Abstract:a b s t r a c tThis paper offers a brief review on the detection and characterization of biological and other organic-carbon (OC) aerosol particles in atmosphere using laser-induced-fluorescence (LIF) signatures. It focuses on single individual particles or aggregates in the micron and supermicron size range when they are successively drawn through the interrogation volume of a point detection system. Related technologies for these systems that have been developed in last two decades are also discussed. These … Show more
“…Previously it has been reported that real-time LIF analysis enables classification of bioaerosols from other aerosols in atmosphere (Gabey et al 2010;Huffman et al 2010;Pan 2015). Our results show that combined LIF and LIBS analysis is promising tool for online classification of atmospheric bioaerosols.…”
Section: Identification Of Microbial Particlessupporting
confidence: 54%
“…Fluorescence spectra or fluorescence band (single or multiple) intensity measurement can be utilized to discriminate bioaerosols from other aerosols (P€ ohlker et al 2012;Pan 2015). Fluorescence-based online bioaerosol instruments, such as BioScout, WIBS, and UV-APS, have been used in various environments, but it is really challenging to classify different types of bioaerosols with these instruments (Gabey et al 2010;Huffman et al 2010;Saari et al 2014).…”
Section: Lif Spectra Of Microbial Particlesmentioning
Online characterization of fungal and bacterial spores is important in various applications due to their health and climatic relevance. The aim of this study was to demonstrate the capability of the combination of electro-dynamic balance assisted laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF) techniques for the online detection of single fungal spores (Aspergillus versicolor and Penicillium brevicompactum) and bacteria (Bacillus aureus). The method enabled sensitive and repeatable LIBS analysis of common elemental components (Ca, Na, and K) from single microbial particles for the first time. Significant differences in the concentrations of these elements were observed between the species, e.g., bacterial spores had over three orders of magnitude higher Ca concentration (2 £ 10 ¡12 g/particle) compared to fungal spores (3-5 £ 10 ¡16 g/particle). The LIF analysis has previously been used to distinguish bioaerosols from other aerosols due to their fluorescence ability. This study showed that combination of LIF and LIBS analysis is a promising tool for identification of different bioaerosol particle types.
EDITORPaul J. Ziemann
“…Previously it has been reported that real-time LIF analysis enables classification of bioaerosols from other aerosols in atmosphere (Gabey et al 2010;Huffman et al 2010;Pan 2015). Our results show that combined LIF and LIBS analysis is promising tool for online classification of atmospheric bioaerosols.…”
Section: Identification Of Microbial Particlessupporting
confidence: 54%
“…Fluorescence spectra or fluorescence band (single or multiple) intensity measurement can be utilized to discriminate bioaerosols from other aerosols (P€ ohlker et al 2012;Pan 2015). Fluorescence-based online bioaerosol instruments, such as BioScout, WIBS, and UV-APS, have been used in various environments, but it is really challenging to classify different types of bioaerosols with these instruments (Gabey et al 2010;Huffman et al 2010;Saari et al 2014).…”
Section: Lif Spectra Of Microbial Particlesmentioning
Online characterization of fungal and bacterial spores is important in various applications due to their health and climatic relevance. The aim of this study was to demonstrate the capability of the combination of electro-dynamic balance assisted laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF) techniques for the online detection of single fungal spores (Aspergillus versicolor and Penicillium brevicompactum) and bacteria (Bacillus aureus). The method enabled sensitive and repeatable LIBS analysis of common elemental components (Ca, Na, and K) from single microbial particles for the first time. Significant differences in the concentrations of these elements were observed between the species, e.g., bacterial spores had over three orders of magnitude higher Ca concentration (2 £ 10 ¡12 g/particle) compared to fungal spores (3-5 £ 10 ¡16 g/particle). The LIF analysis has previously been used to distinguish bioaerosols from other aerosols due to their fluorescence ability. This study showed that combination of LIF and LIBS analysis is a promising tool for identification of different bioaerosol particle types.
EDITORPaul J. Ziemann
“…Для быстрого обнаружения и идентификации патогенных биологических частиц респирабельной (задерживаемой в органах дыхания человека) фракции 1-10 мкм может быть применен проточно-оптический метод (ПОМ) анализа, использующий измерения флюоресценции и рассеяния света от от-дельных частиц аэрозоля [6][7][8]. В ПОМ отбор пробы и освещение частиц происходит в потоке воздуха, что позволяет анализировать до 10 4 отдельных частиц аэрозоля в секунду и выдавать результат анализа за время, не превышающее нескольких минут.…”
unclassified
“…Задача осложняется тем, что био-аэрозольный фон атмосферы -динамичная система, флуоресцентные свойства которой могут меняться в естественных условиях при воздействии солнечного света, температуры, влажности и других факторов [8]. Таким образом, задача правильной идентификации патогенных частиц в составе аэрозоля с помощью ПОМ требует повышения селективности метода.…”
unclassified
“…Таким образом, задача правильной идентификации патогенных частиц в составе аэрозоля с помощью ПОМ требует повышения селективности метода. В существующих устройствах ПОМ для повышения селективности включают в оптическую систему дополнительные источники излучения и (или) фотоде-текторы [2,7,8]. Например, применяют дополнительный источник возбуждения в области длин волн 330-380 нм, что позволяет, помимо спектра флуоресценции триптофана, регистрировать спектры флуо-ПРИМЕНЕНИЕ МЕТОДОВ ХЕМОМЕТРИКИ ДЛЯ АНАЛИЗА БИОАЭРОЗОЛЕЙ … Научно-технический вестник информационных технологий, механики и оптики, 2016, том 16, № 1 32 ресценции NADH и флавинов в границах спектрального диапазона 400-600 нм [7].…”
The airborne, satellite, and balloon‐borne instrumentation is a powerful tool for the measurement of microphysical properties and the chemical composition of aerosol and cloud particles. Such instruments can be subdivided into two broad categories: remote sensing instrumentation and in situ instrumentation. In the first category, there are dynamic instrumentation like cloud RADARs (radio detection and ranging), LIDARs (light detection and ranging), and backscattersondes. In contrast, in the second category, there are passive instruments like multispectral and hyperspectral instruments for zenith measurements or limb/occultation detections. Both categories can determine the microphysical properties of the aerosols while the second category can, although only with complex a priori assumptions, also determine their chemical composition. This article is an updated discussion on different types of such airborne aerosol instruments, considering their detection limits and ranges, reliability, and applications. The paper also briefly describes the different platforms available for airborne aerosol/satellite instrumentation along with different payload configurations.
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