Effects of Polyunsaturated Fatty Acids in Growth Medium on Lipid Composition and on Physicochemical Surface Properties of Lactobacilli

Kankaanpää, Pasi; Yang, Baoru; Kallio, Heikki; Isolauri, Erika; Salminen, Seppo

doi:10.1128/aem.70.1.129-136.2004

Cited by 101 publications

(65 citation statements)

References 26 publications

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“…Jenkins and Courtney (2003) observed that linoleic acid inhibited the Lactobacillus growth but not conjugated linoleic acid. Kankaanpaa et al (2004) reported that the addition of polyunsaturated FAs such as linoleic, a-linolenic, arachidonic and docosahexaenoic acids to the growth medium affected the FAs composition of total lipids in Lactobacillus rhamnosus, L. casei and L. delbrueckii. However, effects of position, configuration and number of double bond in the FAs on the growth of Lactobacillus strains were poorly understood.…”

mentioning

confidence: 99%

Trans fatty acids promote the growth of some Lactobacillus strains

Endo

Kamisada

Fujimoto

et al. 2006

J. Gen. Appl. Microbiol.

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mentioning

confidence: 99%

Trans fatty acids promote the growth of some Lactobacillus strains

Endo

Kamisada

Fujimoto

et al. 2006

J. Gen. Appl. Microbiol.

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“…There are two groups of PUFA, ω-3 and ω-6. The effect of these two groups on lipid metabolism and the immune system differs (Stenbek, 1984;Vaughn and Reinhart, 1996;Calder, 1998;Kankaanpaa et al, 2004). The ω-3 PUFA exhibit anti-inflammatory and anti-proliferative influences on cells of the immune system.…”

mentioning

confidence: 99%

“…An increased supply of ω-3 PUFA decreases the level of triacylglycerols and cholesterol in blood plasma of experimental animals. The level of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids in plasma lipids increases at the expense of arachidonic acid (AA) (Herold and Kinsella, 1986;Fritsche et al, 1993;Das, 2002;Kankaanpaa et al, 2004) with a more pronounced effect of DHA compared to that of EPA (Morisaki et al, 1983). The mechanism of action of ω-3 PUFA on plasma lipids has not (Nestel, 1986) or the production of very low-density lipoproteins (VLDL) (Harris et al, 1984;Vaughn et al, 1994).…”

mentioning

confidence: 99%

The effect of probiotics potentiated with polyunsaturated fatty acids on the digestive tract of germ-free piglets

Kasteĺ¹,

Bomba²,

Vaško³

et al. 2007

Vet. Med. - Czech

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ABSTRACT:Oil with higher a content of ω-3 polyunsaturated fatty acids (PUFA) was administered for 4 weeks to experimental germ-free piglets and from 21 days of age the piglets were inoculated perorally with probiotic microorganisms Lactobacillus casei subsp. casei. Control piglets were administered saline solution in identical doses and starting from the fourth week were also supplemented with probiotics. At the age of 28 days, the number of Lactobacillus casei subsp. casei that had adhered to jejunal mucosa of experimental piglets was significantly higher (P < 0.05) compared to that in the control group. The differences in the number of lactobacilli, which colonised mucosa of the ileum and colon of experimental and control piglets, were insignificant. With the exception of the stomach, the pH level in the digestive tract of piglets was lower in the experimental piglets. Significantly higher levels of propionic (P < 0.05), acetoacetic and succinic (P < 0.01) acids were observed in germ-free experimental piglets in colon. The differences in the level of lactic and acetic acid were insignificant. PUFA-potentiated probiotics positively affected the adhesion of lactobacilli, pH and the level of organic acids in the digestive tract of germ-free piglets. Supplementation of oil containing ω-3 PUFA significantly increased the blood level of α-linolenic, eicosapentaenoic and docosahexaenoic acids in experimental piglets at the expense of arachidonic acid in comparison with the control.

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“…More importantly, growth in different media may entail physiological adaptations to a different nutrient composition that translate to the expression of different cell surface proteins and cell wall structure. 2 Thus, the objective of this study was to understand how growth in different growth medium affect the surface charge characteristics of Escherichia coli DH5α (ATCC 53868) through the binding of different proteins and metabolites to the cell surface as well as possible physiological adaptation of the cell envelope to growth in different growth medium.…”

Section: Introductionmentioning

confidence: 99%

Zeta potential of Escherichia coli DH5α grown in different growth media

2018

Preprint

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Graphical abstract Short descriptionCell surface charge of Escherichia coli DH5α (ATCC 53868), as measured by zeta potential, was found to vary with the type of growth medium used (e.g., LB Lennox, Tryptic Soy Broth, and formulated medium). Specifically, the general shape of the zeta potential-pH profile was the same irrespective of the growth medium used. Similar zeta potential-pH profile was obtained for growth of E. coli DH5α in LB Lennox, LB Lennox + 2 g/L glucose, and LB Lennox (buffered); thereby, highlighting that 2 g/L glucose supplementation, and a high capacity phosphate buffer did not result in physiological adaptation of the cell envelope. On the other hand, zeta potential-pH profiles of E. coli DH5α grown in Tryptic Soy Broth, LB Lennox (buffered) with 6 g/L glucose, formulated medium, and formulated medium with 6 g/L glucose were more negatively charged compared to that grown in LB Lennox medium. This suggested that there might be physiological adaptations to growth in different growth medium at the cell envelope level. Thus, considering that the cell 2 surface charge comprised intrinsic electrical charges of the cell wall and that from a nonspecifically adsorbed layer of ions and molecules, the zeta potential-pH profiles obtained revealed interesting information about how the electrostatic environment of the E. coli DH5α cell surface was perceived by other cells in the growth medium, which holds implications to our understanding of cell-cell interactions in the growth medium. AbstractProteins and metabolites typically adsorb to bacterial cell surface through a variety of mechanisms such as van der Waals attraction and electrostatic interactions, and forms a layer of nonspecifically adsorbed ions and molecules on the cell surface. Thus, the bacterial cell surface charge comprised the contribution from the cell wall as well as layers of nonspecifically adsorbed ions and molecules on the cell surface. This is the cell surface charge perceived by other bacterial cells in the growth medium. Given that different growth medium comprises different ensemble of proteins and metabolites that could adsorb onto the cell surface of bacteria, it is important to examine the effect of growth in different medium on the cell surface characteristics of bacterial cells using zeta potential as the proxy parameter. Defined at the shear plane, zeta potential provides a comprehensive view of the cell surface charge that include the nonspecifically adsorbed ions and molecules on the cell surface and the intrinsic electric charges in the cell wall. Using Escherichia coli DH5α (ATCC 53868) as model organism, experiments performed with deionized water as wash and resuspension buffer revealed that the zeta potential-pH profiles of cells grown in LB Lennox, and LB Lennox with 2 g/L glucose overlapped each other over the entire pH range from 2 to 9. This suggested that there was little physiological adaptation of the cell envelope of cells grown in LB Lennox supplemented with 2 g/L glucose, which indicated that the medium could ...

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Effects of Polyunsaturated Fatty Acids in Growth Medium on Lipid Composition and on Physicochemical Surface Properties of Lactobacilli

Cited by 101 publications

References 26 publications

Trans fatty acids promote the growth of some Lactobacillus strains

Trans fatty acids promote the growth of some Lactobacillus strains

The effect of probiotics potentiated with polyunsaturated fatty acids on the digestive tract of germ-free piglets

Zeta potential of Escherichia coli DH5α grown in different growth media

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