Effects of polystyrene nanoplastics on Ctenopharyngodon idella (grass carp) after individual and combined exposure with zinc oxide nanoparticles

Estrela, Fernanda Neves; Guimarães, Abraão Tiago Batista; Silva, Fabiano Guimarães; Luz, Thiarlen Marinho da; Silva, Abner Marcelino; Pereira, Paulo Sérgio; Malafaia, Guilherme

doi:10.1016/j.jhazmat.2020.123879

Cited by 77 publications

(15 citation statements)

References 103 publications

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“…While catalase activity was assessed based on Sinha et al (1972) andMontalvão et al (2021). The SOD was determined according to the method described by Del-Maestro & McDonald (1985) (initially) and adapted by Estrela et al (2021). To assess the balance between the synthesis of hydrogen peroxide by SOD and its decomposition by catalase, the SOD/CAT ratio was calculated and recorded, as Liu et al (2017).…”

Section: Methodsmentioning

confidence: 99%

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An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

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The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpoles / 5 replicates of 10 animals) from species Physalaemus cuvieri (Leptodactylidae). After synthesis, purification, and characterization of peptides (PSDP2001, PSDP2002, PSDP2003) an aquatic contamination has been simulatedwith these peptides during 24 hours of exposure in two concentrations (100 and 500 ng/mL). The control group (“C”) was composed of tadpoles kept in polyethylene containers containing de-chlorinated water. Oxidative stress, antioxidant biomarkers and neurotoxicity activity were assessed. In both concentrations, PSPD2002 and PSPD2003 increased catalase and superoxide dismutase antioxidants enzymes activities, as well as oxidative stress (nitrite levels, hydrogen peroxide and reactive oxygen species). All three peptides also increased acetylcholinesterase activity in the highest concentration. These peptides showed molecular interactions in silico with acetylcholinesterase and antioxidant enzymes. Aquatic particle contamination of SARS-CoV-2 has neurotoxics effects in P. cuvieri tadpoles. These findings indicate that the COVID-19 can constitute environmental impact or biological damage potential.HIGHLIGHTSSARS-CoV-2 spike protein peptides (PSDP) were synthesized, purified, and characterized by solid phase peptide synthesis.PSDP peptides promoted REDOX imbalance and acute neurotoxicity in tadpoles (Physalaemus cuvieri)In silico studies have shown interactionsbetween peptides and acetylcholinesterase and antioxidant enzymesAquatic particle contamination of SARS-CoV-2 can constitute additional environmental damageGRAPHICAL ABSTRACT

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Section: Methodsmentioning

confidence: 99%

“…Peptide neurotoxicity was assessed by quantifying acetylcholinesterase (AChE) activity, based on the method by Ellman et al (1961), with minor detailed modifications in Estrela et al (2021). AChE activity is used as a cholinergic function marker since it regulates the acetylcholine (ACh) amount interacting with its receptors (Tougu, 2001).…”

Section: Methodsmentioning

confidence: 99%

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

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“…Different pollutants can affect the nutritional status of tadpoles (Bharatraj & Yathapu, 2018); therefore, we evaluated total soluble carbohydrate, total protein, and triglyceride concentrations in different tissues of the exposed animals. Total soluble carbohydrate levels were determined through the Dubois method (Dubois et al, 1956) - detailed by Estrela et al (2021). Protein level was determined in commercial kit (Bioténica Ind.…”

Section: Methodsmentioning

confidence: 99%

“…These enzymes are considered first-line antioxidants important for defense strategies against oxidative stress (Ighodaro & Akinloye, 2018). Catalase activity was assessed based on Sinha et al (1972) [see details in Montalvão et al (2021)] and SOD was determined according to the method originally described by Del-Maestro & McDonald (1985) and adapted by Estrela et al (2021).…”

Section: Methodsmentioning

confidence: 99%

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Guimarães

Estrela

Rodrigues

et al. 2021

Preprint

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Although carbon nanotubes (CNTs) toxicity in different experimental systems (in vivo and in vitro) is known, little is known about the toxic effects of carbon nanofibers (CNFs) on aquatic vertebrates. We herein investigated the potential impact of CNFs (1 and 10 mg/L) by using Physalaemus cuvieri tadpoles as experimental model. CNFs were able to induce nutritional deficit in animals after 48-h exposure to them, and this finding was inferred by reductions observed in body concentrations of total soluble carbohydrates, total proteins, and triglycerides. The increased production of hydrogen peroxide, reactive oxygen species and thiobarbituric acid reactive substances in tadpoles exposed to CNFs has suggested REDOX homeostasis change into oxidative stress. This process was correlated to the largest number of apoptotic and necrotic cells in the blood of these animals. On the other hand, the increased superoxide dismutase and catalase activity has suggested that the antioxidant system of animals exposed to CNFs was not enough to maintain REDOX balance. In addition, CNFs induced increase in acetylcholinesterase and butyrylcholinesterase activity, as well as changes in the number of neuromats evaluated on body surface (which is indicative of the neurotoxic effect of nanomaterials on the assessed model system). To the best of our knowledge, this is the first report on the impact of CNFs on amphibians; therefore, it broadened our understanding about ecotoxicological risks associated with their dispersion in freshwater ecosystems and possible contribution to the decline in the populations of anurofauna species.

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“…Genotoxicity, as a vital component of a comprehensive toxicological profile, has been affirmed to be mechanistically connected with numerous adverse health consequences, such as neurodegenerative disorders and birth defects (Turkez et al, 2017;Hsieh et al, 2019). Recently, many researchers have performed the genotoxicity assays of MPs on aquatic organisms at environmentally relevant concentrations (Brandts et al, 2018;González-Soto et al, 2019;Revel et al, 2019;Revel et al, 2020;Estrela et al, 2021). However, there were divergent findings documented.…”

Section: Introductionmentioning

confidence: 99%

Evidence-based meta-analysis of the genotoxicity induced by microplastics in aquatic organisms at environmentally relevant concentrations

Sun

Zhan

et al. 2021

Science of The Total Environment

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Effects of polystyrene nanoplastics on Ctenopharyngodon idella (grass carp) after individual and combined exposure with zinc oxide nanoparticles

Cited by 77 publications

References 103 publications

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Evidence-based meta-analysis of the genotoxicity induced by microplastics in aquatic organisms at environmentally relevant concentrations

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