Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model

Loh, Foo-Hoe; Bongso, Ariff; Fong, Chui‐Yee; Koh, Dow-Rhoon; Lee, Szu‐Hee; Zhao, Hui-Qin

doi:10.1016/s0015-0282(99)00292-7

Cited by 28 publications

(16 citation statements)

References 25 publications

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“…Although Loh et al [17] have suggested that activated peritoneal macrophages and derived soluble factors could also stimulate cellular endometrial proliferation in vitro, it is likely that the activation of physiological defences with rIL-2 in vivo and in situ (intracyst) is different. Some authors have used intraperitoneal infusions of rIL-2 in malignant ascites in patients with gastrointestinal and ovarian cancers [18], or intravesical continuous perfusion in patients with superficial bladder cancer in different phase I studies, which proved to be safe and yielded good clinical results [19].…”

Section: Discussionmentioning

confidence: 99%

GnRH Analogues, Transvaginal Ultrasound-Guided Drainage and Intracystic Injection of Recombinant Interleukin-2 in the Treatment of Endometriosis

Acién

Quereda

Gómez‐Torres

et al. 2003

Gynecol Obstet Invest

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We performed a double-blind, randomised controlled trial to evaluate the results of ultrasound-guided aspiration of endometriomas under the effect of GnRH analogues and a possible additional beneficial effect by leaving 600,000 IU of recombinant interleukin-2 (rIL-2) in the cysts. Twenty-four women with endometriosis-related symptoms, increased values of CA-125 and transvaginal ultrasonography showing endometriomas >3 cm who were initially sent to us for laparotomy and conservative surgery for endometriosis were included. Main outcome measures were severity of symptoms, size and percentage of echographical reduction of endometriomas and CA-125 levels after 2 menses post-GnRH analogues. Secondary outcome measures were the time until recurrence of abnormal parameters and the need for surgery after treatment. We found moderate clinical results after treatment with drainage plus GnRH analogues and significantly improved results in women having received rIL-2 intracystically. There were no side effects. Two out of 3 previously infertile patients became pregnant after therapy. Though the rates of recurrence of endometriomas ≧3 cm were similar in both groups, the period until recurrence was significantly greater when rIL-2 was used, and the rates of recurrence of symptoms and increased CA-125 values were also significantly lower in patients who received rIL-2. Surgery was finally performed on 10 patients (4 with and 6 without previous rIL-2 treatment) during follow-up (30 ± 12.7 months). These findings led to the conclusion that transvaginal ultrasound-guided puncture and aspiration of endometriomas under endometrial suppressive therapy with GnRH analogues have some value for endometriosis treatment, improving the clinical manifestations and avoiding some surgical therapies, and that rIL-2 left in the cyst increases these beneficial effects significantly.

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Section: Discussionmentioning

confidence: 99%

GnRH Analogues, Transvaginal Ultrasound-Guided Drainage and Intracystic Injection of Recombinant Interleukin-2 in the Treatment of Endometriosis

Acién

Quereda

Gómez‐Torres

et al. 2003

Gynecol Obstet Invest

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“…4 A role for inflammatory factors and the immune system has long been posited in the development of endometriosis. [3][4][5][6][7][8][9] The numbers of white blood cells in the peritoneal fluid of women with endometriosis is greater than that in women without endometriosis, 10 and there are greater numbers of white blood cells in ectopic endometriotic tissue than eutopic endometrium. 11 Moreover, many of the genes that were shown to be differentially expressed in ectopic versus eutopic endometrium were associated with defense and immune functions, 12 thereby implicating an interaction between endometrial cells and cells of the immune system.…”

Section: Introductionmentioning

confidence: 99%

Two-Way Communication Between Endometrial Stromal Cells and Monocytes

et al. 2010

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Immune system cells and cells of the endometrium have long been proposed to interact in both physiological and pathological processes. The current study was undertaken to examine communication between cultured monocytes and endometrial stromal cells and also to assess responses of endometrial stromal cells for treatment with estradiol (E) in the absence and presence of medroxyprogesterone acetate (P). A telomerase-immortalized human endometrial stromal cell (T-HESC) line and the U937 monocyte cell line were used. Telomerase-immortalized human endometrial stromal cells were treated with E +/- P +/- monocyte conditioned medium; U937 were treated +/- T-HESC conditioned medium. Gene expression in response to treatment was examined by DNA microarray. Bidirectional communication, as demonstrated by changes in gene expression, clearly occurred between U937 monocytes and T-HESC.

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“…On the basis of the incidence discrepancy between menstrual reflux and prevalence of endometriosis, the mechanisms for proliferation, neoangiogenesis, and hormonal and immunologic responsiveness need to be analyzed (2,3). In endometriosis, resistance to apoptosis of endometrial cells (4) and their increased sensitivity to proliferation (5) suggest the possible pathogenesis of the disease.…”

mentioning

confidence: 99%

Endometrium from women with endometriosis shows increased proliferation activity

Park

Lee

Kim

et al. 2009

Fertility and Sterility

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Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model

Cited by 28 publications

References 25 publications

GnRH Analogues, Transvaginal Ultrasound-Guided Drainage and Intracystic Injection of Recombinant Interleukin-2 in the Treatment of Endometriosis

GnRH Analogues, Transvaginal Ultrasound-Guided Drainage and Intracystic Injection of Recombinant Interleukin-2 in the Treatment of Endometriosis

Two-Way Communication Between Endometrial Stromal Cells and Monocytes

Endometrium from women with endometriosis shows increased proliferation activity

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