Effects of Polychlorinated Biphenyls on Liver Ultrastructure, Hepatic Monooxygenases, and Reproductive Success in the Barbel

Hugla, J. L.; Thomé, Jean-Pierre

doi:10.1006/eesa.1998.1761

Cited by 49 publications

(27 citation statements)

References 60 publications

(79 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In female chub, hepatic EROD activity was significantly higher in individuals sampled in the BKME and PCB contaminated sites than in both reference sites. This is in agreement with the EROD inducing activity of paper mill effluents (Soimasuo et al, 1995;Coakley et al, 2001) and PCB (Addison et al, 1982;Hugla and Thomé, 1999). Moreover, EROD activity in females from the pesticide contaminated site was higher than in chubs from reference 1, revealing the exposure of fish to EROD inducer(s).…”

Section: Biochemical Measurementssupporting

confidence: 86%

Multiscale approach of fish responses to different types of environmental contaminations: A case study

Mayon

Bertrand

Leroy

et al. 2006

Science of The Total Environment

View full text Add to dashboard Cite

In order to study the responses of wild fish to environmental contaminations, different sites (two references and three contaminated) were sampled across the Walloon hydrographical network (southern Belgium). The status of fish communities was characterized according to an index of biotic integrity (IBI). Furthermore, population structure, reproductive parameters and biochemical assays were performed on chub (Leuciscus cephalus) as sentinel species. The results showed impaired responses in fish sampled downstream paper mill effluents (BKME), in a polychlorinated biphenyls (PCB) contaminated site, and in a river receiving pesticides (i.e. atrazine and hexachlorocyclohexane). High level of hepatic 7-ethoxyresorufin-Odeethylase (EROD) activity were evidence of direct toxicant exposure, while population structures showed unbalanced size-frequency distributions. Moreover, in the pesticide contaminated site, a higher proportion of atretic oocytes was found in females and abnormally high plasma alkali-labile phosphorus (ALP) values were noticed in males, compared to reference sites, suggesting that chubs were exposed to estrogenic compounds. IBI scores from both sites were also lower than in reference sites. On the other hand, no particular response was found in hepatic glutathione-S-transferase (GST) activities, hepatic heat-shock protein HSP70 concentrations and plasma sex steroids (estradiol-17β, 11-ketotestosterone and testosterone) in chubs from any sites. By using a multiparametric approach, a better understanding of response mechanisms to pollution in fish may be achieved in order to improve the ecological status of river ecosystems.

show abstract

Section: Biochemical Measurementssupporting

confidence: 86%

Multiscale approach of fish responses to different types of environmental contaminations: A case study

Mayon

Bertrand

Leroy

et al. 2006

Science of The Total Environment

View full text Add to dashboard Cite

show abstract

“…For instance, barbel has been shown to be more strongly contaminated by polychlorinated biphenyls (PCBs) than salmonids and other cyprinids from the same locations (Keck & Raffenot 1979, Vindimian et al 1991, Hugla et al 1995. Hugla et al (1995) correlated alterations in liver ultrastructure similar to our observations to high PCB concentrations in barbel from the River Meuse, and PCBs have also been shown to generate a multitude of hepatic alterations in other studies (Weibel & Paumgartner 1978, Hugla et al 1996, Hugla & Thomé 1999. Indeed, Keiter et al (2008) documented considerably higher concentrations of PCBs in sediments from Sites Rottenacker and Ehingen than in those from Site Riedlingen.…”

Section: Reasons For Fish Decline In the Danube Riversupporting

confidence: 84%

“…3) was basically identical and resembled the description of liver of control barbel Barbus barbus as described by Hugla & Thomé (1999). Overall, the appearance of the liver of control fish showed a remarkable homogeneity between individual hepatocytes.…”

Section: Control Fishsupporting

confidence: 81%

“…The most obvious ultrastructural alterations observed in the hepatocytes of field fish from Rottenacker and Ehingen were proliferation, modified structure and irregular distribution of almost any cell organelle system, as well as a drastic reduction of glycogen contents, eventually resulting in a complete loss of cellular compartmentation. Similar cellular changes were observed in several studies investigating the health status of different fish species from polluted streams (Hugla et al 1995, Schramm et al 1998, Gernhöfer et al 2001), or exposed to contaminated sediments (Wu et al 1999) as well as various xenobiotics (Oulmi et al 1995, Braunbeck & Appelbaum 1999, Hugla & Thomé 1999, Triebskorn et al 2004. Generally, most of the liver reactions observed can be interpreted as symptoms of elevated stress of fish during mobilization of energy reserves and as evidence of morphological restructuring in adaptation to the need for intensified metabolization/detoxification capacities (Segner & Braunbeck 1998, Triebskorn et al 2004.…”

Section: Ultrastructural Investigationssupporting

confidence: 77%

“…Proliferation of lysosomes and myelin-like structures has repeatedly been interpreted as a sign of general intoxication caused by xenobiotics (Hinton et al 1978, Braunbeck 1993 related to an increased degeneration of cell organelles (Hugla & Thomé 1999). Alterations of the RER, including proliferation, fragmentation, and vesiculation, are common reactions to xenobiotic stress (Hacking et al 1978, Burkhardt-Holm et al 1999, Wu et al 1999) and have been correlated to a higher biotransformation capacity of hepatocytes (Schoor & Couch 1979, Braunbeck & Völkl 1993, Au et al 1999.…”

Section: Ultrastructural Investigationsmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of fish health status in the Upper Danube River by investigation of ultrastructural alterations in the liver of barbel Barbus barbus

Grund¹,

Keiter²,

Böttcher³

et al. 2010

Dis. Aquat. Org.

View full text Add to dashboard Cite

Despite intensive efforts and tightened guidelines for improvement of water quality over the last 2 decades, declines of fish populations have been reported for several rivers around the world. The present study forms part of a comprehensive weight-of-evidence approach, which aims to identify potential causes for the decline in fish catches observed in the Upper Danube River. The major focus of the present study is the investigation of the health status of wild barbel Barbus barbus L. collected from 3 locations along the Danube River, which experienced different levels of contamination. Whereas the comparison of the condition factor (CF) of field fish with that of control fish revealed no differences, ultrastructural investigations indicated severe disturbance of hepatic cell metabolism in field fish from the more contaminated sites Rottenacker and Ehingen, compared to both control fish and field fish from the less contaminated site Riedlingen. The ultrastructural analysis provided information about reactions of e.g. the rough endoplasmic reticulum, peroxisomes, and mitochondria, indicating an impaired health status of barbel at the sampling sites Rottenacker and Ehingen. Even though a straightforward cause-effect relationship between sediment contamination and ultrastructural alterations could not be established, based on a meta-analysis and toxicity assays it may be suggested that sediment-bound xenobiotics at least partly account for the hepatocellular changes. A relationship between impaired fish health status and the decline of fish catches along the Upper Danube River cannot be excluded.

show abstract

Polychlorinated biphenyl tissue‐concentration thresholds for survival, growth, and reproduction in fish

Berninger

Tillitt

2019

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Polychlorinated biphenyls (PCBs) have left a legacy of environmental contamination. Even though they were banned from production and active use in the 1970s, they persist in the environment and still have the potential to impact aquatic life. Our objective was to identify data from controlled laboratory studies of PCB‐related adverse effects in fish and to conduct a meta‐analysis on mortality, growth, and reproductive (MGR) threshold responses. For each endpoint type, we compiled data on the lowest‐observed‐adverse effect concentration (LOAEC) and the degree of effect at the LOAEC as a percentage of control. The LOAECs were expressed as tissue concentrations, so the term lowest‐observed‐adverse‐effect residue concentration (LOAER) was used to represent PCB exposures. The lower limit of applicability was set at 0.1 μg/g total PCB tissue concentration, below which adverse MGR effects in fish were not supported by the data. Sensitivity distributions identifying the probability of adverse effects in fish populations or communities predicted that 25% of fish species would be impacted between 0.1 and 7.5 μg/g. Concentration–response threshold regressions were developed from the MGR datasets. For example, a 1 μg/g total PCB tissue concentration would predict effects of 17% mortality, 15% growth, and 39% reproductive. The analysis determined the degree of adverse response, with uncertainty estimates, expected across a broad range of PCB tissue exposure concentrations in fish. Data generated from MGR endpoints were combined to determine an approach for overall effect thresholds for PCB‐related injury in fish. The MGR datasets included only laboratory data; however, responses were compared with field‐observed effects. The present review provides a comprehensive assessment of PCB‐induced injury in fish utilizing a data‐inclusive approach. Environ Toxicol Chem 2019;38:712–736. Published 2018 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.

show abstract

Effects of Polychlorinated Biphenyls on Liver Ultrastructure, Hepatic Monooxygenases, and Reproductive Success in the Barbel

Cited by 49 publications

References 60 publications

Multiscale approach of fish responses to different types of environmental contaminations: A case study

Multiscale approach of fish responses to different types of environmental contaminations: A case study

Assessment of fish health status in the Upper Danube River by investigation of ultrastructural alterations in the liver of barbel Barbus barbus

Polychlorinated biphenyl tissue‐concentration thresholds for survival, growth, and reproduction in fish

Contact Info

Product

Resources

About