Effects of pentoxifylline during<i>Schistosoma mansoni</i>infection in Swiss mice: an analysis of worm burden, fecundity and liver histopathology

Mati, Vitor Luís Tenório; Freitas, Ricardo Miguel Costa de; Melo, Alan Lane de

doi:10.1017/s0022149x09990733

Cited by 11 publications

(18 citation statements)

References 40 publications

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“…These observations were also reported by Utzinger et al [61], Suleiman et al [57] and Mati et al [39]. They found, the reduction in the worm recovery and egg density in treated mice was considered as a strong indication of the effectiveness of anti-Schistosoma drugs.…”

Section: Discussionsupporting

confidence: 75%

Schistosoma mansoni: Antiparasitic effects of orally administeredNigella sativaoil and/orChroococcus turgidusextract

Ali

Eldahab

Mansour

et al. 2016

Acta Biologica Hungarica

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Schistosoma mansoni is one of the parasites causing schistosomiasis, a disease which threatens millions of people all over the world. Traditional chemical drugs are not fully effective against schistosomaisis due to the evolving drug resistant worm strains, so exploring new remedies derived from natural products is a good way to fight schistosomiasis. In the present investigation two natural products, Nigella sativa oil and Chroococcus turgidus extract were used separately or in a combination to explore their effect on S. mansoni. The infected mice treated with Chroococcus turgidus extract or/and sativa seed oil showed a significant decrease in the total worm burden. The total number of deposited eggs by females of S. mansoni was significantly decreased in the liver of mice treated with Chroococcus turgidus extract or/and sativa seed oil. However, in the intestine, the number of eggs was significantly reduced in mice treated with algal extract and those treated with both algal extract and oil. Fecundity of female S. mansoni showed a significant decrease from mice treated with algal extract or/and sativa seed oil. According to SEM investigations the tegmental surface, oral and ventral suckers of worms also showed considerable changes; as the tubercles lost their spines, some are swollen and torn out. The suckers become edematous and enlarged while the tegmental surface is damaged due to the treatment with Chroococcus turgidus extract or/and sativa seed oil. In conclusion, the Nigella sativa oil and Chroococcus turgidus extract are promising natural compounds that can be used in fighting schistosomiasis.

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Section: Discussionsupporting

confidence: 75%

Schistosoma mansoni: Antiparasitic effects of orally administeredNigella sativaoil and/orChroococcus turgidusextract

Ali

Eldahab

Mansour

et al. 2016

Acta Biologica Hungarica

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“…The reduction in the worm recovery and egg density in treated mice was considered by several authors as a strong evidence of the efficiency of antischistosomal drugs (Utzinger et al 2002;Suleiman et al 2004;Mati et al 2010). The death of the worms due to treatment with antischistosomal drugs was attributed to metabolic disorders, mechanical destruction and muscular contraction of the treated worms (Andrews 1985;Harder et al 1987).…”

Section: Discussionmentioning

confidence: 97%

Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice

2011

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The repeated chemotherapy of schistosomiasis has resulted in the emergence of drug-resistant schistosome strains. The development of such resistance has drawn the attention of many authors to alternative drugs. Many medicinal plants were studied to investigate their antischistosomal potency. The present work aimed to evaluate antischistosomal activity of crude aqueous extract of ginger against Schistosoma mansoni. Sixteen mice of C57 strain were exposed to 100 ± 10 cercariae per mouse by the tail immersion method; the mice were divided into two groups: untreated group and ginger-treated one. All mice were sacrificed at the end of 10th week post-infection. Worm recovery and egg counting in the hepatic tissues and faeces were determined. Surface topography of the recovered worms was studied by scanning electron microscopy. Histopathological examination of liver and intestine was done using routine histological procedures. The worm burden and the egg density in liver and faeces of mice treated with ginger were fewer than in non-treated ones. Scanning electron microscopical examination revealed that male worms recovered from mice treated with ginger lost their normal surface architecture, since its surface showed partial loss of tubercles' spines, extensive erosion in inter-tubercle tegumental regions and numerous small blebs around tubercles. Histopathological data indicated a reduction in the number and size of granulomatous inflammatory infiltrations in the liver and intestine of treated mice compared to non-treated mice. The results of the present work suggested that ginger has antischistosomal activities and provided a basis for subsequent experimental and clinical trials.

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“…Without doubt, major alterations arising out of PTX administration in rodents continue to be those observed previously in the liver (Reis et al, 2001;Xiong et al, 2003;El-Lakkany & Nosseir, 2007;Mati et al, 2010;Rabia et al, 2010). The liver is the organ where the immunological modulation process and its respective alterations on schistosomotic granulomata are more evident (Andrade & Warren, 1964;Carvalho et al, 1986;Silva et al, 2000;Tristão et al, 2000).…”

Section: Current Uses For Oograms In Experimental Schistosomiasismentioning

confidence: 93%

“…Additionally, in order to verify the number of eggs per female parasite in a day, the ratio of second-stage S. mansoni eggs per g intestine/number of female parasites recovered from the portal system was utilized (Pacheco et al, 2005(Pacheco et al, , 2008Mati et al, 2010). Female worms from the peritoneal cavity were not used in the analysis of the rates their eggs, laid in the peritoneum, produce free granulomata in situ that do not normally reach the intestine.…”

Section: Oogram Proceduresmentioning

confidence: 99%

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

Mati

Melo

2012

J. Helminthol.

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AbstractOogram methodology permitted a direct assessment of the fecundity ofSchistosoma mansoniand the passage of parasite eggs from mesenteric vessels into the intestinal lumen in AKR/J mice that had been infected via the intraperitoneal route with 60 cercariae of the trematode and later subjected to short-term subcutaneous treatments with pentoxifylline (PTX). The administration of PTX did not change oviposition kinetics, the individual fecundity of female parasites (as determined by the study of the ratio of second-stageS. mansonieggs per g intestine/number of female parasites recovered from the portal system), nor the number of mature and dead eggs retained in the host tissue, though the drug has known immunomodulatory effects, as shown previously in experimental schistosomiasis. A better appraisal was also carried out, including the study of statistical parameters, concerning the utilization of the ratios of each stage of immature eggs (first to fourth) per g intestine/number of female worms from the portal system. The second-stage eggs had the lowest variability, confirming that the utilization of this stage as an indicator of the individual fecundity of parasite females is indeed viable. In the light of our findings, current uses of oogram methodology are discussed. Moreover, additional consideration is given to data obtained in the present study concerning intraperitoneal infection withS. mansonicercariae in both untreated and treated mice of the AKR/J strain, such as the recovery of mature worms, eggs and free granulomas from the peritoneal cavity of these rodents.

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Effects of pentoxifylline duringSchistosoma mansoniinfection in Swiss mice: an analysis of worm burden, fecundity and liver histopathology

Cited by 11 publications

References 40 publications

Schistosoma mansoni: Antiparasitic effects of orally administeredNigella sativaoil and/orChroococcus turgidusextract

Schistosoma mansoni: Antiparasitic effects of orally administeredNigella sativaoil and/orChroococcus turgidusextract

Antischistosomal activity of ginger (Zingiber officinale) against Schistosoma mansoni harbored in C57 mice

Current applications of oogram methodology in experimental schistosomiasis; fecundity of femaleSchistosoma mansoniand egg release in the intestine of AKR/J mice following immunomodulatory treatment with pentoxifylline

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