Effects of phytoestrogenic isoflavones from red clover (<i>Trifolium pratense</i> L.) on experimental osteoporosis

Occhiuto, Francesco; Pasquale, R. De; Guglielmo, Giordano de; Palumbo, Dora Rita; Zangla, Giuseppe; Samperi, Stefania; Renzo, A. Di; Circosta, Clara

doi:10.1002/ptr.2037

Cited by 63 publications

(44 citation statements)

References 24 publications

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“…In the present study, PCS effectively inhibited or refined climacterium symptoms, including obesity, hyperlipidemia, hepatic (47)(48)(49)(50). Previous studies have explored alternative therapies, such as the use of phytoestrogens, to relieve menopausal symptoms.…”

Section: Discussionmentioning

confidence: 62%

Anti-climacterium effects of pomegranate concentrated solutions in ovariectomized ddY mice

Kang¹,

Choi²,

Kim³

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. In the present study, the complex anti-climacterium potential of standardized pomegranate concentrated solution (PCS) was investigated using bilateral ovariectomy (OVX) female ddY mice. Changes in body weight and gain during experimental periods, food consumption, serum estradiol levels, total body and abdominal fat densities, abdominal fat pads, and uterus weights were observed, along with the histopathology of abdominal fat pads and uterus for anti-obesity and estrogenic effects. In addition, liver weights, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, and histopathological inspections were performed to explore the hepato-protective effects. Serum total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein, and triglyceride (TG) levels were monitored for hypolipidemic effects with total body and femur mean bone mineral density (BMD), right femur wet, dry and ash weights, strength, serum osteocalcin, bone-specific alkaline phosphatase (bALP) contents, and histological and histomorphometrical analyses for anti-osteoporosis activity. As a result of OVX, notable increases in body weight and gains, food consumption, abdominal fat mass densities, weights of abdominal fat pads deposited in the abdominal cavity, and serum AST, ALT, TC, LDL, TG, and osteocalcin levels were observed, along with decreases in the uterus, liver, and femur weights, mean total body and femur BMD, femur strength, serum bALP, and estradiol levels. In addition, marked hypertrophic alterations in adipocytes located in the deposited abdominal fat pads, liver steatosis, uterine disused atrophic changes, and decreases in bone mass and structures of the femur were also observed in OVX control mice with significant increases in bone resorption markers based on histopathological and histomorphometrical analysis. However, these estrogen-deficient climacterium symptoms were significantly (P<0.05 or P<0.01) inhibited after 84 days of continuous treatment with estradiol and PCS (1, 2 and 4 ml/kg), respectively. The present results suggested that PCS was able to effectively inhibit or refine the climacterium symptoms, including obesity, hyperlipidemia, hepatic steatosis, and osteoporosis, induced by OVX in ddY mice.

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Section: Discussionmentioning

confidence: 62%

Anti-climacterium effects of pomegranate concentrated solutions in ovariectomized ddY mice

Kang¹,

Choi²,

Kim³

et al. 2017

Experimental and Therapeutic Medicine

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“…Isoflavones are used for their health beneficial roles such as protection against hormone dependent osteoporosis and cancers in human and animal systems 43,44 . There have been efforts to engineer their biosynthesis in nonleguminous plants, but very limited success could be achieved [45][46][47][48][49][50][51] .…”

Section: Discussionmentioning

confidence: 99%

Untitled

2017

IJPSR

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The present study was initiated to quantify the Isoflavones content that accumulated in soybean transgenics with RnPPARγ gene by using high performance liquid chromatography. The effects of methanolic leaves extract of transformed soybean in 3T3-L 1 adipocyte cell line. The RnPPARγ transgenic soybean variety JS335 was developed and transgenics were confirmed by PCR and Southern blot techniques. The quantification of isoflavones compounds by HPLC showed that methanolic leaves extracts of transformed soybean contained Genistein (1.67µg/g extract), Daidzein (2.76µg/g extract), Rutin (32.06µg/g extract) and Quercetin (48.40µg/g extract) and methanolic leaves extracts of nontransformed soybean contained Genistein (1.23µg/g extract), Daidzein (2.33µg/g extract), Rutin (26.16µg/g extract) and Quercetin (43.03µg/g extract) by comparing the chromatogram of the reference standards. In the present study revealed that secondary metabolite content was higher in the transformed plant than non-transformed plants. After treatment of transformed methanolic leaves, extracts on 3T3-L 1 mature adipocytes significantly ameliorated lipid droplet accumulation and droplet reduction in adipocytes might be the result of lipid metabolism by lipolysis.

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“…Literatura przedmiotu zawiera dowody na skuteczność osteoprotekcyjną izoflawonoidów z czerwonej koniczyny [27,28]. Atkinson i wsp.…”

Section: Promensilunclassified

Stanowisko Zespołu Ekspertów Polskiego Towarzystwa Menopauzy i Andropauzy dotyczące zastosowania preparatów PROMENSIL® i PROMENSIL FORTE® u kobiet w okresie około- i pomenopauzalnym

Dębski¹,

Paszkowski²,

Pertyński³

et al. 2013

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Effects of phytoestrogenic isoflavones from red clover (Trifolium pratense L.) on experimental osteoporosis

Cited by 63 publications

References 24 publications

Anti-climacterium effects of pomegranate concentrated solutions in ovariectomized ddY mice

Anti-climacterium effects of pomegranate concentrated solutions in ovariectomized ddY mice

Untitled

Stanowisko Zespołu Ekspertów Polskiego Towarzystwa Menopauzy i Andropauzy dotyczące zastosowania preparatów PROMENSIL® i PROMENSIL FORTE® u kobiet w okresie około- i pomenopauzalnym

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