Effects of Preconditioning and Temperature During Germination of 73 Natural Accessions of Arabidopsis thaliana

Schmuths, Heike; Bachmann, Konrad; Weber, Wilhelm; Horres, Ralf; Hoffmann, Matthias H.

doi:10.1093/aob/mcl012

Cited by 72 publications

(68 citation statements)

References 31 publications

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“…These results suggest that common genetic loci affected trait variation and that alleles had the same functional effects across treatments with the same temperature (in CL vs CS), whereas differences existed in the causal genetic loci or functional effects of alleles across treatments with different temperatures (in most comparisons involving WL). This significant temperature effect is in agreement with results of research on reproductive traits in other species (Jermstad et al, 2003;Hedhly et al, 2005;Schmuths et al, 2006). Also consistent with the finding that GEI was due to differential sensitivity to temperature, all but one QTL Â E interaction was in response to temperature.…”

Section: Discussionsupporting

confidence: 91%

The quantitative-genetic and QTL architecture of trait integration and modularity in Brassica rapa across simulated seasonal settings

Edwards

Weinig

2010

Heredity

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Within organisms, groups of traits with different functions are frequently modular, such that variation among modules is independent and variation within modules is tightly integrated, or correlated. Here, we investigated patterns of trait integration and modularity in Brassica rapa in response to three simulated seasonal temperature/photoperiod conditions. The goals of this research were to use trait correlations to understand patterns of trait integration and modularity within and among floral, vegetative and phenological traits of B. rapa in each of three treatments, to examine the QTL architecture underlying patterns of trait integration and modularity, and to quantify how variation in temperature and photoperiod affects the correlation structure and QTL architecture of traits. All floral organs of B. rapa were strongly correlated, and contrary to expectations, floral and vegetative traits were also correlated. Extensive QTL co-localization suggests that covariation of these traits is likely due to pleiotropy, although physically linked loci that independently affect individual traits cannot be ruled out. Across treatments, the structure of genotypic and QTL correlations was generally conserved. Any observed variation in genetic architecture arose from genotype Â environment interactions (GEIs) and attendant QTL Â E in response to temperature but not photoperiod.

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Section: Discussionsupporting

confidence: 91%

The quantitative-genetic and QTL architecture of trait integration and modularity in Brassica rapa across simulated seasonal settings

Edwards

Weinig

2010

Heredity

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“…Arabidopsis seed primary dormancy is promoted by low-temperature conditions during seed development (23)(24)(25). Low temperature also increases seed dormancy in wheat and this correlates with an increase in Ta-MFT gene expression (29).…”

Section: Resultsmentioning

confidence: 99%

“…Environmental conditions during seed set influence dormancy status. For example, low temperature conditions result in increased primary dormancy of mature Arabidopsis seeds (23)(24)(25) by inducing expression of genes associated with dormancy and influencing ABA and GA levels (24).…”

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confidence: 99%

Differential control of seed primary dormancy in Arabidopsis ecotypes by the transcription factor SPATULA

Vaistij

Gan

Penfield

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

111

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Freshly matured seeds exhibit primary dormancy, which prevents germination until environmental conditions are favorable. The establishment of dormancy occurs during seed development and involves both genetic and environmental factors that impact on the ratio of two antagonistic phytohormones: abscisic acid (ABA), which promotes dormancy, and gibberellic acid, which promotes germination. Although our understanding of dormancy breakage in mature seeds is well advanced, relatively little is known about the mechanisms involved in establishing dormancy during seed maturation. We previously showed that the SPATULA (SPT) transcription factor plays a key role in regulating seed germination. Here we investigate its role during seed development and find that, surprisingly, it has opposite roles in setting dormancy in Landsberg erecta and Columbia Arabidopsis ecotypes. We also find that SPT regulates expression of five transcription factor encoding genes: ABA-INSENSITIVE4 (ABI4) and ABI5, which mediate ABA signaling; REPRESSOR-OF-GA (RGA) and RGA-LIKE3 involved in gibberellic acid signaling; and MOTHER-OF-FT-AND-TFL1 (MFT ) that we show here promotes Arabidopsis seed dormancy. Although ABI4, RGA, and MFT are repressed by SPT, ABI5 and RGL3 are induced. Furthermore, we show that RGA, MFT, and ABI5 are direct targets of SPT in vivo. We present a model in which SPT drives two antagonistic "dormancy-repressing" and "dormancy-promoting" routes that operate simultaneously in freshly matured seeds. Each of these routes has different impacts and this in turn explains the opposite effect of SPT on seed dormancy of the two ecotypes analyzed here.phytohormone analyses | chromatin immunoprecipitation | transcriptomic analyses

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“…This makes the study of these processes in a model plant like Arabidopsis very relevant. Surprisingly, however, many studies in this species focus on the impact of elevated temperatures on germination (Baskin and Baskin, 1983;Schmuths et al, 2006;He et al, 2014;Silva-Correia et al, 2014), flowering time (Balasubramanian et al, 2006;Y. Li et al, 2014), and plant architecture (Wahid et al, 2007;Patel and Franklin, 2009;Antoun and Ouellet, 2013), but only very few address the impact of heat on fertility (Kim et al, 2001; (Warner and Erwin, 2005;Zinn et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Mapping of Fertility Reduction upon Heat Stress Reveals Developmental Stage-Specific QTLs in Arabidopsis thaliana

et al. 2015

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ORCID ID: 0000-0001-8918-0711 (J.J.B.K.)For crops that are grown for their fruits or seeds, elevated temperatures that occur during flowering and seed or fruit set have a stronger effect on yield than high temperatures during the vegetative stage. Even short-term exposure to heat can have a large impact on yield. In this study, we used Arabidopsis thaliana to study the effect of short-term heat exposure on flower and seed development. The impact of a single hot day (35°C) was determined in more than 250 natural accessions by measuring the lengths of the siliques along the main inflorescence. Two sensitive developmental stages were identified, one before anthesis, during male and female meiosis, and one after anthesis, during fertilization and early embryo development. In addition, we observed a correlation between flowering time and heat tolerance. Genome-wide association mapping revealed four quantitative trait loci (QTLs) strongly associated with the heat response. These QTLs were developmental stage specific, as different QTLs were detected before and after anthesis. For a number of QTLs, T-DNA insertion knockout lines could validate assigned candidate genes. Our findings show that the regulation of complex traits can be highly dependent on the developmental timing.

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Effects of Preconditioning and Temperature During Germination of 73 Natural Accessions of Arabidopsis thaliana

Abstract: Overall, the data show huge variation of germination and establishment among natural accessions of A. thaliana and might serve as a valuable source for further germination and plasticity studies.

Cited by 72 publications

References 31 publications

The quantitative-genetic and QTL architecture of trait integration and modularity in Brassica rapa across simulated seasonal settings

The quantitative-genetic and QTL architecture of trait integration and modularity in Brassica rapa across simulated seasonal settings

Differential control of seed primary dormancy in Arabidopsis ecotypes by the transcription factor SPATULA

Genome-Wide Association Mapping of Fertility Reduction upon Heat Stress Reveals Developmental Stage-Specific QTLs in Arabidopsis thaliana

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