Effects of plant growth-promoting rhizobacteria on nodulation of Phaseolus vulgaris L. are dependent on plant P nutrition

Remans, Roseline; Croonenborghs, Anja; Gutiérrez, Roldán Torres; Michiels, Jan; Vanderleyden, Jozef

doi:10.1007/s10658-007-9154-4

Cited by 57 publications

(31 citation statements)

References 55 publications

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“…Previous work using the co-inoculation of free-living plant growth-promoting bacteria (Azospirillum spp.) with rhizobia reported positive effects due to increased nodulation [7,16,31]. We did not observe any such effect of co-inoculation (rhizobia + Herbaspirillum) on nodulation (number or dry weight, Table 4) in both the independent experiments; therefore, the positive effects of the mixed inoculation on bean plants is not directly attributable to BNF.…”

Section: Discussionmentioning

confidence: 55%

“…These effects were attributed to a reduction in the ethylene production by the bacterial enzyme aminocyclopropane-1-carboxylate deaminase and the maintenance of a normal level of plant endogenous abscisic acid [14]. Some reports on mixed inoculations of rhizobia and Azospirillum on common beans showed enhanced levels of nodulation [15,16] and higher crop yields [7,17].…”

Section: Introductionmentioning

confidence: 99%

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Mixed rhizobia and Herbaspirillum seropedicae inoculations with humic acid-like substances improve water-stress recovery in common beans

Melo

Olivares

Médici

et al. 2017

Chem. Biol. Technol. Agric.

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Background: Common beans are a staple food in many developing countries. However, changes in global precipitation patterns, particularly short droughts during the rainy season, have affected crop production because nodulation is greatly affected by water shortages. Plant growth-promoting bacteria, together with humic substances, can alleviate the negative effects of soil stresses, including drought. Aims:This work aimed to evaluate the effects of co-inoculations of rhizobia and Herbaspirillum seropedicae in the presence of humic acid-like substances isolated from vermicompost on the recovery of common beans after a controlled water stress. Methods:Two independent experiments were conducted in a greenhouse using Phaseolus vulgaris cv. Grafite and cv. Bonus, which originated in Brazil and Mozambique, respectively. The soil humidity was maintained at field capacity in pots, and the water suppression was induced at the pre-flowering stage. After 12 days, the water was restored, and the beans were evaluated. Results:In the first experiment with 'Grafite' beans, the relative water content of the foliar disks was significantly higher in the co-inoculated treatment, as were the numbers and masses of nodules. The phenylalanine ammonia lyase activity was induced by drought, and its activity was higher in co-inoculated plant leaves. In the second experiment, the rate of net photosynthesis and stomatal conductance were also evaluated. The recuperation of common beans after water stress was improved by co-inoculation with rhizobia and H. seropedicae in the presence of humic acid-like substances.

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Mixed rhizobia and Herbaspirillum seropedicae inoculations with humic acid-like substances improve water-stress recovery in common beans

Melo

Olivares

Médici

et al. 2017

Chem. Biol. Technol. Agric.

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“…The inocula were prepared as reported by Remans et al (2007). Cells were washed twice with 10 mM MgSO 4 and resuspended in 10 mM MgSO 4 at a density of 10 7 colony forming units (CFU) ml -1…”

Section: Greenhouse Assaymentioning

confidence: 99%

Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

et al. 2017

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Rhizobium-legume symbioses play relevant roles in agriculture but have not been well studied in Ecuador. The aim of this study was to characterize the genetic and phenotypic diversity of Rhizobium isolates associated with Phaseolus vulgaris from southern Ecuador. Morphocultural characterization, biochemical tests and physiological analyses were conducted to authenticate and determine the diversity of bacteria Rhizobium-like isolates. The genetic diversity of the isolates was determined by molecular techniques, which consisted of bacteria DNA extraction and amplification and sequencing of the 16S rRNA gene. The nodulation parameters and nitrogen fixation for P. vulgaris under greenhouse conditions were also assessed to determine the phenotypic diversity among isolates. Furthermore, bacteria indole-acetic-acid production was evaluated by the colorimetric method. Morpho-cultural and biochemical characteristic assessments demonstrated that Rhizobium-like bacteria was associated with the P. vulgaris nodules. The diversity among the isolates, as determined by physiological analyses, revealed the potential of several isolates to grow at different pH values, salinity conditions and temperatures. Partial sequencing of the 16S rRNA gene identified the Rhizobium genus in every sampling site. From a total of 20 aligned sequences, nine species of Rhizobium were identified. Nodule formation and biomass, as well as nitrogen fixation, showed an increase in plant phenotypic parameters, which could be influenced by IAA production, especially for the strains R. mesoamericanum NAM1 and R. leguminosarum bv. viciae COL6. These results demonstrated the efficiency of native symbiotic diazotrophic strains inoculants for legume production. This work can serve as the basis for additional studies of native Rhizobium strains and to help spread the use of biofertilizers in Ecuadorian fields.Index terms: Phaseolus vulgaris; diazotrophic bacteria; 16S rRNA gene; nodulation; indole acetic acid. RESUMOA simbiose Rhizobium-leguminosa desempenha um relevante papel na agricultura, entretanto não tem recebido suficiente atenção de estudos científicos no Equador. O objetivo deste artigo foi caracterizar a diversidade genética e fenotípica de isolados de Rhizobium associados com Phaseolus vulgaris do sul do Equador. A caracterização morfo-cultural, testes bioquímicos e análises fisiológicas foram realizados para autenticar e determinar a diversidade de isolados de bactérias Rhizobium. A diversidade genética foi determinada por por técnicas moleculares consistindo na extração de DNA genômico bacteriano, amplificação e sequenciamento parcial do gene 16SrRNA; e parâmetros de nodulação e fixação de nitrogênio de P. vulgaris sobre condições de estufa foram testados para determinar a diversidade fenotípica entre os isolados. Além disso, a produção de ácido indolacético foi avaliada por um método colorimétrico. A análise fisiológica da diversidade entre os isolados revelou o potencial de crescimento de diversos isolados em diferentes níveis de pH, sa...

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“…The first step of these bacteria in growth promotion is by colonizing the root. PGPR have a vital role in the host nodulation response [92].…”

Section: Co-inoculation Of Plant With Pgprmentioning

confidence: 99%

Plant Growth Promoting Rhizobacteria: Mechanism and Current Prospective

Kundan¹,

Pant²

2015

J Fertil Pestic

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Despite the phytotoxicity of olive-mill solid waste (OMSW) due to its high polyphenols content, OMSW have fertilizer characteristics, which make it a potential source for organic fertilization. Composting of OMSW treatment process was conducted in this study to eliminate the phytotoxicity and solve the environmental impact of this waste. Recycling of OMSW was carried out via composting of six batches of trials using equal proportions of OMSW, cow manure (C) and wheat straw (W). The treatment process was performed at two time intervals (two and five months), after each one, the recipient species (Vicia faba L.) was planted. The results showed the efficiency of composting in reducing OMSW original toxicity after two months than five months. The germination percentage and the plumule and radicle lengths of V. faba showed a significant improvement when the OMSW was composted with C at different proportions before using as soil amendments. Besides, the total biomass was noticeably increased at the high concentration of C-OMSW. Similarly, the total pigments concentration in V. faba was increased by using various composts after two months, where the highest pigment content was observed at 40% W-OMSW treatment.The maximum uptake of potassium and sodium was recorded through the application of W-OMSW compost to soil after two months. Furthermore, the C-OMSW composts showed the highest concentration of nitrogen, calcium, iron and manganese. However, the C-W-OMSW composts recorded the highest concentration of phosphorous, magnesium and copper. Finally, this study developed a low cost treatment that will enable the growers to convert OMSW into a natural nontoxic compost rich with essential nutrients which have positive effects on plants growth.

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Effects of plant growth-promoting rhizobacteria on nodulation of Phaseolus vulgaris L. are dependent on plant P nutrition

Cited by 57 publications

References 55 publications

Mixed rhizobia and Herbaspirillum seropedicae inoculations with humic acid-like substances improve water-stress recovery in common beans

Mixed rhizobia and Herbaspirillum seropedicae inoculations with humic acid-like substances improve water-stress recovery in common beans

Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

Plant Growth Promoting Rhizobacteria: Mechanism and Current Prospective

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