Effects of Probiotic Bacillus as an Alternative of Antibiotics on Digestive Enzymes Activity and Intestinal Integrity of Piglets

Abstract

The previous study in our team found that supplementation of probiotic Bacillus amyloliquefaciens (Ba) instead of antibiotics promote growth performance of piglets. Hence, the present study was carried out to further demonstrate the effect of Ba replacement of antibiotics on digestive and absorption enzyme activity and intestinal microbiota population of piglets. A total of 90 piglets were selected and divided into three groups: G1 group was fed with basal diet supplemented with 150 mg/Kg aureomycin, G2 group was fed with 1 × 108 cfu/Kg Ba and half dose of aureomycin, G3 group was used the diet with 2 × 108cfu/Kg Ba replaced aureomycin. Each treatment had three replications of 10 pigs per pen. Results indicated that Ba replacement significantly increased the activities of amylase, disaccharides and Na+/K+-ATPase. And chymotrypsin activity in different section of intestine was dramatically enhanced in half replacement of aureomycin with Ba. Moreover, Ba replacement maintained the intestinal integrity with the significantly decreased activity of DAO compared with aureomycin group. Besides, supplementation with Ba increased the β-diversity of intestinal microbiota. Taken together, the current study indicated that diet supplementation with Ba instead of aureomycin increased the growth performance of piglets by improving the digestive and absorb enzyme activities, enhancing the intestinal integrity and regulating the population of intestinal micrbiota.

Evaluation of Bacillus licheniformis-Fermented Feed Additive as an Antibiotic Substitute: Effect on the Growth Performance, Diarrhea Incidence, and Cecal Microbiota in Weaning Piglets

“…Body weight and gut microbiota have not been found to be affected by the supplementation of Bacillus species-based probiotics in combination with antibiotic administration in piglets during lactation and post-weaning [33]. Replacing all the antibiotics or the half replacement of antibiotics with B. amyloliquefaciens has been found to increase growth performance, antioxidant capacity, and digestive enzyme activity compared with the groups treated with antibiotics alone [18,19]. However, the half and total replacement of antibiotics with B. amyloliquefaciens was found to not alter the α-diversity of the microbial population in the feces of weaning piglets [19].…”

“…Therefore, it is imperative to explore effective alternatives to antibiotic growth promoters in infectious disease prevention in animal production. The effect of replacing or reducing antibiotics with probiotics on the performance and gut microbiota of pigs has been increasingly investigated [18,19,23,33]. It has been reported that the simultaneous supplementation of B. licheniformis and antibiotics cannot improve the growth performance of weaning piglets [23].…”

“…Replacing all the antibiotics or the half replacement of antibiotics with B. amyloliquefaciens has been found to increase growth performance, antioxidant capacity, and digestive enzyme activity compared with the groups treated with antibiotics alone [18,19]. However, the half and total replacement of antibiotics with B. amyloliquefaciens was found to not alter the α-diversity of the microbial population in the feces of weaning piglets [19]. However, a recent study demonstrated that bacterial species richness and evenness in the feces decreased in weaning piglets fed BLF or antibiotics [11].…”

“…The combination of antibiotics and probiotics, as well as comparisons of these feed additives, have been evaluated in broilers and piglets [18][19][20]. The half replacement of antibiotics with Bacillus amyloliquefaciens has been found to improve the growth performance, antioxidant activity, and digestive enzyme activity in weaning piglets [18,19]. However, to the best of our knowledge, limited information is available in terms of the parallel supplementation of bacitracin and BLF in the diet of weaning piglets.…”

Effects of probiotic BaSC06 on intestinal digestion and absorption function, antioxidant capacity and macrophage polarization and microbiota composition in fattening pigs

“…Bacillus amyloliquefaciens is preferred to be a feed supplement due to its higher resistance to harsh environments [8]. Besides, our previous studies based on piglets have demonstrated that probiotic BaSC06 signi cantly increase growth performance and antioxidant status by improving the digestive and absorb enzyme activities, antioxidant capacity, and intestinal autophagy; enhancing the intestinal integrity by improving intestinal mucosa structure, tight junctions and regulating the population of intestinal microbiota; increasing immune function by activating the TLRs signaling pathway [9,10,11].…”