Effects of probiotics and encapsulated probiotics on enteric methane emission and nutrient digestibility in vitro

Abstract

The objective of this study was to evaluate the effects of probiotics and encapsulated probiotics on enteric methane production and the in vitro nutrient digestibility in ruminants. The probiotics used were from the group of lactic acid bacteria (LAB). The experiments were conducted in three dietary treatments (control diet, probiotics addition, and encapsulated probiotics addition) and three replicates. Each replicate was performed at a different week. The experimental diets were incubated in the in vitro rumen fermentation system for 72 h. Parameters observed were pH, total gas production, methane production, total volatile fatty acids, NH3, and the in vitro dry and organic matter digestibility (IVDMD and IVOMD, respectively). Data were analyzed using variance analysis and continued with the Duncan multiple range test to compare among the different treatment means. Results showed both the probiotics and the encapsulated probiotics decreased (P<0.05) the methane production by 6.1 and 33.1% compared to the control diet, respectively. Furthermore, these probiotics and encapsulated probiotics increased (P<0.05) total gas production by 15.7 and 233% than that of control, respectively. The TFVAs, IVDMD, IVOMD, and NH3 values of the diet supplemented with probiotics were higher than the encapsulated probiotics (P<0.05). It can be concluded that both the probiotics and the encapsulated probiotics effectively mitigate the in vitro methane production while simultaneously enhancing the total gas production.

Probiotics: Symbiotic Relationship with the Animal Host

“…Moreover, García et al [46] reported that the Lactobacillus pentosus strain LB-31 demonstrated antagonistic activity and antimicrobial susceptibility, as well as tolerance to bile salts, pH changes and hydrophobicity. Furthermore, Abdelbagi et al [47] reported that probiotic strains (encapsulated or not) in in vitro studies decreased methane production and increased the total gas production in relation to the control treatment.…”

“…This review article has 195 references: 192 articles published in peer-reviewed scientific journals, two chapters of scientific books from prestigious publishers and a reference from an international organization. For the effect of probiotics in in vitro studies, 20 references were used [30,[36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54], which demonstrate the desirable probiotic characteristics of bacterial isolates for survival in the gastrointestinal tract, the production of metabolites such as bacteriocin and its antagonistic effect on pathogens. It is necessary to replicate the in vitro and in vivo results, as well as to find the specific mode of action (biochemical reactions) of each proposed probiotic candidate in the animal host.…”

Methanotrophy: A Biological Method to Mitigate Global Methane Emission

“…Nevertheless, an approach that is progressive, such as probiotics, involving methane measurements and mitigation strategies with an emphasis more on the biological reduction of methane emissions that occur due to direct-fed microbes, could be considered as a methane mitigation approach towards sustainability [85][86][87]. Researchers have developed numerous nutritional technologies to minimize CH 4 production, including direct inhibition techniques [88], feeding ruminants with additives [89], propionate enhancers [90], methane oxidizers [91], probiotics [92], defaunation [93], and manipulation of ruminant diets and hormones [84,94]. The study conducted by Abdelbagi et al [92] aimed to evaluate the impact of probiotics and encapsulated probiotics on enteric methane production and in vitro nutrient digestibility in ruminants.…”

“…Researchers have developed numerous nutritional technologies to minimize CH 4 production, including direct inhibition techniques [88], feeding ruminants with additives [89], propionate enhancers [90], methane oxidizers [91], probiotics [92], defaunation [93], and manipulation of ruminant diets and hormones [84,94]. The study conducted by Abdelbagi et al [92] aimed to evaluate the impact of probiotics and encapsulated probiotics on enteric methane production and in vitro nutrient digestibility in ruminants. The probiotics utilized were from the group of lactic acid bacteria.…”

RUMEN METHANE PRODUCTION AND ITS REDUCTION USING NUTRITIONAL FACTORS (review)

“…Так, результаты in vitro у жвачных животных показали, что обычные и инкапсулированные пробиотики из группы молочнокислых бактерий снижали выработку метана соответственно на 6,1 и 33,1 % по сравнению с контролем. При этом авторы отмечали увеличение общего газообразования на 15,7 и 23,3 % при использовании тех же пробиотиков (71). В работе G. Guo и соавт.…”