Background and ObjectivesThis study aimed to compare the quality of purple whole wheat flours (WWFs), which exhibit various health benefits. An ultracentrifugal mill was used to prepare purple WWF samples from four distinct purple wheat cultivars (Ariheukchal, AC; Ariheuk, AH; Arijinheuk, AJ; and Sintong, ST) each with different kernel hardness values and amylose contents.FindingsThe average particle diameter (d50) of the WWFs (ST < AJ < AH < AC) was positively correlated with the kernel hardness. The amylose contents of the WWFs increased in the order AC < AH < AJ < ST, whereas their solvent retention capacities (SRCs) in water and sodium carbonate solutions followed the reverse order, similar to the d50. SDS sedimentation volumes did not correlate with the protein contents. ST showed significantly lower total phenolic and anthocyanin contents and antioxidant activities than the other varieties.ConclusionsKernel hardness and amylose content significantly influenced the particle size, damaged starch content, SRC, and pasting properties of the WWFs. Additionally, the nutritional components and antioxidant activities of AC, AH, and AJ suggested strong potential for diverse functional products.Significance and NoveltyPurple WWFs with different kernel hardness values and amylose contents were compared scientifically for the first time.