Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro

Abstract

BackgroundEndometritis reduces fertility and is responsible for major economic losses in beef and dairy industries. The aim of this study was to evaluate an alternative therapy using platelet-rich plasma (PRP). PRP was tested in vivo, after bovine intrauterine administration, and in vitro on endometrial cells.MethodsBovine endometrial cells were cultured until passage (P) 10 with 5 % or 10 % PRP. Effect of PRP on endometrial cell proliferation and on the expression of genes [prostaglandin-endoperoxide synthase 2 (COX2), tumor protein p53 (TP53), oestrogen receptors (ER-α and ER-β), progesterone receptor (PR) and c-Myc] involved in the regulation of oestrus cycle and fetal-maternal interaction were evaluated. Moreover, to evaluate the ability of PRP to counteract inflammation, 10 and 100 ng/ml of bacterial endotoxin lipopolysaccharide (LPS) were used to inflame endometrial cells in vitro for 1, 6, 12, 24 and 48 h. The expression of genes such as interleukin 1β (IL-1β), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), prostaglandin-endoperoxide synthase 2 (COX2/PTGS2), and the release of PGE-2, IL-1β and IL-8 were evaluated.ResultsIn vivo treatment with PRP increased the detection of PR. In vitro, 5 % PRP at passage 5 increased proliferation rate and induced a significant increase in the expression of all studied genes. Furthermore, the results revealed that 10 ng/ml of LPS is the most effective dose to obtain an inflammatory response, and that PRP treatment significantly down regulated the expression of pro-inflammatory genes.ConclusionThis study lays the foundations for the potential treatment of endometritis with PRP in vivo.

Successful Implantation and Live Birth Following Autologous Platelet-rich Plasma Treatment for a Patient with Recurrent Implantation Failure and Chronic Endometritis

“…The underlying mechanisms through which PRP acts as a modulator alleviating inflammation are further strengthened by in vitro studies of PRP treatment on animal models (20,45). Proliferation as well as gene expression of endometrial cells have already been demonstrated and confirmed by in vitro bovine studies.…”

“…c-MYC is involved in cell in vivo 33: 515-521 (2019) proliferation and growth, and is activated by EGF, a component of PRP. This is one representative of many examples of the effects of PRP, demonstrating its effectiveness in reducing gene expression of pro-inflammatory factors such as interleukin 1β, interleukin 8, prostaglandin-endoperoxide synthase 2, and inducible nitric oxide synthetase, revealing its promising prospectives in in vivo regenerative therapy in endometritis (45). El-Sharkawy et al reported that treatment with PRP increased the release of various growth factors such as insulin-like growth factor-I, fibroblast growth factor, epidermal growth factor and transforming factor β (46).…”

“…Although, the molecular mechanisms still belong in the grey zone, it is believed that the PRP-derived growth factors play the leading role. A study by Marini et al demonstrated that the expression of c-MYC gene was up-regulated in endometrial cells which were cultured with PRP, in comparison to the expression levels found in untreated cells (45). c-MYC is involved in cell in vivo 33: 515-521 (2019) proliferation and growth, and is activated by EGF, a component of PRP.…”

The Effects of Leukocyte- and Platelet-Rich Plasma (L-Prp) and Pure Platelet-Rich Plasma (P-Prp) an a Rat Endometriosis Model

“…There are also cytokines such as IL-1, IL-8 in PRP 50 . Marini et al showed that PRP reduced IL-1B and IL-8 release in endometrial tissues and they attributed the anti-inflammatory effect of PRP to this reduction 51 . Some of the cytokines held responsible for the pathophysiology, which are shown to increase in the peritoneal fluid in endometriosis, are IL-1 and IL-8 44 .…”

Platelet-rich plasma therapy and reproductive medicine

“…Considering that cell proliferation and tissue growth depend not only on single growth factors, but on an overall favorable environment, another possible explanation on the mode of action of PRP on endometrial growth and receptivity is via its anti-microbial, anti-inflammatory properties, as demonstrated on human chondrocytes [19] and on equine and bovine uterine infections [20][21][22][23]. In humans, chronic endometritis (CE) has a high prevalence among infertile patients (range 2.8 to 46%) [24,25].…”