Effects of propofol and carbon dioxide on acid-base balance in Siberian sturgeon

Abstract

The aim of this study was to compare physiological responses in Siberian sturgeon (Acipenser baeri) induced by propofol and CO 2 anaesthesia. Two procedures were applied during the experiment. In procedure I, blood samples were collected immediately after exposure (1, 2, 5, 10 min) to the anaesthetic. In procedure II, fish were exposed to the anaesthetic for 10 minutes and then were moved to anaesthetic free water. Blood was sampled after 5, 10, 20 or 30 min of recovery time. Gasometrical and biochemical analyses were performed on collected blood.In CO 2 anaesthetized fish strong hypercapnic acidosis was revealed. The drop of the HCO 3 -/CO 2 ratio, from 28:1 in control fish up to 4:1 in CO 2 anaesthetized ones, proved that the compensation mechanism is not capable of preventing acidosis during CO 2 anaesthesia in Siberian sturgeon. In contrast, only moderate, respiratory acidosis occurred in sturgeons anaesthetised with propofol. Hypercapnic acidosis during CO 2 anaesthesia was followed by a fourfold increase of ammonia level in the blood. Glucose level, increasing only during recovery time, indicates that a secondary stress response occurred when awareness of anaesthetized fish had been restored.

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