Effects of plant extract antioxidative phenolic compounds on energetic status and viability of Saccharomyces cerevisiae cells undergoing oxidative stress

Chanaj-Kaczmarek, Justyna; Wysocki, Michał; Karachitos, Andonis; Wojcińska, Małgorzata; Bartosz, Grzegorz; Matławska, Irena; Kmita, Hanna

doi:10.1016/j.jff.2015.04.046

Cited by 14 publications

(9 citation statements)

References 65 publications

(71 reference statements)

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“…Additionally, the antioxidant activity was tested using a method of the Saccharomyces cerevisiae yeast strain without gene sod1. The decoction and 70% extract improved the viability of S. cerevisiae due to antioxidative activity of phenolic compounds especially phenolic acids [ 32 ]. The antioxidant properties of GP proven in our analysis as well as in the other research studies suggest that this plant species may exert a beneficial effect in the symptoms associated with inflammation.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav.

et al. 2018

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The objective of this study was to evaluate the usefulness of a hydroalcoholic extract from Galinsoga parviflora herb (GP) in some aspects of the endothelial cell function necessary for anti-inflammatory activity and wound healing and relate these to the GP phytochemical profile. This study demonstrated that the GP extract caused a dose-dependent reduction of IL-6 secretion on IL-1β-stimulated endothelial cells. The IL-6 release was decreased to 33% ± 9% while this did not influence the IL-6 secretion without stimulation. Additionally, the GP extract exhibited an anti-hyaluronidase activity (IC50 = 0.47 mg/mL), which was evidently stronger than the positive control kaempferol (IC50 = 0.78 mg/mL) as well as a moderate and concentration-dependent, antioxidant activity. The results of the scratch assay showed that exposure of the endothelial cells to GP induced complete healing of the damage after 12 h of the study. The phytochemical profile of the extract was studied by using spectrophotometric (total amount of polyphenols and flavonoids) and UPLC (phenolic acids) methods. The main compound in the GP extract was a chlorogenic acid (2.00 ± 0.01 mg/g by UPLC). The total content of polyphenols was 98.30 ± 0.14 mg of chlorogenic acid equivalent/g of the dry herb and content of flavonoids amounted to 6.15 ± 0.41 mg quercetin equivalent/g of the dry herb. Moreover, the presence of flavonoids in G. parviflora was provided after their isolation and identification by spectroscopic methods. In conclusion, it demonstrated that application of GP in the treatment of skin lesions gives possibility of wound healing based on antioxidant, anti-inflammatory, and hyaluronidase-inhibiting activities of G. parviflora herb extract.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav.

et al. 2018

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“…In this method, the reagent thiobarbituric acid generates adduct with malondialdehyde, which is detectable spectrophotometrically at 532 nm. Besides the aforementioned method, cell viability assays are also employed in assessing oxidative damage in yeast, which evaluates the stress tolerance increase caused by treatment with antioxidant compounds [71]; mitochondrial function assays, since many apoptotic processes start in this organelle [72]; measurement of intracellular reactive oxygen species formation, using 2,7-dichloroluorescein as indicator [71,73]; protein carbonylation tests [74,75], which is also formed as consequence of oxidative damage; assessment of energetic metabolism and enzymatic activity associated with the stress response [67,74], among other methods.…”

Section: Antioxidant Activity Assaysmentioning

confidence: 99%

Honey as a Functional Food

Luchese¹,

Prudêncio²,

Guerra³

2017

Honey Analysis

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The most well-known functional properties of honey are its antioxidant and antimicrobial activities. The bioactive components of honey are afected by the lora from which it is produced and by geographical variations. Phenolic compounds promote, among other activities, high antioxidant action, being capable of minimizing intracellular oxidative damage associated with cellular aging, apoptosis and neurodegenerative diseases. A living cell system would provide a beter platform for determining antioxidant activity, since the bioactive honey compounds can act modulating antioxidant defense gene expression. Indeed, phenolic compounds, amino acids and reducing sugars are among the substances responsible for honey antioxidant activity. Most of phenolic compounds also exert antimicrobial activity against a number of pathogens and spoilage microorganisms. The antimicrobial activity of honey is also due to the action of enzymes. In addition, honey was found to contain lactic acid bacteria (LAB), which itself produce a myriad of active compounds that remain in variable amounts in mature honey. In addition, these antioxidant compounds might play a key role as prebiotic, protecting and stimulating growth of probiotic bacteria. Oligosaccharides present in honey are well-known prebiotic substances stimulating growth, activity and protecting probiotic bacteria during passage through the gastrointestinal tract and during storage of the products. This chapter describes the main bioactive components of honey, especially with respect to the phenolic compounds and their antioxidant activity and assay methods.

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“…G. parviflora Cav., popularly known as gallant soldier or picão branco, is an herbaceous native from South America, abundant in agricultural areas of temperate and subtropical regions of the world [8], [9]. This plant is considered a non-conventional food plant and its leaves are used in salads, spices and sauteed in countries of North and South America and South Africa [8], [10], [11], [12], [13].…”

Section: Introductionmentioning

confidence: 99%

“…Due to its high vitamin C concentration, this herb is also used to treat colds, flu, cold sores and to prevent scurvy [8], [14], [15], [16]. Phenolic compounds constitute the main class of phytochemicals isolated from this species, however, flavonoids, aromatic esters, diterpenoids, caffeic acid, steroids and phenolic acid derivatives were also isolated in G. parviflora [8], [9], [11], [14], [17].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening, Antioxidant, Anti-Cytotoxic and Anticancer Effects of Galinsoga Parviflora and Vernonia Polyanthes (Asteraceae) Extracts

Feleti

Aleluia

Gervásio

et al. 2020

Int. J. Res. Granthaalayah

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The study was designed to investigate the chemical composition and the biological effects of G. parviflora and V. polyanthes ethanolic extracts in vitro. Total content of phenols, flavonoids and tannins was quantified by spectrophotometry; chemical characterization was permed by mass spectrometry (ESI (-) FT-ICR MS and APCI (+) FT-ICR MS analysis). Antioxidant activities were determined by FRAP and Fe2+ chelating methods. Extracts cytotoxicity was evaluated in human lymphocytes, sarcoma-180 (S-180) and human gastric adenocarcinoma (AGS) cells, by MTT assay. V. polyanthes presented higher total content of tannins and G. parviflora presented higher amount of phenols and flavonoids. Chemical characterization showed the presence of flavonoids, phenolic acids and sesquiterpene lactones in V. polyanthes extract, and steroids, phenolic acids and fatty acids (Poly Unsaturated Fatty Acids - PUFA) in G. parviflora extract. V. polyanthes extract stood out in the Fe2+ chelation test. G. parviflora extract did not present outstanding antioxidant results in the tested protocols. Both species showed a tendency to promote cytotoxicity in human lymphocyte cells. Regarding the antiproliferative effect, both species were able to reduce S-180 cell viability and G. parviflora extract showed high antiproliferative potential in the assay with AGS cells. These findings reinforce the medicinal use of these plants, as well as suggest their potential use for the development of new drugs and for the treatment of cancers.

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Effects of plant extract antioxidative phenolic compounds on energetic status and viability of Saccharomyces cerevisiae cells undergoing oxidative stress

Cited by 14 publications

References 65 publications

Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav.

Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav.

Honey as a Functional Food

Phytochemical Screening, Antioxidant, Anti-Cytotoxic and Anticancer Effects of Galinsoga Parviflora and Vernonia Polyanthes (Asteraceae) Extracts

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