Effects of pro-inflammatory cytokines, lipopolysaccharide and COX-2 mediators on human colonic neuromuscular function and epithelial permeability

Safdari, B.K.; Sia, Tiong Cheng; Wattchow, David; Smid, Scott D.

doi:10.1016/j.cyto.2016.04.017

Cited by 16 publications

(21 citation statements)

References 33 publications

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“…In experimental colitis models, as well as in human IBD, the intestinal inflammatory process is directly associated with the induction of oxidative stress, which reduces the cellular antioxidant capacity. The excessive production of free radicals can react with the fatty acids of the cell membrane, in turn leading to lipid peroxidation, and thus contributing to intestinal oxidative damage (Safdari et al, 2016;Nikkhah-Bodaghi et al, 2019). As a result, another mechanism which could justify the beneficial effects of HEBP on these experimental colitis models can be associated with the antioxidant properties which it may exert, mostly due to the presence of flavonoids, especially quercetin and kaempferol.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Chemopreventive Effects of Bryophyllum pinnatum (Lamarck) Leaf Extract in Experimental Colitis Models in Rodents

et al. 2020

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pro-inflammatory and oxidative mediators, chemokines, and cell adhesion molecules. This immunomodulatory property was proposed that one of the possible action mechanisms of extract. An improvement in intestinal damage was also associated with a reduction in oxidative stress and infiltration of leukocytes, as evidenced by the reduction in malonaldialdehyde and myeloperoxidase activity and increase in total glutathione in the colonic tissue. Moreover, the extract improved the cytoarchitecture of the colonic tissue and the integrity of the intestinal epithelial barrier by restoring the expression of the proteins associated with mucosa protection. In view of the beneficial effects showed by the B. pinnatum leaf extract in preclinical rodent models of colitis there is the potential to conduct some future clinical studies to ensure safe and effective development of a phytotherapeutic treatment for human inflammatory bowel diseases.

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Chemopreventive Effects of Bryophyllum pinnatum (Lamarck) Leaf Extract in Experimental Colitis Models in Rodents

et al. 2020

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“…Combined these results indicate IL-1β may have a role in colonic disease states including dysmotility, diarrhoea and lower abdominal pain. However, we previously observed that incubation of human colonic resections with IL-1β did not affect cholinergic contractility of longitudinal muscle strips [ 36 ]. Here we superfused healthy colonic tissue with IL-1β at a concentration consistent with what we have previously used to excite colonic sensory afferent nerves and similar to the whole colon concentration we measured in TNBS colitic mice.…”

Section: Discussionmentioning

confidence: 99%

Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis

et al. 2018

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BackgroundInflammatory Bowel Disease (IBD) is characterized by overt inflammation of the intestine and is typically accompanied by symptoms of bloody diarrhea, abdominal pain and cramping. The Colonic Migrating Motor Complex (CMMC) directs the movement of colonic luminal contents over long distances. The tri-nitrobenzene sulphonic acid (TNBS) model of colitis causes inflammatory damage to enteric nerves, however it remains to be determined whether these changes translate to functional outcomes in CMMC activity. We aimed to visualize innate immune cell infiltration into the colon using two-photon laser scanning intra-vital microscopy, and to determine whether CMMC activity is altered in the tri-nitro benzene sulphonic (TNBS) model of colitis.MethodsEpithelial barrier permeability was compared between TNBS treated and healthy control mice in-vitro and in-vivo. Innate immune activation was determined by ELISA, flow cytometry and by 2-photon intravital microscopy. The effects of TNBS treatment and IL-1β on CMMC function were determined using a specialized organ bath.ResultsTNBS colitis increased epithelial barrier permeability in-vitro and in-vivo. Colonic IL-1β concentrations, colonic and systemic CD11b+ cell infiltration, and the number of migrating CD11b+ cells on colonic blood vessels were all increased in TNBS treated mice relative to controls. CMMC frequency and amplitude were inhibited in the distal and mid colon of TNBS treated mice. CMMC activity was not altered by superfusion with IL-1β.ConclusionsTNBS colitis damages the epithelial barrier and increases innate immune cell activation in the colon and systemically. Innate cell migration into the colon is readily identifiable by two-photon intra-vital microscopy. CMMC are inhibited by inflammation, but this is not due to direct effects of IL-1β.

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“…IBD is associated with an increase in pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, IL-17, tumour necrosis factor (TNF)-α, prostaglandins and nitric oxide (NO), which may impair colonic muscle contraction and barrier function. These inflammatory mediators are important for the generation and maintenance of inflammation [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Intestinal anti-inflammatory effects of goat whey on DNBS-induced colitis in mice

et al. 2017

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This study evaluated the intestinal anti-inflammatory effects of goat whey in a mouse model of colitis induced by 2,4-dinitrobenzenesulfonic acid that resembles human IBD. At a concentration of 4 g/kg/day, the goat whey improved the symptoms of intestinal inflammation, namely by decreasing the disease activity index, colonic weight/length, and leukocyte infiltration. Moreover, goat whey inhibited NF-κB p65 and p38 MAPK signaling pathways and consequently down-regulated the gene expression of various proinflammatory markers such as IL-1β, IL-6, IL-17, TNF-α, iNOS, MMP-9, ICAM-1. Also, goat whey increased the expression of proteins such as mucins, occludin proteins and cytokine signalling suppressors. The immunomodulatory properties of goat whey were also evaluated in vitro using the murine macrophage cell line Raw 264 and CMT-93 cells derived from mouse rectum carcinomas. The results revealed the ability of goat whey to inhibit the production of NO and reduce IL-6 production in LPS-stimulated cells. In conclusion, goat whey exhibited anti-inflammatory effects in the DNBS model of intestinal inflammation, and these observations were confirmed by its immunomodulatory properties in vitro. Together, our results indicate that goat whey could have applications for the treatment of IBD.

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Effects of pro-inflammatory cytokines, lipopolysaccharide and COX-2 mediators on human colonic neuromuscular function and epithelial permeability

Cited by 16 publications

References 33 publications

Anti-Inflammatory and Chemopreventive Effects of Bryophyllum pinnatum (Lamarck) Leaf Extract in Experimental Colitis Models in Rodents

Anti-Inflammatory and Chemopreventive Effects of Bryophyllum pinnatum (Lamarck) Leaf Extract in Experimental Colitis Models in Rodents

Colonic migrating motor complexes are inhibited in acute tri-nitro benzene sulphonic acid colitis

Intestinal anti-inflammatory effects of goat whey on DNBS-induced colitis in mice

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