Effects of ozone and relative humidity on fluorescence spectra of octapeptide bioaerosol particles

Pan, Yong; Santarpia, Joshua L.; Ratnesar-Shumate, Shanna; Corson, Elizabeth; Eshbaugh, Jonathan P.; Hill, Steven C.; Williamson, Chatt; Coleman, Mark A.; Bare, Christopher; Kinahan, Sean

doi:10.1016/j.jqsrt.2013.09.017

Cited by 26 publications

(30 citation statements)

References 41 publications

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“…Less is known about the ability of the WIBS to detect bacteria, which are predicted to be another important source of PBAP with global emissions estimated to be between 0.74 and 28.1 Tg/yr [Burrows et al, 2009a;Hoose et al, 2010a;Jacobson and Streets, 2009]. Emission spectra of several kinds of bacteria have been measured at a variety of excitation wavelengths [Hill et al, 2009;Pan et al, 2014b], as a function of particle size, illumination intensity, and fluorophore concentration [Hill et al, 2001] and as a function of oxidation and humidity [Pan et al, 2014a]. Bacteria typically have strong absorption features around 280 nm with emission occurring between 310 and 400 nm.…”

Section: Methodsmentioning

confidence: 99%

Airborne observations of regional variation in fluorescent aerosol across the United States

et al. 2015

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Airborne observations of fluorescent aerosol were made aboard an airship during CloudLab, a series of flights that took place in September and October of 2013 and covered a wideband of longitude across the continental U.S. between Florida and California and between 28 and 37 N latitudes. Sampling occurred from near the surface to 1000 m above the ground. A Wideband Integrated Bioaerosol Sensor (WIBS-4) measured average concentrations of supermicron fluorescent particles aloft (1 μm to 10 μm), revealing number concentrations ranging from 2.1 ± 0.8 to 8.7 ± 2.2 × 10 4 particles m À3 and representing up to 24% of total supermicron particle number. We observed distinct variations in size distributions and fluorescent characteristics in different regions, and attribute these to geographically diverse bioaerosol. Fluorescent aerosol detected in the east is largely consistent with mold spores observed in a laboratory setting, while a shift to larger sizes associated with different fluorescent patterns is observed in the west. Fluorescent bioaerosol loadings in the desert west were as high as those near the Gulf of Mexico, suggesting that bioaerosol is a substantial component of supermicron aerosol both in humid and arid environments. The observations are compared to model fungal and bacterial loading predictions, and good agreement in both particle size and concentrations is observed in the east. In the west, the model underestimated observed concentrations by a factor between 2 and 4 and the prescribed particle sizes are smaller than the observed fluorescent aerosol. A classification scheme for use with WIBS data is also presented.

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Section: Methodsmentioning

confidence: 99%

Airborne observations of regional variation in fluorescent aerosol across the United States

et al. 2015

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“…Furthermore, fluorescence is subject to interferences from non-biological aerosols that contain aromatic hydrocarbons (e.g., industrial chemicals and engine exhausts). Laboratory studies have shown that LIF spectra of PBAP can be strongly influenced by growth conditions or agglomeration with leftover growth media or other materials (Pan et al 2014b;Sivaprakasam et al 2011), or by exposure to ultraviolet light and ozone (Pan et al 2014a). Using multiple excitation wavelengths can improve discrimination and reduce false positives.…”

Section: Real-time Techniquesmentioning

confidence: 99%

Real-time sensing of bioaerosols: Review and current perspectives

Huffman

Perring

Savage

et al. 2019

Aerosol Science and Technology

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178

138

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Detection of bioaerosols, or primary biological aerosol particles (PBAPs), has become increasingly important for a wide variety of research communities and scientific questions. In particular, real-time (RT) techniques for autonomous, online detection and characterization of PBAP properties in both outdoor and indoor environments are becoming more commonplace and have opened avenues of research. With advances in technology, however, come challenges to standardize practices so that results are both reliable and comparable across technologies and users. Here, we present a critical review of major RT instrument classes that have been applied to PBAP research, especially with respect to environmental science, allergy monitoring, agriculture, public health, and national security. Eight major classes of RT techniques are covered, including the following: (i) fluorescence spectroscopy, (ii) elastic scattering, microscopy, and holography, (iii) Raman spectroscopy, (iv) mass spectrometry, (v) breakdown spectroscopy, (vi) remote sensing, (vii) microfluidic techniques, and (viii) paired aqueous techniques. For each class of technology we present technical limitations, misconceptions, and pitfalls, and also summarize best practices for operation, analysis, and reporting. The final section of the article presents pressing scientific questions and grand challenges for RT sensing of PBAP as well as recommendations for future work to encourage high-quality results and increased cross-community collaboration.

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“…In the cases listed at 150 ppb, the ozone increased from approximately 0 ppb to 150 ppb in the first approximately 30 min. The spectra have been weighted by particle diameter as 1/d 2.05 for 263-nm excitation and as 1/d 2.8 for 351-nm excitation, and so the averaged spectra shown are the converted counts from the measurements of the ICCD that is estimated to be if all particles were 1 μm diameter [75].…”

Section: The Evolution Of Optical Property Of Bioaerosols In Atmosphementioning

confidence: 99%

“…These six algorithms use more of the information successively measured by the DPFS regarding the fluorescence intensities, fluorescence spectral shapes and elastic scattering, and have an ability to discriminate against an increasingly larger fraction of the atmospheric aerosol background in the case of particles having known fluorescence spectra. However, the more specific system, the lower positive alarm rate, but the more negative false alarm rate, in which particular particle that has small optical property variation, e.g., the same bioaerosol under slightly different growth conditions [51] or atmospheric environments [75], could be missed by detection.…”

Section: Algorithms For Discriminating Particular Bioaerosol From Atmmentioning

confidence: 99%

Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Pan

2015

Journal of Quantitative Spectroscopy and Radiative Transfer

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115

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a b s t r a c tThis paper offers a brief review on the detection and characterization of biological and other organic-carbon (OC) aerosol particles in atmosphere using laser-induced-fluorescence (LIF) signatures. It focuses on single individual particles or aggregates in the micron and supermicron size range when they are successively drawn through the interrogation volume of a point detection system. Related technologies for these systems that have been developed in last two decades are also discussed. These results should provide a complementary view for studying atmospheric aerosol particles, particularly bioaerosol and OC aerosol particles from other analytical technologies.Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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Effects of ozone and relative humidity on fluorescence spectra of octapeptide bioaerosol particles

Cited by 26 publications

References 41 publications

Airborne observations of regional variation in fluorescent aerosol across the United States

Airborne observations of regional variation in fluorescent aerosol across the United States

Real-time sensing of bioaerosols: Review and current perspectives

Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

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