Effects of PCBs on Liver Ultrastructure and Monooxygenase Activities in Japanese Quail

Stouvenakers, Nadine; Hugla, J. L.; Goffinet, Gérhard; Kremers, Pierre; Thomé, Jean-Pierre

doi:10.1007/s001289900122

Cited by 7 publications

(5 citation statements)

References 21 publications

(22 reference statements)

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“…Contrary to observations in mammals (Lipsky et al, 1978;Singh et al, 1996), birds (Williams et al, 1993;Stouvenakers et al, 1996), and catfish (Ictalurus punctatus) (Lipsky et al, 1978), no development of the SER was ob-served. This finding is in good agreement, however, with previous observations on PCB-contaminated carp (Cyprinus carpio) (Sivarajah et al, 1978) and DDT-contaminated zebrafish (Brachidanio rerio) (Weis, 1974).…”

Section: Liver Ultrastructurecontrasting

confidence: 68%

Effects of Polychlorinated Biphenyls on Liver Ultrastructure, Hepatic Monooxygenases, and Reproductive Success in the Barbel

Hugla

Thomé

1999

Ecotoxicology and Environmental Safety

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Section: Liver Ultrastructurecontrasting

confidence: 68%

Effects of Polychlorinated Biphenyls on Liver Ultrastructure, Hepatic Monooxygenases, and Reproductive Success in the Barbel

Hugla

Thomé

1999

Ecotoxicology and Environmental Safety

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“…We have chosen to use the Japanese quail as a representative for a bird species because some information on hepatic P450 induction for this species is available. For instance, the induction of hepatic P450 activities after treatment of Japanese quail with other organochlorine contaminants [15,16], ␤-naphthoflavone and phenobarbital [17,18], has been reported.…”

Section: Introductionmentioning

confidence: 99%

Induction of Hepatic Cytochrome P450 Activities by Toxaphene in Rat and Japanese Quail

Drenth

Oevelen

Buitenhuis

et al. 2000

Environ Toxicol Chem

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Abstract-Several organochlorine compounds can induce hepatic cytochrome P450 activities. We exposed female Japanese quail and female rats to a single oral dose of technical toxaphene ranging from 0.012 to 40 mg/kg body weight to investigate a possible dose-response relationship for P450 activity induction. The hepatic microsomal alkoxyresorufin dealkylation and steroid hydroxylation activities (testosterone, 17␤-estradiol) were determined. Only in the highest dose groups (40 mg/kg) of rats and Japanese quail were some P450 activities induced. Pentoxyresorufin-O-dealkylation, formation of 15␤-hydroxytestosterone, and formation of 2-hydroxyestradiol were induced significantly (p Յ 0.05) in rat hepatic microsomes with a factor of 3.9, 3.1, and 2.4, respectively. In Japanese quail hepatic microsomes, the formation rates of 6␤-, 15␣-, 16␤-hydroxytestosterone and androstenedione were induced significantly (p Յ 0.05) with a factor of 2.4, 6.5, 3.3, and 1.6, respectively. It is concluded that exposure to toxaphene can lead to specific P450 activity induction in rats and Japanese quail but only at doses near reported LD50 values. Because reported toxaphene levels in biota are lower compared with liver residue levels that we have measured in our study, we conclude that it is unlikely that P450 activity induction occurs in wildlife higher in the food chain due to environmental toxaphene exposure.

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“…In addition, the expressions and activities of enzymes have been reported to be affected by certain xenobiotics such as pesticides and aflatoxin in a dose-or time-dependent manner [15,17,22,23]. After exposure to organic chemicals such as aldicarb [24], carbamate insecticide, DDT [25], dioxin [26], PCB [27], and polycyclic aromatic hydrocarbons [28], an increase in hepatic MO activity in animals has been observed and studied [17,18]. Similarly, enhanced hepatic GST activity has been reported in rainbow trout (Oncorhynchus The analysis of VTG concentrations in the livers of wild male O. barbatulum individuals showed that those captured from the Ikawan River had the highest VTG concentrations (2.12 ± 0.66 µg PO 4 2+ /g protein), followed by those from Yusheng River (0.78 ± 0.25 µg PO 4 2+ /g protein) and Cijiawan River (0.59 ± 0.81 µg PO 4 2+ /g protein).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Xenoestrogen Status of Wuling Farm to Surrounding Water Bodies: An Application of Biochemical Parameters Using Onychostoma barbatulum

Liao¹,

Cheng

Wang

et al. 2022

JMSE

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The aim of this study was to apply biochemical parameters to Onychostoma barbatulum to understand whether Wuling Farm activities have caused organic pollution from xenoestrogens to flow into surrounding streams and thereby affected area aquatic ecosystems. Individuals of the Taiwan shovel-jaw carp (O. barbatulum) were collected by fyke netting in the Cijiawan, Kaoshan, and Yusheng Rivers in the protected area of Shei-Pa National Park and in the Ikawan River outside the protected areas from 4–5 June 2015 to 20–21 September 2015. The collected male individuals of O. barbatulum were divided into two groups. In the first group monooxygenase (Mon) activity, glutathione S-transferase (GST) activity, and vitellogenin (VTG) concentration in the liver were measured right after they were captured. Those in the second group were acclimated for 14 days and then exposed to 0 (control) or 10 ng/L 17β-estradiol for 10 days and then measured for changes in Mon, GST, and VTG in their livers. The value of VTG concentrations in O. barbatulum individuals collected from the Ikawan River was 2.12 ± 1.62 μg PO42+/g protein, which was the highest among all samples, followed by those collected from the Yusheng River (0.78 ± 1.00 μg PO42+/g protein). Individuals collected from the Yusheng River had the highest liver Mon activity (4.16 ± 1.08△A650/30 min/g protein) and the highest GST activity (1.58 ± 1.13 △A340/20 min/g protein), followed by those from the Ikawan River (Mon: 3.63 ± 1.13 △A650/30 min/g protein; GST: 1.24 ± 0.73 △A340/20 min/g protein). Comprehensive analyses showed that Mon and GST activities and VTG concentrations in livers measured right after being collected were lower than individuals exposed to 10 ng 17β-estradiol/L in the laboratory. Induced VTG concentrations were 9.87 ± 0.89 μg PO42+/g protein, Mon activity 8.02 ± 1.74 △A650/30 min/g protein, and GST activity 3.24 ± 0.62 △A340/20 min/g protein, indicating that farming activities have not significantly affected these aquatic organisms. However, pollution sources are still releasing pollutants containing xenoestrogens into the Yusheng River and Ikawan River. The impact of xenoestrogens is worthy of continuous long-term follow-up monitoring.

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Effects of PCBs on Liver Ultrastructure and Monooxygenase Activities in Japanese Quail

Cited by 7 publications

References 21 publications

Effects of Polychlorinated Biphenyls on Liver Ultrastructure, Hepatic Monooxygenases, and Reproductive Success in the Barbel

Effects of Polychlorinated Biphenyls on Liver Ultrastructure, Hepatic Monooxygenases, and Reproductive Success in the Barbel

Induction of Hepatic Cytochrome P450 Activities by Toxaphene in Rat and Japanese Quail

Xenoestrogen Status of Wuling Farm to Surrounding Water Bodies: An Application of Biochemical Parameters Using Onychostoma barbatulum

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