PLoS ONE volume 9, issue 5, Pe98384 2014 DOI: 10.1371/journal.pone.0098384 View full text
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Guang-Li Zhang, Jun-Yu Ma, Quan Sun, Meng-Wen Hu, Xiu-yan Yang, Si-Hua Gao, Guang-Jian Jiang

Abstract: To study the time- and temperature-dependent survival of ovarian oocytes collected from postmortem carcass, ICR mice were killed and placed for different periods (0, 1, 2, 4, 6, 8 and 10 h) at different temperatures (25°C, 4°C and 37°C). After preservation, oocyte morphology, germinal vesicle (GV) oocyte number, oocyte meiotic maturation percentage, mitochondrial distribution and intracellular glutathione (GSH) level were evaluated. The results showed no surviving oocytes could be collected by 2h, 6h, and 12 h…

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