Effects of PEGylated Fe–Fe3O4 core-shell nanoparticles on NIH3T3 and A549 cell lines

Domac, Begum Hira; Alkhatib, Sara; Zırhlı, Onur; Akdoğan, Nilay Gündüz; Dirican, Ş.C. Öçal; Bulut, Gülay; Akdoğan, Ozan

doi:10.1016/j.heliyon.2019.e03124

Cited by 13 publications

(3 citation statements)

References 20 publications

(26 reference statements)

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“…The presence of PEG brought stability and better biocompatibility to these nanoparticles and prevented toxic and pharmacokinetic effects of nanoparticles resulting from their interactions with cells or biological proteins. This suggests that the designed anticancer nanoparticle formulation is biocompatible with normal cells and would be very useful for targeting cancer cells without damaging/harming normal tissues [ 62 ].…”

Section: Resultsmentioning

confidence: 99%

Formulation and Characterization of Fe3O4@PEG Nanoparticles Loaded Sorafenib; Molecular Studies and Evaluation of Cytotoxicity in Liver Cancer Cell Lines

et al. 2023

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Iron oxide nanoparticles are one of the nanocarriers that are suitable for novel drug delivery systems due to low toxicity, biocompatibility, loading capacity, and controlled drug delivery to cancer cells. The purpose of the present study is the synthesis of coated iron oxide nanoparticles for the delivery of sorafenib (SFB) and its effects on cancer cells. In this study, Fe3O4 nanoparticles were synthesized by the co-precipitation method, and then sorafenib was loaded onto PEG@Fe3O4 nanoparticles. FTIR was used to ensure polyethylene glycol (PEG) binding to nanoparticles and loading the drug onto the nanoshells. A comparison of the mean size and the crystalline structure of nanoparticles was performed by TEM, DLS, and X-ray diffraction patterns. Then, cell viability was obtained by the MTT assay for 3T3 and HepG2 cell lines. According to FT-IR results, the presence of O–H and C–H bands at 3427 cm–1 and 1420 cm–1 peak correlate with PEG binding to nanoparticles. XRD pattern showed the cubic spinel structure of trapped magnetite nanoparticles carrying medium. The magnetic properties of nanoparticles were examined by a vibrating-sample magnetometer (VSM). IC50 values at 72 h for treatment with carriers of Fe3O4@PEG nanoparticle for the HepG2 cell line was 15.78 μg/mL (p < 0.05). This study showed that Fe3O4 nanoparticles coated by polyethylene glycol and using them in the drug delivery process could be beneficial for increasing the effect of sorafenib on cancer cells.

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Section: Resultsmentioning

confidence: 99%

Formulation and Characterization of Fe3O4@PEG Nanoparticles Loaded Sorafenib; Molecular Studies and Evaluation of Cytotoxicity in Liver Cancer Cell Lines

et al. 2023

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“…PLOS ONE •Sponges w/carboxymethyl chitosan and collagen peptides [167] •Gold nanoparticles (AuNP) [168] •Stromal vascular fraction (SVF) [169] •Quinone-based chromenopyrazole (QCP) antioxidant-laden silk fibroin electrospun nanofiber scaffold [170] •Pulmonary fibrosis associated RNA overexpression [171] •Titanium dioxide nanoparticle biofilm [172] •Neonatal cardiac fibroblasts infected with ETV2 [173] •exosomes platelet rich plasma [174] •tonsil derived Stem cell media [175] • [176] •Elaidic and linoleic (fatty acids) [119] •Light exposure [177] •Primary rat alveolar macrophages (AMO)-derived monocyte chemotactic protein-induced protein 1 (MCPIP1) knockdown [178] •Oxymatrine and Notch signaling pathway inhibitor (DAPT) via TGF-β1 [161]…”

Section: Drugsmentioning

confidence: 99%

Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing

et al. 2021

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The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then ‘scratched’ with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and proliferation in attempt to ‘heal’ the modeled wound. Keloid scars are known to have an accelerated wound closure phenotype in the scratch assay, representing an overactivation of wound healing. We performed a qualitative review of the recent literature searching for inhibitors of scratch assay activity that were already available in topical formulations under the hypothesis that such compounds may offer therapeutic potential in keloid treatment. Although several shortcomings in the scratch assay literature were identified, caffeine and allicin successfully inhibited the scratch assay closure and inflammatory abnormalities in the commercially available keloid fibroblast cell line. Caffeine and allicin also impacted ATP production in keloid cells, most notably with inhibition of non-mitochondrial oxygen consumption. The traditional Chinese medicine, shikonin, was also successful in inhibiting scratch closure but displayed less dramatic impacts on metabolism. Together, our results partially summarize the strengths and limitations of current scratch assay literature and suggest clinical assessment of the therapeutic potential for these identified compounds against keloid scars may be warranted.

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“…Afterward, the remaining samples were further cleaned by centrifugation and removal of the supernatant. This cleaning process was repeated at least 4 times [71][72][73].…”

Section: Fe Nanoparticle Synthesismentioning

confidence: 99%

Innovative technique for patterning Nd–Fe–B arrays and development of a microfluidic device with high trapping efficiency

Ozunlu¹,

Akdoğan²,

Bozkurt³

et al. 2021

Nanotechnology

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Trapping/separating bio-entities via magnetic field gradients created a vast number of possibilities to develop biosensors for the early detection of diseases without the need for expensive equipment or physician/lab technicians. Thus, opening a window for at-home disposable rapid test kits. In the scope of the current work, an innovative and cost-effective technique to form well-organized arrays of Nd–Fe–B patterns was successfully developed. High aspect ratio Nd–Fe–B flakes were synthesized by surfactant-assisted ball milling technique. Nd–Fe–B flakes were distributed and patterned into a PDMS matrix by the aforementioned technique. A microfluidic channel was integrated on the fabricated Nd–Fe–B/PDMS patch with a high magnetic field gradient to form a microfluidic device. Fe nanoparticles, suspended in hexane, were flowed through the microfluidic channel, and trapping of the magnetic nanoparticles was observed. More experiments would be needed to quantitatively study efficiency. Ergo, the microfluidic device with high trapping efficiency was developed. The established technique has the potential to outperform the precedents in trapping efficiency, cost, and ease of production. The developed device could be integrated into disposable test kits for the early detection of various diseases.

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Effects of PEGylated Fe–Fe3O4 core-shell nanoparticles on NIH3T3 and A549 cell lines

Cited by 13 publications

References 20 publications

Formulation and Characterization of Fe3O4@PEG Nanoparticles Loaded Sorafenib; Molecular Studies and Evaluation of Cytotoxicity in Liver Cancer Cell Lines

Formulation and Characterization of Fe3O4@PEG Nanoparticles Loaded Sorafenib; Molecular Studies and Evaluation of Cytotoxicity in Liver Cancer Cell Lines

Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing

Innovative technique for patterning Nd–Fe–B arrays and development of a microfluidic device with high trapping efficiency

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