Effects of Oxymatrine on the apoptosis of human esophageal carcinoma Eca109 cell line and its mechanism

Jin, Yi; Hu, Jianli; Wang, Qiong; Li, Zhenyu; Chen, Yeshan

doi:10.1007/s11596-008-0319-y

Cited by 16 publications

(17 citation statements)

References 3 publications

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“…16 OMT induces the apoptosis of esophageal carcinoma cells related to the upregulation of p21 and downregulation of p-ERK1/2 and CyclinD1, and contributes to apoptotic cell death of pancreatic cancer via regulation of Bcl-2 and IAP families, release of mitochondrial cytochrome c and activation of caspase-3. 17 But, whether OMT affects the HIF-1a signaling and hemangiomagenesis is uncertain. In the present study, OMT significantly reduced cell proliferation and induced apoptosis followed by blockade of HIF-1a signaling, and OMT combined with other agents such as NM-3 exerts synergistic inhibitory effect on cancer cells, 18 suggesting that OMT inhibition of HIF-1a signaling might provide a novel therapeutic strategy for the treatment of HA.…”

Section: Discussionmentioning

confidence: 99%

Oxymatrine suppresses proliferation and induces apoptosis of hemangioma cells through inhibition of HIF-1a signaling

et al. 2015

Int J Immunopathol Pharmacol

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Oxymatrine (OMT), a natural quinolizidine alkaloid, has been known to have anti-inflammation, anti-anaphylaxis, and chemopreventive effects on various cancer cells. To clarify the underlying role and molecular mechanisms of OMT in human hemangioma (HA), in the present study, we examined the expression of hypoxia-inducible factor-1a (HIF-1a) and vascular endothelial growth factor (VEGF) in different phases of human HA. After HA derived endothelial cells (HDEC) were pretreated with different concentrations of OMT, cell proliferation, apoptosis, and cycle distribution were evaluated by MTT assay and flow cytometry analysis, respectively. The effects of OMT on expression of HIF-1a signaling were determined by real-time PCR and western blot assays. Our results showed that, the expression of HIF-1a and VEGF was significantly increased in proliferating phase HA, but decreased in involuting phase HA. Moreover, OMT in vitro inhibited proliferative activities and induced cell apoptosis and cycle arrest in G 0 /G 1 phase in HA cells with decreased expression of HIF-1a, VEGF, Bcl-2, and CyclinD1, and increased expression of p53. Taken together, our findings suggest that, the expression of HIF-1a and VEGF is increased in proliferating phase HA, and OMT suppresses cell proliferation and induces cell apoptosis and cycle arrest in proliferative phase HA through inhibition of the HIF-1a signaling pathway, suggesting OMT may provide a novel therapeutic strategy for the treatment of HA.

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Section: Discussionmentioning

confidence: 99%

Oxymatrine suppresses proliferation and induces apoptosis of hemangioma cells through inhibition of HIF-1a signaling

et al. 2015

Int J Immunopathol Pharmacol

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“…6,[21][22][23] However, little is known about its effects on human hepatoma, and the precise mechanism involved remains largely unknown. Therefore, in this study, we proved the anticancer effects of oxymatrine on human hepatoma Hep-G2 and SMMC-7721 cells and explored the related molecular mechanisms.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Effects of Oxymatrine on the Proliferation and Apoptosis of Human Hepatoma Carcinoma Cells

Liu

Dai

et al. 2015

Technol Cancer Res Treat

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Oxymatrine, one of the main active components of extracts from the dry roots of Sophora flavescens, has been reported to possess anticancer activities in vitro and in vivo. However, the precise mechanism involved remains largely unknown. The present study is conducted to investigate the anticancer activity and the underlying mechanisms of oxymatrine in human hepatoma cells in vitro and in vivo. Hep-G2 and SMMC-7721 cells were treated by oxymatrine and subjected to methyl thiazolyl tetrazolium analysis, Hoechst 33342 staining, annexin V/propidium iodide double staining, reverse transcription polymerase chain reaction, and Western blot analysis. In addition, SMMC-7721 xenograft tumors were established in male nude BALB/c mice, and oxymatrine was intravenously administered to evaluate the anticancer capacity in vivo. Our results showed that oxymatrine inhibited the proliferation and induced apoptosis of Hep-G2 and SMMC-7721 cells in a dose-dependent manner in vitro. Furthermore, the RNA and protein expression of Bax and caspase 3 levels were significantly upregulated, whereas the expression of Bcl-2 was downregulated. These protein interactions may play a pivotal role in the regulation of proliferation and apoptosis. More importantly, our in vivo studies showed that administration of oxymatrine decreased tumor growth in a dosedependent manner. Immunohistochemistry analysis demonstrated an increase of Bax and caspase 3 and a decrease of Bcl-2 in tumor tissues following oxymatrine treatment which are consistent with the in vitro results. Taken together, our findings indicated that oxymatrine can inhibit cell proliferation and induce apoptosis of human hepatoma Hep-G2 and SMMC-7721 cells and might offer a therapeutic potential advantage for human hepatoma chemoprevention or chemotherapy.

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“…More and more attention was paid to its anti-tumor effects via inducing apoptosis of carcinoma [12,19,20]. However, the mechanism of oxymatrine in cancer treatment is still not very clear.…”

Section: Discussionmentioning

confidence: 99%

“…Oxymatrine also has been demonstrated to have the anti-tumor effects including induction of apoptosis and cell cycle arrest [11], inhibition of cell cycle progression following DNA damage in carcinoma cells [12], and suppression of xenografted human gastric cancer cells SGC-7901 growth in vivo [13]. Since, to date, very few treatments have targeted cancer stem-like cells, the detection of a side population of CSCs in breast cancer leads us to investigate whether oxymatrine might affect the side population specifically.…”

Section: Introductionmentioning

confidence: 99%

Oxymatrine diminishes the side population and inhibits the expression of β-catenin in MCF-7 breast cancer cells

et al. 2010

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Cancer stem cells (CSCs) play a critical role in both cancer initiation and relapse as they are resistant to most cytotoxic agents and able to proliferate indefinitely. The plant alkaloid oxymatrine has many biological activities including the ability to induce cell cycle arrest and apoptosis, which makes it a potentially useful agent for targeting cancer cells. In order to determine whether it has beneficial pharmacological properties to eradicate CSCs, we analyzed the effects of oxymatrine on MCF-7 breast cancer cells. Cancer stem-like cells' (side population, SP) identification and sorting were performed. The inhibitory effect of oxymatrine was evaluated on the sorted SP and non-SP cells. The results indicated that oxymatrine caused a dose-dependent reduction in the proliferation of MCF-7 cells and a decrease in SP cells. Wnt/β-catenin signaling pathway was also examined by analyzing the expression of total β-catenin and phosphorylated β-catenin in cytoplasm, and the results showed that the growth inhibitory effects of oxymatrine treatment on MCF-7 cells may be due to the inhibition of SP and Wnt/β-catenin signaling pathway. Further work is warranted to explore whether oxymatrine may be a useful novel therapeutic drug for targeting breast CSCs.

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Effects of Oxymatrine on the apoptosis of human esophageal carcinoma Eca109 cell line and its mechanism

Cited by 16 publications

References 3 publications

Oxymatrine suppresses proliferation and induces apoptosis of hemangioma cells through inhibition of HIF-1a signaling

Oxymatrine suppresses proliferation and induces apoptosis of hemangioma cells through inhibition of HIF-1a signaling

RETRACTED: Effects of Oxymatrine on the Proliferation and Apoptosis of Human Hepatoma Carcinoma Cells

Oxymatrine diminishes the side population and inhibits the expression of β-catenin in MCF-7 breast cancer cells

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