Effects of Polyunsaturated Fatty Acids and Clofibrate on Chicken Stearoyl-CoA Desaturase 1 Gene Expression

Lefèvre, Pascal; Tripon, E.; Plumelet, Céline; Douaire, Madeleine; Diot, Christian

doi:10.1006/bbrc.2000.4070

Cited by 29 publications

(20 citation statements)

References 37 publications

(24 reference statements)

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“…These results agree with those in this study as STAT5 was down-regulated and FASN up-regulated at the MED oil supplementation level. SREBP-1 contains a sterol regulatory element (SRE) and PUFA supplementation in rodents down regulates this transcription factor in rodents25 and chickens 26. Current research shows that FASN27 and SCD26 promoters contains recognition sites for SREBP-1, upstream stimulatory factor, stimulatory protein 1, and NF-Y, which are involved in the regulation by PUFA.…”

Section: Discussionmentioning

confidence: 99%

Omega-6 Fat Supplementation Alters Lipogenic Gene Expression in Bovine Subcutaneous Adipose Tissue

Joseph

Pratt

Paván

et al. 2010

Gene�Regul Syst Bio

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In contrast to rodents, adipose tissue serves as the major site of lipogenesis and storage reservoir for excess dietary energy in cattle. Research in rodents shows that adding corn oil (57% C18:2 n-6) to the diet alters lipogenesis enhancing deposition of omega-6 fatty acids. This study examines changes in lipogenic gene expression of subcutaneous adipose tissue from eighteen steers fed increasing levels of dietary corn oil [0 (NONE), 0.31 kg/d (MED) and 0.62 kg/d (HI)] using two platforms, qPCR and microarray. The results show that MED level of oil supplementation up-regulates gene expression of key lipogenic enzymes but that as oil supplementation reaches HI level mRNA encoding lipogenic enzymes responsible for de novo synthesis and desaturation are down-regulated. Changes in specific lipogenic mRNA levels are correlated with changes in tissue fatty acid composition where de novo and desatured fatty acids were reduced with the highest level of oil supplementation.

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Section: Discussionmentioning

confidence: 99%

Omega-6 Fat Supplementation Alters Lipogenic Gene Expression in Bovine Subcutaneous Adipose Tissue

Joseph

Pratt

Paván

et al. 2010

Gene�Regul Syst Bio

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“…1, 5 and 7). SCD1 gene transcription is stimulated by insulin to a similar level in LMH cells (Lefevre et al 2001) and mouse liver (Waters and Ntambi 1994) while enzyme activity in cultured avian liver explants is induced by 4 fold (Joshi and Aranda 1979). In our study, we showed that in hepatic cells, insulin induced SCD1 mRNA and protein expressions by more than 5 folds (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of insulin on SCD1 appears to be largely mediated by SREBP-1c (Shimomura et al 1998) however a direct binding of SREBP-1 on the SCD1 promoter has never been clearly associated to an insulin response (Tabor et al 1998(Tabor et al , 1999Waters et al 1997). In chicken LMH hepatoma cells, it was showed that insulin stimulates SCD1 gene expression through an Insulin Response Element (IRE) localized in the first 372 pb of the chicken promoter (Lefevre et al 2001).…”

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confidence: 99%

Role of the PI3-kinase/mTor pathway in the regulation of the stearoyl CoA desaturase (SCD1) gene expression by insulin in liver

Mauvoisin

Rocque

Arfa

et al. 2007

J. Cell Commun. Signal.

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The stearoyl-CoA desaturase 1 (SCD1) catalyzes the synthesis of monounsaturated fatty acids. This enzyme is a critical control point regulating hepatic lipogenesis and lipid oxidation. Therefore SCD1 may be a potential therapeutic target in the treatment of obesity and metabolic syndrome. Regulation of SCD1 expression occurs primarily at the level of transcription. In the present study, we characterized the insulin response elements (IREs) and the insulin signaling pathway mediating the regulation of SCD1 gene transcription in liver. In chicken embryo hepatocytes (CEH) and HepG2 cells, insulin stimulates SCD1 promoter activity by 2.5 folds. This activation is mediated by two different IREs on the chicken promoter, one localized between −1,975 and −1,610 bp and one between −372 and −297 bp. The latter binds both NF-Y and SREBP-1 transcription factors in response to insulin. We also demonstrated that insulin induction of SCD1 gene expression and promoter activity is abolished by pre-incubation of cells with specific inhibitors of both PI3-kinase (LY294002) and mTor (Rapamycin) or by over-expression of a dominant negative mutant of PI3-kinase. The PI3-kinase and mTor pathway mediates the insulin response on both IREs. In summary, insulin activates SCD1 gene expression in liver via a signaling pathway that involves PI3-kinase and mTor and the downstream transcription factors NF-Y and SREBP-1.Sentence summary: Insulin regulates SCD1 gene expression via two different IREs. The most 3′ IRE is localized between −372 and −297 bp and binds the NF-Y and SREBP-1 transcription factors in response to insulin. PI3-kinase and mTor mediate the action of insulin on both IREs.

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“…Human SCD-5 was abundantly expressed in brain and pancreas (Wang et al 2005). In birds, one SCD isoform (SCD) has been, so far, identified and cloned (GenBank accession number X60465) and very little is known about its tissue distributions and regulation (Lefevre et al 1999(Lefevre et al , 2001.…”

Section: Introductionmentioning

confidence: 99%

The regulation of stearoyl-CoA desaturase gene expression is tissue specific in chickens

Dridi¹,

Taouis²,

Gertler³

et al. 2007

Journal of Endocrinology

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Emerging evidence suggests a potential role of stearoyl-CoA desaturase (SCD)-1 in the control of body weight and energy homeostasis. The present study was conducted to investigate the effects of several energy balance-related factors (leptin, cerulenin, food deprivation, genotype, and gender) on SCD gene expression in chickens. In experiment 1, 6-week-old female and male broiler chickens were used. In experiment 2, two groups of 3-week-old broiler chickens were continuously infused with recombinant chicken leptin (8 mg/kg/h) or vehicle for 6 h. In experiment 3, two groups of 2-week-old broiler chickens received i.v. injections of cerulenin (15 mg/kg) or vehicle. In experiment 4, two broiler chicken lines (fat and lean) were submitted to two nutritional states (food deprivation for 16 or 24 h and feeding ad libitum). At the end of each experiment, tissues were collected for analyzing SCD gene expression. Data from experiment 1 showed that SCD is ubiquitously expressed in chicken tissues with highest levels in the proventriculus followed by the ovary, hypothalamus, kidney, liver, and adipose tissue in female, and hypothalamus, leg muscle, pancreas, liver, and adipose tissue in male. Female chickens exhibited significantly higher SCD mRNA levels in kidney, breast muscle, proventriculus, and intestine than male chickens. However, hypothalamic SCD gene expression was higher in male than in female (P!0 . 05). Leptin increased SCD gene expression in chicken liver (P!0 . 05), whereas cerulenin decreased SCD mRNA levels in muscle. Both leptin and cerulenin significantly reduced food intake (P!0 . 05). Food deprivation for either 16 or 24 h decreased the hepatic SCD gene expression in fat line and lean line chickens compared with their fed counterparts (P!0 . 05). The hypothalamic SCD mRNA levels were decreased in both lines only after 24 h of food deprivation (P!0 . 05). In conclusion, SCD is ubiquitously expressed in chickens and it is regulated by leptin, cerulenin, nutritional state, and gender in a tissue-specific manner.

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Effects of Polyunsaturated Fatty Acids and Clofibrate on Chicken Stearoyl-CoA Desaturase 1 Gene Expression

Cited by 29 publications

References 37 publications

Omega-6 Fat Supplementation Alters Lipogenic Gene Expression in Bovine Subcutaneous Adipose Tissue

Omega-6 Fat Supplementation Alters Lipogenic Gene Expression in Bovine Subcutaneous Adipose Tissue

Role of the PI3-kinase/mTor pathway in the regulation of the stearoyl CoA desaturase (SCD1) gene expression by insulin in liver

The regulation of stearoyl-CoA desaturase gene expression is tissue specific in chickens

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