y human neuroblastoma cells and Caernohabditis elegans. The maximum allowed temperature for the Wi-Fi Access Point (AP) was considered in the measurement setup in order not to harm the internal circuitry of the router which can result impact the performance of the router. Suspension turbidity, optical density measurements and simultaneous counting of E. coli were performed. It was observed that 5 GHz Wi-Fi exposure slow down the E. coli growth rate at the same temperature conditions with sham exposed groups especially after the first 6 h. 5 GHz Wi-Fi induced decrease in cell viability remarkably for the cells seeded at densities 20,40, 100 (×10 3) and viability values were varied between 20 and 30%. C. elegans which is a nematode, plays key role in nutrient cycling and soil fertilization was affected by the radiation and egg-laying rates changed by 27.49%.

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“…The first step in studying the effect of 2.45 GHz on food products is to study the effect that microwaves have on simple bacterial suspensions. In effect, many scientific investigations have been done either on bacterial populations [9][10][11] or on medical structures, 12,13 but most of the present information is about systems without temperature control; this, however, is an important aspect because, even though the temperature increase may not be very high, it could be sufficient enough to induce thermal effects on bacteria and also on proteins (for example, Bohr and Bohr 14 reported that MW radiation enhances thermal folding and unfolding of the globular protein b-lactoglobulin in the 5-44 C range).…”

Section: Introductionmentioning

confidence: 99%

The effect of low‐power microwaves on the growth of bacterial populations in a plug flow reactor

Carta

Desogus

2009

AIChE Journal

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A plug flow reactor (PFR) which permits the growth of bacteria in a microwave environment is described. The apparatus is used to analyse the effect of microwave radiation at the frequency of 2.45 GHz and at 37°C on the growth rate of three bacterial species: Bacillus clausii, Pseudomonas aeruginosa, and Staphylococcus aureus. The growth constant is determined for reacting mixtures irradiated with microwaves of different powers ranging from 0 to 400 mW. Analyses show that (a) the apparatus is able to perform the experimental runs maintaining an aseptic environment; (b) under the microwave power levels examined in the present study, no effects are detected for the Bacillus clausii and Pseudomonas aeruginosa species, while for the Staphylococcus aureus species, a few small effects are found (a minimum value of the growth constant at 200 mW and an increase of the constant between 200 and 400 mW). © 2009 American Institute of Chemical Engineers AIChE J, 2010

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Effects of radio-frequency fields on bacterial cell membranes and nematode temperature-sensitive mutants

Cited by 12 publications

References 24 publications

Continuous wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16‐1 heat‐shock gene expression in Caenorhabditis elegans

Continuous wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16‐1 heat‐shock gene expression in Caenorhabditis elegans

5 GHZ Wi Fi Effects on Escherichia Coli, Caenorhabditis Elegans and Human Neuroblastoma Cells

The effect of low‐power microwaves on the growth of bacterial populations in a plug flow reactor

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