Effects of polystyrene in the brackish water flea Diaphanosoma celebensis: Size-dependent acute toxicity, ingestion, egestion, and antioxidant response

Yoo, Ju-Soon; Cho, Hayoung; Jeon, MinJeong; Jeong, Chang‐Bum; Jung, Jee-Hyun; Lee, Young-Mi

doi:10.1016/j.aquatox.2021.105821

Cited by 39 publications

(11 citation statements)

References 38 publications

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“…showed the most signi cant changes under chemical exposure conditions in our previous studies 42,45,46,47 . The fold change values were calculated using the 2 △△Ct method 54,55 using formulas (1) and ( 2…”

Section: Competing Interestsmentioning

confidence: 56%

“…Furthermore, the results of studies on reference genes have been helpful in using these species in ecotoxicological studies, followed by the suggestion of speci c genes 39,40 . In the brackish water ea, D. celebensis is also one of the potential species that might be used in several studies on pollution in many tropical and coastal areas, either in lab-based work or elds 25,41,42 . The nine candidate reference genes, Atb, Act, 18S, GAPDH, EF-1b, UBC, H2A, TBP, and SDH were used to identify adequate and reliable reference genes for brackish water ea exposed to waterborne chemicals.…”

Section: Discussionmentioning

confidence: 99%

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Validation of Reference Genes for Quantitative Real-time PCR in Diaphanosoma Celebensis: For Chemical Exposure Conditions and Different Ages

Lee¹,

In²,

Kim³

et al. 2021

Preprint

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Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), a primary approach for evaluating gene expression, requires an appropriate normalization strategy to rule out variations in gene expression among samples. The best option is to use a reference gene whose expression level is stable across various experimental conditions to compare the mRNA levels of a target gene. However, there is limited information on how the reference gene is differentially expressed at different ages (growth) in small invertebrates with notable changes such as molting. In this study, expression profiles of nine candidate reference genes from the brackish water flea, Diaphanosoma celebensis, were evaluated under diverse exposure to toxicants and according to growth. As a result, four different algorithms showed similar stabilities of genes for chemical exposures in the case of limited conditions using the same developmental stage (e.g., adult), while the results according to age showed a significantly different pattern in suite of candidate reference genes. This affected the results of genes EcRA and GST, which are involved in development and detoxification mechanisms, respectively. Our finding is the first step towards establishing a standardized real-time qRT-PCR analysis of this environmentally important invertebrate that has potential for aquatic ecotoxicology, particularly in estuarine environments.

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Section: Competing Interestsmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Validation of Reference Genes for Quantitative Real-time PCR in Diaphanosoma Celebensis: For Chemical Exposure Conditions and Different Ages

Lee¹,

In²,

Kim³

et al. 2021

Preprint

Self Cite

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show abstract

“…When investigating the effect of PS on the brackish water flea Diaphanosoma celebensis , a size-dependent oxidative stress response was found. The smaller PS NPs had a longer retention time and higher acute toxicity than micro-sized ones ( Yoo et al., 2021 ). Ma et al.…”

Section: Eco-impacts Of Nps Are Higher Than Those Of Mpsmentioning

confidence: 99%

Nanoplastics are significantly different from microplastics in urban waters

et al. 2023

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“…Three-hundred nanometers of PS induced higher CAT and GPx activities in D. magna than the six-hundred-nanometer ones [ 121 ]. Additionally, 0.05 and 0.5 μm PS significantly increased the MDA concentration in Diaphanosoma celebensis while 6 μm PS caused moderate alterations [ 122 ]. Plain PS generated greater oxidative stress in D. magna than PS-COOH and PS-

[ 50 ], while amine-modified PS and carboxyl-modified PS both caused more severe oxidative damage to D. magna than PS [ 123 ].…”

Section: Effects Of Microplastics On Physiological Functions Of Aquat...mentioning

confidence: 99%

“…Additionally, for nanoplastics, nano-sized PS induced significant oxidative stress to aquatic crustaceans by penetrating the cellular membrane, causing more cellular damage than larger microplastics. These effects could be shown by the transcriptional modulation of antioxidant-related genes and enzyme activities [ 122 ]. Nano-sized PS induced D. pulex oxidative stress, affecting the arachidonic acid metabolism, glutathione metabolism and porphyrin and chlorophyll metabolism [ 145 ].…”

Section: Response Mechanisms Of Aquatic Crustaceans To Microplastics ...mentioning

confidence: 99%

Progress on the Effects of Microplastics on Aquatic Crustaceans: A Review

Zhang

Hou

2023

IJMS

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It is impossible to overlook the effects of microplastics on aquatic life as they continuously accumulate in aquatic environments. Aquatic crustaceans, as both predator and prey, play an important role in the food web and energy transmission. It is of great practical significance to pay attention to the toxic effects of microplastics on aquatic crustaceans. This review finds that most studies have shown that microplastics negatively affect the life history, behaviors and physiological functions of aquatic crustaceans under experimental conditions. The effects of microplastics of different sizes, shapes or types on aquatic crustaceans are different. Generally, smaller microplastics have more negative effects on aquatic crustaceans. Irregular microplastics have more negative effects on aquatic crustaceans than regular microplastics. When microplastics co-exist with other contaminants, they have a greater negative impact on aquatic crustaceans than single contaminants. This review contributes to rapidly understanding the effects of microplastics on aquatic crustaceans, providing a basic framework for the ecological threat of microplastics to aquatic crustaceans.

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Effects of polystyrene in the brackish water flea Diaphanosoma celebensis: Size-dependent acute toxicity, ingestion, egestion, and antioxidant response

Cited by 39 publications

References 38 publications

Validation of Reference Genes for Quantitative Real-time PCR in Diaphanosoma Celebensis: For Chemical Exposure Conditions and Different Ages

Validation of Reference Genes for Quantitative Real-time PCR in Diaphanosoma Celebensis: For Chemical Exposure Conditions and Different Ages

Nanoplastics are significantly different from microplastics in urban waters

Progress on the Effects of Microplastics on Aquatic Crustaceans: A Review

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