Effects of pH, Potential, and Deposition Time on the Durability of Collagen Electrodeposited to Titanium

Abstract

Collagen is expected to work as a bonding agent of soft and hard tissues to solid materials. In this study, the electrodeposition of collagen to a titanium (Ti) surface under various conditions, i.e., the pH of the collagen solution, potential, and electrodeposition time, was performed to understand the optimal electrodeposition conditions for the immobilization of collagen to Ti. The effects of these conditions on the thickness and residual ratio of the collagen layer after shaking in water were evaluated by ellipsometry, scanning probe microscopy, and X-ray photoelectron spectroscopy. Collagen molecules were attracted to Ti cathode and immobilized with high durability by combining electrodeposition conditions, pH 5, alternating potential between À1 V and +1 V vs. SCE with 1 Hz, and 1800 s. The surface of this electrodeposited collagen layer was smooth and uniform maintaining the collagen fibril and natural structure. On the other hand, the collagen layer immobilized by immersion technique in a collagen solution, was rough and irregular. Electrodeposition with alternating potential at pH 5 for 1800 s is a much more appropriate technique to immobilize collagen to Ti than the conventional immersion technique.

Metals and Medicine

“…49) 5.3 Soft tissue adhesion, antibacterial property, and antithrombotic property Type I collagen has been strongly electrodeposited, with a mesh form, on a Ti surface using a sine wave with a positive and negative potential to improve soft tissue. 50) The electrodeposition of PEG diamine (NH 2 PEGNH 2 ), modified with amine groups on both terminals, results in a U-shaped immobilization (as shown in Fig. 6).…”

A comprehensive review of techniques for biofunctionalization of titanium

“…Type I collagen is grafted through glutaraldehyde as a crosslinking agent [ 60 ]. For the electrodeposition, it is found that an alternating current between -1 V and + 1V vs. saturated calomel electrode with 1 Hz is effective to immobilize type I collagen to Ti and durability in water is high [ 61 ]. The immobilized collagen fiber network image is shown in Fig.…”

Research and development of metals for medical devices based on clinical needs

“…Type I collagen is grafted through glutaraldehyde as a crosslinking agent [112]. For electrodeposition, the current alternating at 1 Hz between −1 V and +1 V versus SCE is effective to immobilize type I collagen on Ti, and the durability in water is high [113].…”