Effects of Pu-erh ripened tea on hyperuricemic mice studied by serum metabolomics

Zhao, Ran; Chen, Dong; Wu, Hualing

doi:10.1016/j.jchromb.2017.10.002

Cited by 19 publications

(17 citation statements)

References 44 publications

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“…Tian et al [79] studied the effect of Xiaoyaosan, a herbal medicine, in depressed patients. The GC-MS derivatization settings are similar to those of the untargeted approaches described in [82] and [9]. Nowadays, the derivatization step will be less time consuming due to the recent improvements [76], as for example the SPAD technology.…”

Section: Gc-msmentioning

confidence: 99%

Analytical Techniques for Metabolomic Studies: A Review

et al. 2019

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Metabolomics is the comprehensive study of small-molecule metabolites. Obtaining a wide coverage of the metabolome is challenging because of the broad range of physicochemical properties of the small molecules. To study the compounds of interest spectroscopic (NMR), spectrometric (MS) and separation techniques (LC, GC, supercritical fluid chromatography, CE) are used. The choice for a given technique is influenced by the sample matrix, the concentration and properties of the metabolites, and the amount of sample. This review discusses the most commonly used analytical techniques for metabolomic studies, including their advantages, drawbacks and some applications.

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Section: Gc-msmentioning

confidence: 99%

Analytical Techniques for Metabolomic Studies: A Review

et al. 2019

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“…At present, the incidence of hyperuricemia is increasing worldwide with increased occurrence in younger individuals, leading to a huge economic and social burden [ 6 ]. Since humans lack the corresponding enzymes, uricase, and allantoin, in vivo metabolism of purines involves xanthine oxidase-mediated decomposition to xanthines and finally conversion to uric acid [ 7 , 8 ]. Therefore, lowering the uric acid level is a key to treating patients with hyperuricemia.…”

Section: Introductionmentioning

confidence: 99%

“…Metabolomics is the study of changes in metabolites produced by biological systems (the cell, tissue, or organism) after they have been stimulated. Small molecules are its target research objective, including their production and metabolism as end result of a series of events; therefore, metabolomics defines the state of biological systems more accurately [ 8 , 12 , 15 , 16 ]. Currently, metabolomics is widely used in the field of TCM [ 7 , 11 , 17 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

Study of the Treatment Effects of Compound Tufuling Granules in Hyperuricemic Rats Using Serum Metabolomics

Zhang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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The study aimed to investigate the mechanism of the effect of Compound Tufuling Granules (CTG) to lower the serum uric acid level in a rat model of hyperuricemia. The rat model was established by administering hypoxanthine through oral gavage and potassium oxonate through intraperitoneal injection. Rats were divided into the normal group, model group, CTG group, and allopurinol group. Serum uric acid, creatinine, urea nitrogen, and inflammatory cytokine levels were determined in each group. In the model group, ultrahigh performance liquid chromatography-mass spectrometry was used to analyze the metabolic profiles and delineate the action mechanism of CTG; in addition, the orthogonal projection method was used to perform latent structure-discrimination analysis to screen the related metabolites. The results indicated significant differences in the metabolic profiles between the model and normal groups. A total of seven related metabolites were identified through screening in the model group, mainly related to the pathways of bile secretion, pyrimidine, purine, and phenylalanine metabolism, pantothenate and CoA biosynthesis, and pentose and glucuronate interconversions; these related pathways were reversed in the CTG group. In the metabolic networks, uracil and acetyl-coenzyme A were the nodal molecules. In addition, the test results of the evaluation of serum biochemical and inflammatory factors confirmed that CTG had significant effect in reducing the levels of serum uric acid and protecting renal function. These results confirmed that CTG primarily regulated the recruitment of nodal molecules to achieve anti-inflammatory effects, reduced uric acid level, and renal protection.

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“…Allopurinol is recognised as the first‐line drug for the treatment of HUA, but it has caused serious side effects during clinical use, including drug allergies, hepatotoxicity and nephrotoxicity. As it has been previously reported that herbal medicine, such as Pu‐erh ripened tea, Konjac glucomannan, Corylopsis coreana Uyeki and Smilax china L., are more effective and safer for treating HUA and gout. In herbal extract, caffeic acid and rosmarinic acid were verified to be potent antihyperuricemic agents .…”

Section: Discussionmentioning

confidence: 88%

Antihyperuricemic and nephroprotective effects of extracts from Orthosiphon stamineus in hyperuricemic mice

Wang

Sun³

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives To investigate the antihyperuricemia and nephroprotective effects of Orthosiphon stamineus extracts on hyperuricemia (HUA) mice and explore the potential mechanisms. Methods Orthosiphon stamineus extracts were extracted using 50% ethanol and enriched using ethyl acetate, and characterised utilising UPLC/ESI‐MS. A potassium oxonate (PO) induced hyperuricemic mouse model was used to evaluate antihyperuricemia and nephroprotective effects of O. stamineus ethyl acetate extracts (OSE). Key findings Eight constituents from OSE were identified and OSE treatment ameliorated HUA by regulating key indicators of kidney dysfunction and xanthine oxidase, adenosine deaminase activity and urate transporters in hyperuricemic mice. Moreover, in renal histopathology analysis, OSE significantly alleviated kidney injury. Conclusions These findings demonstrate that OSE has antihyperuricemic and nephroprotective effects on PO‐induced HUA mice and those results indicate that OSE could be a safe and effective agent or functional ingredient for treating HUA.

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Effects of Pu-erh ripened tea on hyperuricemic mice studied by serum metabolomics

Cited by 19 publications

References 44 publications

Analytical Techniques for Metabolomic Studies: A Review

Analytical Techniques for Metabolomic Studies: A Review

Study of the Treatment Effects of Compound Tufuling Granules in Hyperuricemic Rats Using Serum Metabolomics

Antihyperuricemic and nephroprotective effects of extracts from Orthosiphon stamineus in hyperuricemic mice

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