Abstract:The present study was initiated to quantify the Isoflavones content that accumulated in soybean transgenics with RnPPARγ gene by using high performance liquid chromatography. The effects of methanolic leaves extract of transformed soybean in 3T3-L 1 adipocyte cell line. The RnPPARγ transgenic soybean variety JS335 was developed and transgenics were confirmed by PCR and Southern blot techniques. The quantification of isoflavones compounds by HPLC showed that methanolic leaves extracts of transformed soybean con… Show more
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