Effects of protease enzyme supplementation and varying levels of amino acid inclusion on productive performance, egg quality, and amino acid digestibility in laying hens from 30 to 50 weeks of age

Poudel, Ishab; Hodge, V.R.; Wamsley, K.G.S.; Roberson, K.D.; Adhikari, P.A.

doi:10.1016/j.psj.2022.102465

Poultry Science

2023

DOI: 10.1016/j.psj.2022.102465

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Effects of protease enzyme supplementation and varying levels of amino acid inclusion on productive performance, egg quality, and amino acid digestibility in laying hens from 30 to 50 weeks of age

Ishab Poudel¹,

V.R. Hodge²,

K.G.S. Wamsley³

et al.

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Cited by 3 publications

(1 citation statement)

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“…The relative weight of the digestive tract and immune organs several variables impact this, including ration protein concentration, ability to digest, feed, and chicken maturity. [5].…”

mentioning

confidence: 99%

Effect of Addition Sinbiotic Isolate Pediococcus pentosaceus Strain N6 and Cassava Peel on Immunity Organs and Total Microbes in the Small Intestine of Super Native Chicken

Umar,

Hulu

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Factory feed is often added with antibiotics as a growth promoter. Giving synthetic antibiotics is very dangerous for health. This study aims to determine effect of addition synbiotic isolate Pediococcus pentosaceus strain N6 and cassava peel flour on the immune organs and total microbes in the small intestine of super native chickens. This research was conducted in Pancur Batu from September - December 2022. The design used was a completely randomized design (CRD) with 4 treatments 5 replicates so that 20 experimental units were obtained. The super native chicken used were 100 DOC. The treatments given were P0 (basal ration without sinbiotic), P1 (basal ration + sinbiotic 150 ml/kg ration), P2 (basal ration + sinbiotic 300 ml/kg ration), and P3 (basal ration + sinbiotic 450 ml/kg ration). Parameters studied were weight percentage of immune organs and total microbial colonies. The results of this study indicated that the addition of synbiotic isolate Pediococcus pentosaceus strain N6 and fermented cassava peel flour up to a dose of 450 ml/kg as an alternative feed for native chickens increased percentage weight of spleen, thymus, bursa fabrisius, and total microbial colony of super native chickens.

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“…The relative weight of the digestive tract and immune organs several variables impact this, including ration protein concentration, ability to digest, feed, and chicken maturity. [5].…”

mentioning

confidence: 99%

Effect of Addition Sinbiotic Isolate Pediococcus pentosaceus Strain N6 and Cassava Peel on Immunity Organs and Total Microbes in the Small Intestine of Super Native Chicken

Umar,

Hulu

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Effects of different supplemental levels of protease DE200 on the production performance, egg quality, and cecum microflora of laying hens

Cai,

Liu,

et al. 2024

Journal of Animal Science

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This study aimed to investigate the effects of different levels of the protease DE200TM on the performance, egg quality, organ index and cecum microflora of Hy-line W36 laying hens. In this experiment, a total of 180 laying hens aged 300 days were randomly divided into three treatment groups and fed diets containing 0, 100 or 200 g/t DE200. The experimental period was eight weeks, including two weeks of prefeeding and six weeks of the formal experiment. Regular feeding was performed thrice a day and eggs were collected twice daily, and the feed intake and the egg quality were recorded. The results showed that in terms of production performance, dietary supplementation with different levels of DE200 significantly increased egg production (EP) (P < 0.05) and significantly decreased the feed conversion ratio (FCR) (P < 0.05) and average daily feed intake (ADFI) (P < 0.05) without affecting egg weight (EW). In addition, the addition of DE200 significantly reduced the egg breakage rate (P < 0.05) and tended to increase the Haugh unit and decrease the water content of the yolk (P > 0.05). In the cecal microflora, the addition of DE200 increased the proportions of Bacteroidetes and Firmicutes at the phylum level while reducing the proportion of Fusobacteria. Furthermore, at the genus level, the addition of DE200 increased the proportions of Bacteroides and Faecalibacterium and reduced the proportion of Megamonas. This study suggested that the protease DE200 can be used as a feed supplement to improve the production performance of laying hens.

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Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry

Khlebodarova,

Bogacheva,

Zadorozhny

et al. 2024

Microorganisms

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In the 1980s, Escherichia coli was the preferred host for heterologous protein expression owing to its capacity for rapid growth in complex media; well-studied genetics; rapid and direct transformation with foreign DNA; and easily scalable fermentation. Despite the relative ease of use of E. coli for achieving the high expression of many recombinant proteins, for some proteins, e.g., membrane proteins or proteins of eukaryotic origin, this approach can be rather ineffective. Another microorganism long-used and popular as an expression system is baker’s yeast, Saccharomyces cerevisiae. In spite of a number of obvious advantages of these yeasts as host cells, there are some limitations on their use as expression systems, for example, inefficient secretion, misfolding, hyperglycosylation, and aberrant proteolytic processing of proteins. Over the past decade, nontraditional yeast species have been adapted to the role of alternative hosts for the production of recombinant proteins, e.g., Komagataella phaffii, Yarrowia lipolytica, and Schizosaccharomyces pombe. These yeast species’ several physiological characteristics (that are different from those of S. cerevisiae), such as faster growth on cheap carbon sources and higher secretion capacity, make them practical alternative hosts for biotechnological purposes. Currently, the K. phaffii-based expression system is one of the most popular for the production of heterologous proteins. Along with the low secretion of endogenous proteins, K. phaffii efficiently produces and secretes heterologous proteins in high yields, thereby reducing the cost of purifying the latter. This review will discuss practical approaches and technological solutions for the efficient expression of recombinant proteins in K. phaffii, mainly based on the example of enzymes used for the feed industry.

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Effects of protease enzyme supplementation and varying levels of amino acid inclusion on productive performance, egg quality, and amino acid digestibility in laying hens from 30 to 50 weeks of age

Cited by 3 publications

References 50 publications

Effect of Addition Sinbiotic Isolate Pediococcus pentosaceus Strain N6 and Cassava Peel on Immunity Organs and Total Microbes in the Small Intestine of Super Native Chicken

Effect of Addition Sinbiotic Isolate Pediococcus pentosaceus Strain N6 and Cassava Peel on Immunity Organs and Total Microbes in the Small Intestine of Super Native Chicken

Effects of different supplemental levels of protease DE200 on the production performance, egg quality, and cecum microflora of laying hens

Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry

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