Effects of ozone oxidative preconditioning on different hepatic biomarkers of oxidative stress in endotoxic shock in mice

Abstract

In endotoxic shock, variations are known to occur in different biochemical parameters of oxidative stress. Ozone oxidative preconditioning (OOP) is a good candidate to restore the redox balance on different tissue. This investigation examined the effect of OOP on different biomarkers of oxidative stress in hepatic tissue of mice treated with lipopolysaccharide (LPS). LPS doses of 30 mg/kg were administered intraperitoneally (i.p.) and pretreatment with ozone/oxygen mixture (OOM) was applied i.p. at 0.2, 0.4, and 1.2 mg/kg once daily during 5 days before LPS injection. The mice were euthanized under ether atmosphere at different times, 1 and 24 h after LPS injection. Hepatic tissue from all animals was taken for biochemical determinations of oxidative stress parameters such as thiobarbituric acid reactive substances (TBARS) content and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione S-transferase (GST). The results demonstrated that OOP reduces levels of TBARS content and increases the activity of GPx in hepatic tissue. In conclusion, OOP was able to recover the redox balance and in this way to protect the animals against the oxidative damage induced by endotoxemia.

Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders

“…Increase dose-dependent manner : glutathione peroxidase (GPx). ( Rodriguez et al, 2011 ) Preconditioning : 0.2, 0.4, 1.2 mg/kg intraperitoneally, once daily, for 5 days, before lipopolysaccharide (LPS) injection (0.1 mg/kg). Dexamethasone (30 mg/kg) used as a reference drug.…”

Ozone preconditioning attenuates contrast-induced nephropathy in rats

“…Ozone therapy can induce tolerance to ischemic insults and attenuate ischemiaereperfusion (I/R) injury in various tissues including the kidney [11e13]. This phenomenon is known as ozone oxidative preconditioning (OOP) and has been proposed to protect the organs against oxidative stress [14,15].…”

Is the increased ozone dosage key factor for its antiinflammatory effect in an experimental model of mesenteric ischemia?

“…Recently, ozone, an antioxidant that induces protective enzymes, has been studied in many conditions, such as peritonitis. [15] In addition, the beneficial effects of ozone therapy have recently been studied under various ischemic conditions, such as ovarian, testicular, and skeletal muscle injuries caused by tourniquets. [16][17][18] Based on the anti-inflammatory and antioxidant effects of ozone therapy, we aimed to evaluate the beneficial effect of ozone therapy in mesenteric ischemia in a rat model.…”