Effects of prednisolone on behavioral and inflammatory profile in animal model of PTZ-induced seizure

Rosa, Gabriel de Lima; Guzzo, Edson Muller; Domingues, Amanda Muliterno; Padilha, Rafael Bremm; Amaral, Vitória Dias de Oliveira; Coitinho, Adriana Simon

doi:10.1016/j.neulet.2020.135560

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…É utilizado o PTZ, uma substancia que induz as crises convulsivas amplamente utilizado em modelos animais para investigações envolvendo crises convulsivas (Mussulini, 2013). O PTZ atua como um antagonista dos receptores GABAérgicos, bloqueando o influxo de íons de cloreto através desses canais e aumentando a excitabilidade neuronal e convulsões (de Lima Rosa et al, 2021).…”

Section: Introductionunclassified

“…Segundo de Lima Rosa et al, 2020 as doses de Prednisolona de 1mg/kg e 5mg/kg tiveram um efeito protetor nas crises, e o Diazepam levou a um efeito mais baixo nas crises de convulsão induzidas quando comparado com o grupo que recebeu a solução de salina a 0,9%. A Prednisolona teve um efeito protetor contra os níveis das citocinas inflamatórias IL-1β, IL-6 e TNF-α (de LimaRosa et al, 2021).No estudo de Oliveira 2008 com o Celecoxibe, os ratos foram divididos em quatro grupos onde foram submetidos a doses de 0,2mg/kg, 2mg/kg e 20mg/kg de Celecoxibe, no quarto grupo foi administrado doses de PGE2 via intraperitoneal. Os grupos receberam doses de 60mg/kg de PTZ após o tratamento com o medicamento e assim foram observadas as intensidades das crises convulsivas.…”

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Efeito dos anti-inflamatorios nas convulsões induzidas por pentilenotetrazol no modelo de Kindling: uma revisão

Barco

Linartevichi

2021

RSD

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Introdução: Estudos com anti-inflamatórios esteroidais e não esteroidais em modelo animal tem sido realizados a fim de verificar novas abordagens terapêuticas para o tratamento da epilepsia para melhorar a qualidade de vida dos pacientes portadores desta doença que acomete cerca de 65 milhões de pessoas no mundo. Objetivo: analisar a relevância no tratamento das crises convulsivas destes medicamentos. Metodologia: o presente estudo trata-se de uma revisão de literatura a fim de avaliar os efeitos dos anti-inflamatórios nas crises convulsivas. Resultado: os resultados obtidos demonstram que os animais tratados com os anti-inflamatórios esteroidais e não esteroidais estudados tiveram uma redução de intensidade nas crises convulsivas bem mais baixas sem evolução para a escala 4 de Racine. Conclusão: Conclui-se que os medicamentos anti-inflamatórios esteroidais e não esteroidais estudados tem o efeito protetivo e redutivo nas crises inflamatórias induzidas por pentilenotetrazol diminuindo as citocinas inflamatórias no sistema nervoso central.

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Section: Introductionunclassified

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Efeito dos anti-inflamatorios nas convulsões induzidas por pentilenotetrazol no modelo de Kindling: uma revisão

Barco

Linartevichi

2021

RSD

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“…In ammatory mediators induced by cytokines may be not only a complication of epilepsy, but also an internal inducement of some epilepsy diseases. For example, high levels of in ammatory mediators, including IL-1β, IL-6, and TNF-α were detected in the brain tissue of patients with intractable epilepsy (temporal lobe epilepsy caused by cortical dysplasia)(Bauer et al 2017;Elgarhi et al 2020;de Lima Rosa et al 2021). In our study, we found that PTZ induced generalized seizures and elevated IL-1β, IL-6, and TNF-α levels in kindled mice blood and brain.…”

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Chemical composition and anticonvulsant activities of herb pair of Gastrodia elata Blume-Acorus tatarinowii Schott decoction on experimentally induced seizures in mice

Yang

Yuan

et al. 2023

Metab Brain Dis

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Epilepsy is a serious public health problem in the world. At present, over 30% of affected patients remain refractory to currently available treatment. Medicinal plants as pharmaceuticals and healthcare treatments have been frequently used in the management of epilepsy in China for many centuries. Gastrodia elata-Acous tatarinowii (GEAT), as a classic and most commonly used herb pair in traditional Chinese medicine (TCM), has been employed to control seizures for thousands of years. However, the animal experiment data on its anticonvulsant effect is limited in the literature. Thus, this study aimed to reveal the therapeutic actions of GEAT decoction against seizures in mice. UHPLC-MS/MS was performed to analyze the chemical components of GEAT decoction.The mice were given GEAT decoction for 7 days, and MES, PTZ, and 3-MP injection was given 30 min after the last administration. Video monitoring was performed for comparisons. In addition, the PTZ-induced kindling models were conducted to investigate the seizure severity, anxiety and cognitive pro le, in ammation, and oxidative stress parameters in mice. The results showed that GEAT decoction dose-dependently protected mice against MES, 3-MP, and PTZ-induced acute seizures. Furthermore, GEAT decoction signi cantly ameliorated seizure severity, decreased the accumulation of in ammatory mediators TNF-α, IL-1β, and IL-6, mitigated oxidative stress, as well as alleviated anxious-like behavior and cognitive de cits in PTZ-kindled mice. These results suggest that GEAT decoction possesses certain anticonvulsant properties, which might be clinically useful as phytotherapy alone or as an adjunct therapy for the prevention and treatment of seizures and epilepsy.(model: Heidolph Hei-VAP). The concentrated solution was transferred to a glass bottle and then reserved at 4°C in the ice box. UHPLC-MS/MS analysis of GEAT decoctionUHPLC-MS/MS (Thermo Fisher Scienti c, USA) equipped with an electrospray ionization (ESI) source was applied for the qualitative analysis of phytochemical compounds from GEAT decoction. Chromatographic conditionChromatography was performed on a Zorbax Eclipse C 18 (1.8 µm×2.1 mm×100 mm). The mobile phase A consisted of 0.1% formic acid, and the mobile phase B was acetonitrile. Analysis accomplished by using a gradient elution of 5% B at 0-2 min, 5-30% B at 2-6 min, 30% B at 6-7 min, 30-78% B at 7-12 min, 78% B at 12-14 min, 78-95% B at 14-17 min, 95% B at 17-20 min, 95-5% B at 20-21 min, and 5% B at 21-25 min. The ow rate was 0.3 mL/min. The column temperature was set at 30°C. The injection volume of the sample was 2 µL. Mass spectrometry conditionThe Ion mode was set to positive and negative mode. MS conditions were: Spray voltages: 3.5 kV and -3.5 kV; Capillary temperature: 330°C; Sheath gas: 45 arbs; Aux gas: 15 arb and probe heater temperature: 325°C. Scan mode was full ms. Scanning mode: full scan (Full Scan, m/z 100 ~ 1500) and data-dependent mass spectrometry (dd-MS2, TopN = 10); resolution: 120,000 (MS1) & 60,000 (MS2). Collision Mode: High Energy...

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Saccharomyces Boulardii alleviates neuroinflammation and oxidative stress in PTZ-kindled seizure rat model

Mirzababaei,

Babaei,

Ghafghazi

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Effects of prednisolone on behavioral and inflammatory profile in animal model of PTZ-induced seizure

Cited by 9 publications

References 24 publications

Efeito dos anti-inflamatorios nas convulsões induzidas por pentilenotetrazol no modelo de Kindling: uma revisão

Efeito dos anti-inflamatorios nas convulsões induzidas por pentilenotetrazol no modelo de Kindling: uma revisão

Chemical composition and anticonvulsant activities of herb pair of Gastrodia elata Blume-Acorus tatarinowii Schott decoction on experimentally induced seizures in mice

Saccharomyces Boulardii alleviates neuroinflammation and oxidative stress in PTZ-kindled seizure rat model

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