The quality of 40 carcasses of light lambs of the synthetic population of Slovak Dairy sheep from (a) artificial rearing (AR) and (b) traditional rearing (TR) was assessed on the basis of fatty acids profile of intramuscular fat (IMF). Lambs from AR in comparison with TR were of lower quality as assessed on the basis of fatty acids (FAs) profile. The content of conjugated linolic acid (CLA) in the fat of TR lambs was severalfold higher (0.749 vs. 0.193 g.100g -1 FAME, P<0.001) than in AR lambs. Similarly, the content of trans-vaccenic (TVA), α-linolenic (ALA), rumenic (RA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) was in TR lambs significantly higher (P<0.001) than in AR lambs (0. -1 FAME). In contrary, the content of linoleic acid (LA), the ratio of LA/ALA and n-6/n-3 in AR lambs was higher than in TR lambs (9.07 vs. 4.81 g.100 g -1 FAME; 39.11 vs. 6.80; 14.56 vs. 3.25, P<0.001). In TR lambs the content of n-3 PUFA and BCFA was significantly higher (P<0.001) than in AR lambs (2.08 vs. 0.84 and 1.95 vs. 0.45). The value of thrombogenic index was higher in AR lambs in comparison with TR lambs (1.44 vs. 1.31; P<0.05). Significant differences between FAs of IMF of ram lambs and ewe lambs were observed only in the case of arachidonic acid (P<0.05).Keywords: sheep, light lambs, artificial and traditional rearing, carcass, meat quality, intramuscular fat, fatty acids, CLA
AbstraktCieľm práce bolo posúdiť kvalitu 40 ľahkých jatočných jahniat syntetickej populácie slovenskej dojnej ovce z (a) tradičného odchovu (TO) a (b) umelého odchovu (UO) na základe stanovenia obsahu mastných v intramuskulárnom tuku najdlhšieho chrbtového svalu. Jatočné jahňatá z UO nedosahovali kvalitu jahniat z TO. Obsah konjugovanej kyseliny linolovej (CLA) bol štatisticky vysoko významne (P<0,001)
The objective of this study was to apply high resolution melting analysis in the detection of complex vertebral malformation (CVM) carriers in Hosltein cattle. A total of 47 animals of Holstein cattle were included in this study. Genomic DNA was extracted from blood, hair follicles and sperm by commercial QIAamp® DNA Mini kit. The amplification and high resolution melting analysis (HRMA) was done by commercial SensiMixTM HRM kit. The confirmation of sensitivity of this method was done by PCR-PIRA method and sequencing. Four samples of heterozygous genotype GT for causal mutation in the bovine solute carrier family 35 member 3 gene (SLC35A3), which is responsible for CVM disease, were detected. Our results demostrated that the use of HRMA for genotyping of mutant allele T for SLC35A3 gene in Holstein cattle is an effective method for the selection of carriers of CVM disease
The aim of this study was to identify the K232A polymorphism in gene encoding diacylglycerol O-acyltransferase 1 (DGAT1) and to evaluate its effect on carcass traits in population of Slovak Pinzgau steers. The genotyping data were obtained for totally 56 animals by using PCR-RFLP method. The A allele (0.93) was more frequent in analysed population than K allele (0.07). Even if the expected and observed heterozygosity indicated prevalence of homozygotes, the significant effect of inbreeding on population structure wasn't found (FIS=-0.08). The decline in effective allele number (Na=1.15) as well as polymorphic information content (PIC=0.12) pointed out to significant decrease of locus effectiveness in population. The effect of DGAT1 gene polymorphism on carcass traits was tested by using the GLM procedure adopted in SAS 9.3. In association analysis the proportion of muscle, fat, bone, and drip loss within the beef three-rib section were evaluated. However, the statistical analysis showed only non-significant impact of DGAT1 gene polymorphism on selected production traits in analysed population.
The aim of study was to evaluate the effect of intramuscular fat content on physical parameters and proximate composition in musculus longissimus dorsi (MLD) from Mangalitsa breed and Slovak Large White x Mangalitsa crossbreed. In the study, sixteen pigs of Mangalitsa and twenty-two pigs of Slovak Large White x Mangalitsa crossbreed were used. The pigs were reared under intensive condition and all animals were fed ad libitum with complete fattening feed mixture. The fattening period started from 30 kg of live weight. Then the pigs were slaughtered at 100 kg of live weight. Chemical analysis showed that MLD from Mangalitsa had lower protein content, higher moisture content (p <0.05) and higher content of intramuscular fat compared to Slovak Large White x Mangalitsa crossbreed. As regarding the cholesterol content in MLD, no significant differences were found between genotypes, but the cholesterol content was higher in MLD from Mangalitsa than in MLD from crossbreeds. The MLD from Mangalitsa exhibited lower CIE L* (p <0.01) and CIE b* (p <0.01) values 45 min post mortem compared to crossbreeds. Colour parameters increased after 7 days post mortem, which is normal due to the maturing process of the meat. Then the CIE L* value was lower in MLD from Mangalitsa (p <0.01), but CIE a* value was higher in relation to crossbreeds (p <0.05). As regards the Warner-Bratzler shear force, the meat from Mangalitsa was tenderer than in crossbreeds (p <0.05). Intramuscular fat in the meat positively correlated with colour parameter CIE a* (r = 0.324; p <0.05) as well as cholesterol content (r = 0,656; p <0.001). In contrast in the study was found negative correlations between intramuscular fat in meat with moisture content (r = -0.399; p <0.05) and protein content (r = -0.812; p <0.001). It can be concluded that the percentage of intramuscular fat significantly influenced the physical and the chemical parameters of pork. The meat from Mangalitsa is more suitable for production of special meat products (fermented and smoked).
The present experiment was aimed to study the effect of bee pollen on the meat chemical composition of broiler’s Ross 308 breast and thigh muscles. In the experimental groups were added bee pollen in an amount (group E1 – 2 500 mg.kg−1, group E2 – 3 500 mg.kg−1 and E3 – 4 500 mg.kg−1) to the feed mixtures for 42 days. At the end of the study the water content was higher in experimental groups than the control group and on breasts there were a significant difference (P ≤ 0.05) between control groups and experimental groups (E1, E2 and E3) also there was a significant difference (P ≤ 0.05) between the experimental E3 and experimental E1, E2 groups. In protein content, the control group was higher than experimental groups and there no significant differences (P ≥ 0.05) among the groups. In fat content, the control group (2.04%, 13.2%) was higher than experimental groups E1 group (1.59%, except thigh 14.11%), E2 group (1.70%, 13.00%) and E3 group (1.51%, 10.96%) and on breast there were significant differences (P ≤ 0.05) between control group and experimental groups E1, E3 and on thigh there were significant differences (P ≤ 0.05) between experimental E3 and experimental E1, E2 groups. In energy value (kJ.100 g−1) of the breast and thigh muscles in control was higher than experimental groups and on the breasts there were a significant differences (P ≤ 0.05) between control group and experimental groups (E1, E3) and on the thigh there were significant differences (P ≤ 0.05) between experimental E1 and experimental E3 groups. From the study we concluded that the bee pollen has a positive effect of the breast’s meat chemical composition of broiler, which led to increase the water contact and reduce the fat content and energy value, but he had a normal effect on thigh, also bee pollen has normal effect on the protein content of the breast and thigh muscles.
