Effects of pleuropneumonia-like organisms on cultured human cells

Fogh, Jørgen; Hahn, Edwin C.; Fogh, H

doi:10.1016/0014-4827(65)90057-1

Cited by 29 publications

(10 citation statements)

References 13 publications

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“…It has been identified as a strain of mycoplasma fermentans (PG-18) by Dr. M. Barile, Division of Biologics Standards, National Institutes of Health, Dr. J. G. Tully, Laboratory of Bacterial Diseases, National Institutes of Allergy and Infectious Diseases, and Dr. Donald Armstrong, Sloan-Kettering Institute. The mycoplasma strain received many tissue culture passages as an experimental contaminant of FL cells and the derived, modified cell lines (5)(6)(7)(8)(9). For infection of amnion cultures in the present experiments, the mycoplasma was subcultured several times in BYE broth (10) to prevent the introduction of foreign cell types into the experimental cultures.…”

Section: Materials and Methods Virus The Sv40mentioning

confidence: 99%

“…Mycoplasma, strain HT (5) , was originally isolated .its a tissue culture contaminant. It has been identified as a strain of mycoplasma fermentans (PG-18) by Dr. M. Barile, Division of Biologics Standards, National Institutes of Health, Dr. J. G. Tully, Laboratory of Bacterial Diseases, National Institutes of Allergy and Infectious Diseases, and Dr. Donald Armstrong, Sloan-Kettering Institute.…”

Section: Materials and Methods Virus The Sv40mentioning

confidence: 99%

“…Presumably, the results can best be interpreted as a combination of effects. The H T strain of mycoplasma is cytopathic for SV4B transformed amnion cells [pronounced destruction in strain H (Mli)], as for FL cells (5) and other spontaneously transformed cells, whereas primary amnion cells are relatively resistant. Lack of transformation, late appearance of transformed foci, and lesser growth potential of the strains resulting from concurrent infection with SV40 and mycoplasma may, therefore, in part, be explained by the mycoplasma's cell destructive effects, both initially and throughout subculture.…”

Section: As Shown Inmentioning

confidence: 99%

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Mycoplasma Effects on SV40 Transformation of Human Amnion Cells

Fogh

1970

Experimental Biology and Medicine

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The reproducible SV40 transformation of human epithelial amnion cells in vitro and the characteristics of the strains of transformed cells during subcultivation, have been reported previously (1-4). Since we have established that mycoplasma infection may affect spontaneously transformed amnion cells of the FL line, causing changes in morphology and growth ( 5 ) , in chromosome numbers and abnormalities (6-8), 2nd in tumor-producing capacity (9), it seemed pertinent to investigate possible effects of mycoplasma on SV40 transformation of amnion cells.The present data demonstrate that concurrent SV40 and mycoplasma infection modified the SV40 transformation. Effects were noticeable during the early events of transformation, and the characteristics of the transformed amnion strains were also affected. Materials and Methods. Virus. The SV40strain VA 45-54 GMK 4 used in the present experiments, has been previously described ( 3 ) . Details of methods for determining the infectious unit, IDSO/ml of culture fluid, using African green monkey kidney cells, have also been reported ( 3 ) . Since samples for virus titration contained mycoplasma which propagated to high titers in the test cultures, it was determined if the presence of mycoplasma had any effects on the virus titers. A series of SV40 and mycoplasma infected culture fluids were collected, and mycoplasma was eliminated from one aliquot of each by treatment with chlortetracycline HCI ( Aureomycin Lederle) , 1000 pg/ml for 5 hr at 37". These and the untreated aliquots were serially diluted for comparative end-1 This investigation was supported in part by point titrations in cultures of the monkey kidney cells. In the range of virus titers tested ( 104-5-107.5), the end points remained the same regartdless of the presence or absence of mycoplasma. However, the time of appearance of maxium cytopathic effect was delayed several days when. mycoplasma was present, and the morphological changes differed from those observed in the absence of mycoplasma. Thus, the vacuolization of the cells was less pronounced, and the cell sheet was held together by a stranded matrix. These differences were taken into consideration in the following experimental virus titrations.

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Section: Materials and Methods Virus The Sv40mentioning

confidence: 99%

Section: Materials and Methods Virus The Sv40mentioning

confidence: 99%

Section: As Shown Inmentioning

confidence: 99%

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Mycoplasma Effects on SV40 Transformation of Human Amnion Cells

Fogh

1970

Experimental Biology and Medicine

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“…The origin of mycoplasma strain HT (8) and its identification have also been reported ( 9 ) , as have methods for de-tection, elimination, and quantitative determinations .of colony-forming units for this strain ( 2 ) .…”

mentioning

confidence: 98%