Effects of polymorphisms at <i>LEP</i>, <i>CAST</i>, <i>CAPN1</i>, <i>GHR</i>, <i>FABP4</i> and <i>DGAT1</i> genes on fattening performance and carcass traits in Simmental bulls

Abstract

Abstract. The aim of this study was to investigate the effects of single nucleotide polymorphisms (SNPs) at six candidate genes (LEP, CAST, CAPN1, GHR, FABP4 and DGAT1) on fattening performance and carcass traits of Simmental bulls in Turkey. The analysis covered a total of 81 Simmental bulls grown on a private farm that were randomly selected for their fattening period for use in this study. Genotyping was performed using the PCR-RFLP method. The S20T polymorphism at the CAST gene and the G316A polymorphism at the CAPN1 gene were associated with variation in final weight, fattening period, weight gain and average daily gain (P < 0.05). In addition, LEP A80V had a significant effect on hot and chilled carcass weight and dressing percentage (P < 0.05). There was no association between GHR S555G, FABP4 V110M and DGAT1 K232A markers with the traits analysed. These results suggested that focusing on the novel effects of LEP, CAST and CAPN1 gene polymorphisms on meat production traits might be useful for marker-assisted selection in Simmental cattle.

Individual and combined effects of <i>CAPN1</i>, <i>CAST</i>, <i>LEP</i> and <i>GHR</i> gene polymorphisms on carcass characteristics and meat quality in Holstein bulls

“…In addition, Pintos and Corva (2011) found significant associations between the same marker with birth weight, weaning weight and live weight recorded at 18 months of age in Angus cattle. Ardicli et al (2017b) reported that homozygous animals for allele G at the CAPN1 G316A marker reached the highest final weight and total weight gain in a shorter fattening period with higher average daily gain in Simmental bulls. Among the factors considered, it is possible that Warner-Bratzler shear force (WBSF) values show an association with LW and that selection for this marker may lead to changes in both traits.…”

The impact of diacylglycerol O-acyltransferase 1 gene polymorphism on carcass traits in cattle

“…Due to the prevalence of homozygous AA animals (85.71%), the superiority of A allele across animals in population was found (A=0.93, K=0.07). Such a low frequency of K allele was reported for various breeds of beef or dual-purpose cattle, including Charolais (Ripoli et al, 2006), Angus (Kaupe et al, 2007), Simmental (Ardicli et al, 2017), and Russian beef cattle (Gorlov et al, 2014). On the other hand, the dairy cattle (Holstein, Jersey) showed much higher frequency of K allele (Kaupe et al, 2004;Casas et al, 2005;Citek et al, 2007).…”

“…Carcass traits are alongside fattening performance one of the most important traits in beef industry. It is generally accepted that the beef production is very complex and time-consuming field of the livestock industry (Gorlov et al, 2014;Ardicli et al, 2017). The majority part of traits associated with carcass quality and meat yield is under the control of polygenic inheritance.…”

“…The diacylglycerol O-acyltransferase 1 (DGAT1) gene (GenBank no. AY065621) encodes a microbial enzyme catalysing the last step of the synthesis of triglycerides (Ardicli et al, 2017). During the metabolism of fatty acid, the DGAT1 participates in the conversation of carbohydrates into fat and their storage in the fat depot (Gorlov et al, 2014).…”

“…The most studied SNP K232A is caused by dinucleotide substitution of ApA for GpC (AAG→GCG) in position 10433 and 10434 at the beginning of exon 8 resulting in non-conservative substitution of lysine for alanine (Winter et al, 2002;Sedykh et al, 2017). Many studies demonstrated significant impact of K232A polymorphism on milk production (Citek et al, 2007;Gautier et al, 2007;Cerit et al, 2014;Bekseitov et al, 2017) and meat production traits (Yuan et al, 2013;Tait et al, 2014;Ardicli et al, 2017) in various cattle breeds. The objective of this study was to determine the genetic structure of population based on K232A polymorphism in DGAT1 gene and to analyse its impact on carcass traits in Slovak Pinzgau cattle.…”