Effects of pH control and concentration on microbial oil production from Chlorella vulgaris cultivated in the effluent of a low-cost organic waste fermentation system producing volatile fatty acids

Cho, Hyun Uk; Kim, Young Beom; Choi, Yun‐Nam; Xu, Xu; Shin, Dong Yun; Park, Jong

doi:10.1016/j.biortech.2014.09.069

Cited by 68 publications

(16 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the carbon taken from the substrate and incorporated into the biomass, was 55 ± 4%. A similar carbon yield of 52% was also reported with a mixotrophic culture of C. vulgaris on raw acidogenic fermentation effluent [9]. Interestingly, this yield is significantly higher than previous values reported on synthetic DF effluents, 42% and 44%, for C. sorokiniana and C. protothecoides, respectively [3,10].…”

Section: Microalgae Growth On Sterilized and Unsterilized Dark Fermensupporting

confidence: 82%

See 1 more Smart Citation

Raw dark fermentation effluent to support heterotrophic microalgae growth: microalgae successfully outcompete bacteria for acetate

et al. 2015

View full text Add to dashboard Cite

Section: Microalgae Growth On Sterilized and Unsterilized Dark Fermensupporting

confidence: 82%

“…DF effluents also contain substantial amounts of nitrogen and phosphorus that are required to sustain the heterotrophic growth of microalgae. Cho et al pointed out that DF effluent can be regarded as a good medium for growing heterotrophic microalgae in a biorefinery [9].…”

Section: Introductionmentioning

confidence: 99%

Raw dark fermentation effluent to support heterotrophic microalgae growth: microalgae successfully outcompete bacteria for acetate

et al. 2015

View full text Add to dashboard Cite

“…Furthermore, microalgae showed extremely high lipid accumulation ability, reaching up to 90% (wt/wt) of the dry weight of lipids under certain conditions (Spolaore, Joannis‐Cassan, Duran, & Isambert, ). To obtain an economically viable lipid production from microalgae, current research efforts have been conducted to favor microalgal growth on digestates proceeding from AD of wastewater, food waste or manure as a low‐cost carbon source (Cho et al, ; Fei, Fu, Shang, Brigham, & Chang, ; Shin et al, ). However, the high ammonium concentration in AD effluents might be inhibitory for microalgae growth (Gonzaleá‐Fernández & Muñoz, ).…”

Section: Oleaginous Microorganisms For Oil Productionmentioning

confidence: 99%

Volatile fatty acids as novel building blocks for oil‐based chemistry via oleaginous yeast fermentation

Llamas

Magdalena

González‐Fernández

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

Microbial oils are proposed as a suitable alternative to petroleum‐based chemistry in terms of environmental preservation. These oils have traditionally been studied using sugar‐based feedstock, which implies high costs, substrate limitation, and high contamination risks. In this sense, low‐cost carbon sources such as volatile fatty acids (VFAs) are envisaged as promising building blocks for lipid biosynthesis to produce oil‐based bioproducts. VFAs can be generated from a wide variety of organic wastes through anaerobic digestion and further converted into lipids by oleaginous yeasts (OYs) in a fermentation process. These microorganisms can accumulate in the form of lipid bodies, lipids of up to 60% wt/wt of their biomass. In this context, OY is a promising biotechnological tool for biofuel and bioproduct generation using low‐cost VFA media as substrates. This review covers recent advances in microbial oil production from VFAs. Production of VFAs via anaerobic digestion processes and the involved metabolic pathways are reviewed. The main challenges as well as recent approaches for lipid overproduction are also discussed.

show abstract

“…Environmental conditions including nutrient composition and physiological state of the cells affect the biomass and the degree of saturation of the lipids (Cho et al 2015). At this juncture, the present study aims at optimizing the algal media to enhance the biomass and lipid production of C. oleofaciens.…”

Section: Graphical Abstractmentioning

confidence: 99%

Novel Media for Lipid Production of Chlorococcum oleofaciens : A RSM Approach

Pauline¹,

Achary²

2019

View full text Add to dashboard Cite

The algal medium was optimized to increase the biomass and lipid production of Chlorococcum oleofaciens. The significant variables were screened and chosen from previously reported algal culture media using Plackett Burman Design (PBD). Optimization of the significant variables were performed using central composite design. The Pareto chart for PBD revealed that the salts such as sodium bicarbonate, sodium nitrate, potassium nitrate and ferrous sulphate had enhanced the biomass and lipid production. The variables and its effect on the responses were further studied by central composite design (CCD). A new medium was formulated based on the response surface methodology. The predicted concentration of NaHCO 3 , NaNO 3 , KNO 3 , MgSO 4 .7H 2 O were found to be 6.75 g/L, 0.75 g/L, 1.88 g/L and 0.35 g/L respectively. The actual and the predicted total lipid yield for the optimized media was around 0.74 g/L and 0.78 g/L respectively. The optimal medium has been named as AM medium. Growth and the lipid yields of C. oleofaciens were found higher in AM medium. The specific growth rates of C. oleofaciens in AM and CFTRI media were found to be 0.14 day-1 and 0.19 day-1 respectively. The biomass produced by the optimized AM medium was found to be 2.7 times greater compared to the CFTRI medium. The lipid was extracted and GC-MS was performed which revealed that the fatty acids were predominantly of the class C15:0, C18:0, C16:0 and C12:0. It is concluded that besides lipid content, AM medium increased the cell number leading to the increase in biomass.

show abstract

Effects of pH control and concentration on microbial oil production from Chlorella vulgaris cultivated in the effluent of a low-cost organic waste fermentation system producing volatile fatty acids

Cited by 68 publications

References 31 publications

Raw dark fermentation effluent to support heterotrophic microalgae growth: microalgae successfully outcompete bacteria for acetate

Raw dark fermentation effluent to support heterotrophic microalgae growth: microalgae successfully outcompete bacteria for acetate

Volatile fatty acids as novel building blocks for oil‐based chemistry via oleaginous yeast fermentation

Novel Media for Lipid Production of Chlorococcum oleofaciens : A RSM Approach

Contact Info

Product

Resources

About