Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Tamara M. Khlebodarova,
Natalia V. Bogacheva,
Andrey V. Zadorozhny
et al.
Abstract:In the 1980s, Escherichia coli was the preferred host for heterologous protein expression owing to its capacity for rapid growth in complex media; well-studied genetics; rapid and direct transformation with foreign DNA; and easily scalable fermentation. Despite the relative ease of use of E. coli for achieving the high expression of many recombinant proteins, for some proteins, e.g., membrane proteins or proteins of eukaryotic origin, this approach can be rather ineffective. Another microorganism long-used and… Show more
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