Effects of probiotic bacteria and their genomic DNA on TH1/TH2-cytokine production by peripheral blood mononuclear cells (PBMCs) of healthy and allergic subjects

Ghadimi, Darab; Fölster‐Holst, Regina; Vrese, Michael de; Winkler, Petra; Heller, Knut J.; Schrezenmeir, J.

doi:10.1016/j.imbio.2008.02.001

Cited by 159 publications

(126 citation statements)

References 49 publications

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“…The resultant TH2 shifted response of allergic patients indicates the role of impaired TH1/TH2 balance in allergic diseases and was proved by (Pochard et al, 2002;Ghadimi et al, 2008), their experiments included challenging of PBMCs of patients allergic to house dust mite (Dpt allergen). IFN-γ mRNA expression and production was reported to be reduced in atopic subjects (Parronchi et al, 1991) during in vitro stimulation experiments.…”

Section: Discussionmentioning

confidence: 98%

“…Lactis G50 killed by heat to induce cytokine production of macrophages in culture, was previously proved by Kimoto et al (2004), it was observed that this strain continued to induce cytokine production, suggesting that such activity is associated with elements on the bacterial wall. Ghadimi et al (2008) proved that Lactobacillus rhamnosus GG, Lactobacillus gasseri (PA16/8), Bifidobacterium bifidum (MP20/5) and Bifidobacterium longum (SP07/3), as well as their genomic DNA, dosedependently modulated the TH1/TH2 response to allergens. DNA seemed to contribute to about 50% of the immunomodulatory effects exerted by live bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro studies using PBMCs from allergic patients have shown reduced expression of TH2-associated cytokines (IL-4 and IL-5) on stimulation with total extract of Dermatophagoides pteronyssinus (house dust mite) and prior treatment with lactic acid bacteria strains such as Lactobacillus plantarum (Pochard et al, 2002). Both Lactococcus lactis and Lactobacillus plantarum, induce high levels of IL-12 and IFNγ, suppressing Th2 differentiation (Ghadimi et al, 2008). In contrast, suppression of contact dermatitis in mice has been shown to be mediated by a Lactobacillus acidophilus L92-dependent generation of Tregs (Shah et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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The Immunomodulatory Effects of Probiotic Bacteria on Peripheral Blood Mononuclear Cells (Pbmcs) of Allergic Patients

Sheikh¹,

Shalaby²,

Hafez³

et al. 2014

American Journal of Immunology

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Allergic diseases represent major health burden. An allergic reaction is characterized by a disrupted Thelper 1⁄T-helper 2 balance toward a preferential allergen specifically induced TH2 cytokine profile, causing allergic inflammation Probiotic bacteria have various benificial effects in many pathologic situation. Studies have shown that the bacteria present in the intestinal micro flora play a role in the TH1/TH2 balance and its modulation can promote the control of infectious and immune processes. Testing the effects of probiotic bacteria on TH1/TH2 cytokine production by peripheral blood mononuclear cells of allergic patients and control subjects. This study included 24 patients allergic to date pollen and 16 healthy control subjects. PBMC of both groups were separated and cultured for 72 h with date pollen allergen (home-made) in the presence or absence of Lactobacillus rhamnosus ATCC 7469 (Living and dead) and Cphycocyanin (extracted from Spirulina platensis). The cell culture supernatants were collected to measure Interlukin 4 and Interferon gamma by quantitative ELISA. Incubation of PBMCs of allergic patients with living Lactobacillus rhamnosus ATCC 7469 showed marked reduction in IL4 production (median IL4 concentarion = 3.9 pg.) compared to PBMCs callenged with pollen alone (mediam IL4 conentration = 52.6 pg). When PBMC were incubated with living Lactobacillus rhamnosus in absence of allergen significant increase in and IFNγ (median concentration = 42.75 pg.) was obtained, compared to PBMC challenged with allergen alone (median = 22.8 pg). When PBMCs incubated with heat killed Lactobacillus rhamnosus either in presence or absence of the offending allergen, marked reduction in IL4 production was obtained (median = 10.6, 3.6 pg respectively) compared to PBMC incubated with allergen alone (median = 52.6 pg). When PBMCs incubated with dead Lactobacillus rhamnosus, marked increase in IFNγ production was obtained (median = 49 pg) when compared to IFNγ production by PBMC challenged with allergen (median 22.8 pg). PBMCs challenged with PC in the presence or absence of allergen showed marked decrease of IL4 production (median = 19.8, 17 pg respectively) when compared to PBMC incubated with the offending allergen alone (median = 52.6 pg). PBMCs incubated with PC showed significant increase of and IFNγ production (median= 319.6 pg) when compared to PBMC incubated with the offending allergen alone (median = 22.8 pg). Conclusion Lactobacillus rhamnosus ATCC 7469 and C-phycocyanin (extracted from Spirulina platensis) inversed the TH1: TH2 polarization in allergic patients and could be a promissing line of treatmen.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Immunomodulatory Effects of Probiotic Bacteria on Peripheral Blood Mononuclear Cells (Pbmcs) of Allergic Patients

Sheikh¹,

Shalaby²,

Hafez³

et al. 2014

American Journal of Immunology

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“…One of the preconditions for a bacterial strain to be called probiotic is the strain's ability to survive in the gastrointestinal environment, although the importance of viability for the beneficial effects of probiotics has not been well defined since inactivated and dead cells can also have immunological and health-promoting effects (Ghadimi et al, 2008;Lopez et al, 2008). Moreover, there are significant technological challenges associated with the introduction and maintenance of high numbers of probiotic microorganisms in foods that depend on the form of the probiotic inoculant, and with the viability and maintenance of probiotic characteristics in the food product up to the time of consumption.…”

Section: Probiotic Cheesesmentioning

confidence: 99%

Probiotics in Dairy Fermented Products

Araújo¹,

Pires

Pinto

et al. 2012

Probiotics

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“…One animal study (Ohno et al, 2005) and three in vitro studies (Ghadimi et al, 2008;2010a;2010b) explored the effects of the microorganisms on the immune response to allergens/antigens. Three of these studies examined the effects of a single strain and only the in vitro …”

Section: Scientific Substantiation Of the Claimed Effect (Id 931)mentioning

confidence: 99%

Scientific Opinion on the substantiation of health claims related to a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 and maintenance of upper respiratory tract defence against pathogens (ID 931, further assessment) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

Products¹

2012

EFS2

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The claimed effect, which is proposed for further assessment, is "supports the defence against pathogens in the upper respiratory tract resulting in a decrease in duration and severity of common cold symptoms". The proposed target population is the general adult population. The Panel considers that maintenance of upper respiratory defence against pathogens is a beneficial physiological effect.In the only human intervention study provided, the effect of consumption of the bacterial preparation on the incidence, severity and duration of common colds was assessed in healthy adults. The Panel notes that no information is provided about the validity of the questionnaire used to assess the incidence, severity or duration of respiratory tract infections, that the symptoms used for the diagnosis of common cold episodes are non-specific, and that the presence of one or more of these symptoms is not an appropriate measure of respiratory tract infections in the study population. The Panel also notes that no information has been provided in the publication about the use of rescue medication, which may have confounded the results. The Panel considers that no conclusions can be drawn from this study for the scientific substantiation of the claim.The Panel notes that no human intervention studies have been provided from which conclusions could be drawn for the scientific substantiation of the claim, and that the animal and in vitro studies which were provided cannot be used for the scientific substantiation of the claim as their results cannot predict the occurrence of an effect of a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 in vivo in humans.On the basis of the data presented, the Panel concludes that a cause and effect relationship has not been established between the consumption of a combination of Lactobacillus gasseri PA 16/8, Bifidobacterium bifidum M 20/5 and Bifidobacterium longum SP 07/3 and maintenance of upper respiratory tract defence against pathogens.

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Effects of probiotic bacteria and their genomic DNA on TH1/TH2-cytokine production by peripheral blood mononuclear cells (PBMCs) of healthy and allergic subjects

Cited by 159 publications

References 49 publications

The Immunomodulatory Effects of Probiotic Bacteria on Peripheral Blood Mononuclear Cells (Pbmcs) of Allergic Patients

The Immunomodulatory Effects of Probiotic Bacteria on Peripheral Blood Mononuclear Cells (Pbmcs) of Allergic Patients

Probiotics in Dairy Fermented Products

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