Effects of phosphate-solubilizing bacteria, native microorganisms, and rock dust on Jatropha curcas L. growth

Abstract

Microorganisms with the ability to release nutrients to the soil from insoluble sources may be useful for plant cultivation. We evaluated the growth-promoting effect on Jatropha curcas L. of phosphate-solubilizing bacteria (PSB) and the native microbiota in soil with or without rock dust. J. curcas L. is important for biodiesel production. The experiments were performed in a greenhouse under a random-statistical design with 14 replicates. The soil received increasing dosages of rock dust. The presence of resident microorganisms and PSB inoculum was correlated with plant height, biomass production, and phosphorus content in plants for 120 days. Native soil microorganisms were detected and identified using denaturing gradient gel electrophoresis and DNA sequence analysis. Several bacterial populations belonged to the genus Bacillus. Populations associated with the phyla Chytridiomycota and Ascomycota were detected among the fungi. The best results for the variable plant height were correlated with the presence of resident microbiota and rock dust until the end of the experiment. The largest biomass production and the highest content of phosphorus occurred in the presence of soil-resident microbiota only up to 120 days. No significant effects were observed for biomass production with the use of PSB combined with rock dust. J. curcas L. under the influence of only resident microbiota showed the best plant growth results. Future research will focus on the specificity of resident microbiota activity in plant growth promotion and the isolation of these microorganisms to produce a new inoculum to be tested in various plants.

Phosphate Solubilizing Microorganisms: Promising Approach as Biofertilizers

“…Phosphorus (P) is one of the major growth-limiting macronutrients required for proper plant growth, particularly in tropical areas, due to its low availability in the soil [1]. It accounts for between 0.2 and 0.8% of the dry weight of plants [2], and it is contained within nucleic acids, enzymes, coenzymes, nucleotides, and phospholipids.…”

Research Article Phosphate solubilizing activity of native soil microorganisms from the rhizosphere of <i>Jatropha</i> <i>curcas</i> and from phosphate-solubilizing bacteria inoculum

“…DNA extraction, DGGE and Sequence Analysis to determine the profile of the soil microbial community were carried out according to Santana et al, (2017). Species of Bacillus from rhizosphere were identified: B. subtilis, B. amyloliquefaciens, B. atropheus and B. vallismortis.…”

“…These microorganisms can supply the plant with phosphorus through the enzymatic activity and production of organic acids (Mohanty et al, 2013). Phosphate-solubilizing microorganisms associated with a low-solubility phosphorous source, such as in natural rocks, has been successful in various crops, resulting in improvements in growth and production (Rodríguez and Fraga, 1999;Panhwaret al, 2011;Jha et al, 2012;Santana et al, 2017).…”

“…We extended from the work of Santana et al (2017), who examined the effects of bacteria and other microorganisms on J. curcasgrowth. Here, we evaluated the phosphorus content in leaves of Jatropha curcas inoculated with native microbiota and phosphate solubilizing bacteria in rhizospheric soil with or without rock dust and enzymatic microbial activity.…”

Studies on the acid-production characteristics of Bacillus megaterium strain P17

“…The activation of organic acids on phosphorus in soil exists obviously and the activating ratio increases with uniform growth trend (1). The organic acids affect the activation of invalid and sustained release phosphorus largely.…”