Trans fatty acids promote the growth of some Lactobacillus strains

Endo, Yasushi; Kamisada, Shusuke; Fujimoto, Kenshiro; Saito, Tadao

doi:10.2323/jgam.52.29

Cited by 32 publications

(30 citation statements)

References 15 publications

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“…Previous studies reported that oleic acid affected the cell growth (32) or survival in gastric juice (33) of some LAB strains by being incorporated into bacterial cell surface membrane lipids, but stearic acid did not. In this study, we found for the first time that adding decaglycerol mono-oleic acid ester Q-17S, normally used as an emulsifier for food, affected the immunomodulatory properties of L. plantarum strains, but decaglycerol monostearic acid ester Q-18S did not.…”

Section: Figmentioning

confidence: 99%

Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Toshimitsu

Ozaki

Mochizuki

et al. 2017

Appl Environ Microbiol

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Studies on the health-promoting effects of lactic acid bacteria (LAB) are numerous, but few provide examples of the relationship between LAB function and culture conditions. We verified the effect of differences in culture conditions on Lactobacillus plantarum OLL2712 functionality; this strain exhibits anti-inflammatory activity and preventive effects against metabolic disorders. We measured interleukin-10 (IL-10) and IL-12 production in murine immune cells treated with OLL2712 cells prepared under various culture conditions. The results showed that the IL-10-inducing activities of OLL2712 cells on murine immune cells differed dramatically between OLL2712 groups at different culture phases and using different culture medium components, temperatures, and neutralizing pHs. In particular, exponential-phase cells had much more IL-10-inducing activity than stationary-phase cells. We confirmed that the Toll-like receptor 2 (TLR2) stimulation activity of OLL2712 cells depended on culture conditions in conjunction with IL-10-inducing activity. We also demonstrated functional differences by culture phases in vivo; OLL2712 cells at exponential phase had more anti-inflammatory activity and anti-metabolic-disorder effects on obese and diabetic mice than those by their stationary-phase counterparts. These results suggest that culture conditions affect the functionality of antiinflammatory LAB.IMPORTANCE While previous studies demonstrated that culture conditions affected the immunomodulatory properties of lactic acid bacteria (LAB), few have comprehensively investigated the relationship between culture conditions and LAB functionality. In this study, we demonstrated several culture conditions of Lactobacillus plantarum OLL2712 for higher anti-inflammatory activity. We also showed that culture conditions concretely influenced the health-promoting functions of OLL2712 in vivo, particularly against metabolic disorders. Further, we characterized a novel mechanism by which changing LAB culture conditions affected immunomodulatory properties. Our results suggest that culture condition optimization is important for the production of LAB with anti-inflammatory activity.KEYWORDS culture phase, dendritic cell, interleukin-10, interleukin-12, Lactobacillus plantarum, macrophage, metabolic disorder, mono-oleic acid ester, proinflammatory cytokine, Toll-like receptor L actic acid bacteria (LAB) have been reported to modulate immune activities and prevent or ameliorate immune-related disorders, including inflammatory bowel diseases, allergies, and certain metabolic disorders (1-3). Certain LAB strains have been selected as the most suitable strains against each disorder (4-6). In addition to the

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Section: Figmentioning

confidence: 99%

Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Toshimitsu

Ozaki

Mochizuki

et al. 2017

Appl Environ Microbiol

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“…To develop probiotic cheeses, however, it is necessary to know the effect of all manufacturing steps and inputs on the survival of the added bacteria through the product shelf life. In this context, the use of CLA-enhanced milk might influence the viability of microorganisms in a probiotic cheese, as suggested by studies about the effects of specific fatty acids on lactobacilli growth and properties (Endo, Kamisada, Fujimoto, & Saito, 2006;Kankaanpää, Salminen, Isolauri, & Lee, 2001), and such effects should be evaluated.…”

Section: Introductionmentioning

confidence: 99%

Probiotic caprine Coalho cheese naturally enriched in conjugated linoleic acid as a vehicle for Lactobacillus acidophilus and beneficial fatty acids

Santos¹,

Bomfim²,

Vieira³

et al. 2012

International Dairy Journal

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“…One of these compounds is Tween 80 that is present Man Rogosa and Sharpe medium (De Man et al, 1960). Tween 80 is a water-soluble ester of oleic acid, which enhances growth and changes the fatty acid composition of cytoplasmic membrane of lactobacilli, making them resistant to environmental stresses (Endo et al, 2006;Li et al, 2011;Broadbent et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In this way, several studies were published relating the presence of Tween 80 in the culture medium to the membrane composition of lactobacilli (Nikkila et al, 1995;Endo et al, 2006;Broadbent et al, 2014). However, the effect of different concentrations of this surfactant on the metabolism of carbohydrates as well as the antibacterial activity of CFSs against MRSA and KPC strains are still under explored (Mariam et al, 2014;Arqués et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Activity of metabolites produced by new strains of Lactobacillus in modified de Man, Rogosa and Sharpe (MRS) medium against multidrug-resistant bacteria

Mesquita¹,

Costa²,

Frutuoso³

et al. 2017

Afr. J. Microbiol. Res.

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The emergence of nearly untreatable infections caused by multidrug-resistant bacteria has led to a new public health concern in which a need for development of alternative non-antibiotic strategies has become urgent. The activity of metabolites produced by new strains of Lactobacillus against multidrugresistant bacteria was investigated. The objective of this work was to isolate and identify lactobacilli from artisanal kefir by 16S rRNA gene sequencing as well as to evaluate the effect of the growth of Lactobacillus species in the Man Rogosa and Sharpe broth, supplemented with Tween 80 at concentrations of 0.6, 0.4, and 0.2 %. Cell Free Supernatants (CFSs) were obtained from these lactobacilli cultures and with them, organic acids (lactic acid and acetic acid) and ethanol were quantified by HPLC followed by the determination of their antimicrobial activities against eight strains of Staphylococcus aureus-MRSA and Klebsiella pneumonia KPC strains. The GenBank BLAST analysis revealed that, the isolated lactobacilli belonged to Lactobacillus paracasei (n=4) and Lactobacillus plantarum (n=5) strains. Increasing concentrations of Tween 80 did not affect the growth of Lactobacillus species significantly when compared to their controls (MRS broth). HPLC analysis of CFSs showed concentrations greater than 18.0, 4.0 and 1.0 g/L for lactic acid, acetic acid and ethanol, respectively. All CFSs were able to inhibit all pathogenic microorganisms evaluated. The percentage of inhibition was on average greater than 88% for MRSA and KPC strains. The antimicrobial activity was dependent on the CFSs tested. Based on these experimental conditions, organic acids and ethanol are likely to be responsible for this antimicrobial activity.

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Trans fatty acids promote the growth of some Lactobacillus strains

Cited by 32 publications

References 15 publications

Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Effects of Lactobacillus plantarum Strain OLL2712 Culture Conditions on the Anti-inflammatory Activities for Murine Immune Cells and Obese and Type 2 Diabetic Mice

Probiotic caprine Coalho cheese naturally enriched in conjugated linoleic acid as a vehicle for Lactobacillus acidophilus and beneficial fatty acids

Activity of metabolites produced by new strains of Lactobacillus in modified de Man, Rogosa and Sharpe (MRS) medium against multidrug-resistant bacteria

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