Effects of oxymatrine on proliferation and apoptosis in human hepatoma cells

Song, Guanbin; Luo, Qing; Qin, Jian; Wang, Lu; Shi, Yisong; Sun, Caixin

doi:10.1016/j.colsurfb.2005.12.012

Cited by 74 publications

(56 citation statements)

References 17 publications

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“…In this study, we found that BLM instillation induced a marked increase in lipid peroxidation as reflected by the MDA level, and this level was significantly decreased by OM treatment in a dose-dependent manner. We postulate that OM has antioxidant activities due to the hydroxyl group in its structure (36). MPO activity has been used as a marker of neutrophil influx into the tissue (37).…”

Section: Discussionmentioning

confidence: 99%

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway

Liu

Lü

et al. 2012

Int J Mol Med

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Abstract. Oxymatrine (OM) is an alkaloid extracted from the Chinese herb Sophora flavescens Ait. with a variety of pharmacological activities. The aim of this study was to investigate the preventive effects of OM on bleomycin (BLM)-induced pulmonary fibrosis (PF) and to further explore the underlying mechanisms. C57BL/6 mice were randomly assigned to five groups: the saline sham group; the BLM group, in which mice were endotracheally instilled with BLM (3.0 mg/kg); and the BLM plus OM groups, in which OM was given to mice daily (10, 20 or 40 mg/kg) one day after BLM instillation for 21 days. The bronchoalveolar lavage fluid (BALF) and lung tissues were collected at 15 and 22 days post BLM administration, respectively. Lung tissues were stained with hematoxylin and eosin (H&E) for histological evaluation. Levels of tumor necrosis factor (TNF)-α, interleukin-6 (IL-6) and nitric oxide (NO) in mouse BALF were measured, as well as myeloperoxidase (MPO) activity and malondialdehyde (MDA) content in lung homogenates. The inducible nitric oxide synthase (iNOS) expression in the lung tissues was determined by immunohistochemical staining, quantitative real-time PCR and western blot analysis. Moreover, the expression of transforming growth factor (TGF)-β1, Smad2, Smad3, p-Smad2 and p-Smad3 were also detected. We found that OM improved BLM-induced lung pathological changes, inhibited MPO activity and reduced MDA levels in a dose-dependent manner. OM also dose-dependently inhibited the release of TNF-α and IL-6, and decreased the expression of iNOS in lung tissues and thus prevented NO release in response to BLM challenge. In addition, OM decreased the expression of TGF-β1, p-Smad2 and p-Smad3, which are all important members of the TGF-β/ Smad signaling pathway. Our study provides evidence that OM significantly ameliorated BLM-induced PF in mice via the inhibition of iNOS expression and the TGF-β/Smad pathway.

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Section: Discussionmentioning

confidence: 99%

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway

Liu

Lü

et al. 2012

Int J Mol Med

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“…The downregulation of the bcl-2 gene and the upregulation of the p53 gene were also observed in human hepatoma cells after treatment with oxymatrine. A significant cell cycle blockage in the G 2 /M and S phases was detected in oxymatrine-treated human hepatoma SMMC-7721 www.nature.com/aps Wang W et al Acta Pharmacologica Sinica npg cells, which might explain the inhibition of cell proliferation [26] .…”

Section: Anti-cancer Effects Of Oxymatrinementioning

confidence: 99%

“…The size of SP cells was suppressed, and the primary genes of the Wnt signaling pathway, such as β-catenin, cyclinD1, and c-Myc, were downregulated after treatment with CKI. This may also explain how CKI can attenuate the side effects of chemotherapy; chemotherapeutic drugs only inhibit non-SP cells (differentiated cells), not cancer stem-like cells (SP cells) [26] . In another study, CKI had an anti-inflammatory effect, protecting against carcinogen-induced oxidative damage, which inhibits rat gastric carcinogenesis [27] .…”

Section: Studies On Compound Kushen Injectionmentioning

confidence: 99%

Anti-tumor activities of active ingredients in Compound Kushen Injection

Wang¹,

et al. 2015

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Kushen (Radix Sophorae Flavescentis) has a long history of use for the treatment of tumors, inflammation and other diseases in traditional Chinese medicine. Compound Kushen Injection (CKI) is a mixture of natural compounds extracted from Kushen and Baituling (Rhizoma Smilacis Glabrae). The main principles of CKI are matrine (MT) and oxymatrine (OMT) that exhibit a variety of pharmacological activities, including anti-inflammatory, anti-allergic, anti-viral, anti-fibrotic and cardiovascular protective effects. Recent evidence shows that these compounds also produce anti-cancer actions, such as inhibiting cancer cell proliferation, inducing cell cycle arrest, accelerating apoptosis, restraining angiogenesis, inducing cell differentiation, inhibiting cancer metastasis and invasion, reversing multidrug resistance, and preventing or reducing chemotherapy-and/or radiotherapy-induced toxicity when combined with chemotherapeutic drugs. In this review, we summarize recent progress in studying the anti-cancer activities of MT, OMT and CKI and their potential molecular targets, which provide clues and references for further study.

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“…Oxymatrine markedly and dose-dependently inhibits the proliferation of cancer cells 38 by inhibiting the expression of β-catenin, 39 blocking cell cycle in G2/M and S phase, and regulating Bcl-2 and p53 expression. 40 …”

Section: Anti-proliferationmentioning

confidence: 99%

“…38 It downregulates anti-apoptotic Bcl-2 and inhibitor of apoptosis protein (IAP) families, upregulates pro-apoptotic Bax expression, releases mitochondrial cytochrome c, and activates caspase-3/-9 in human cancer cells. 41,42 Livin and Survivin are members of IAPs, which can play important roles in inhibiting apoptosis by exerting their negative action on caspases. 43 Overexpression of Livin and Survivin is found associated with poor prognosis in primary and cultured tumors, but oxymatrine obviously downregulates Livin and Survivin expression, thus promoting an apoptosis effect.…”

Section: Apoptosis-promotingmentioning

confidence: 99%

Oxymatrine and cancer therapy

Zhang

et al. 2015

Eur J Inflamm

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Oxymatrine is a kind of alkaloid extracted from traditional Chinese herb Sophora flavescens Ait. It has been proved to exert various biological activities such as anti-angiogenesis, proliferation-inhibiting, apoptosis-promoting, analgesicstrengthening, and anti-metastasis. The biological activities are related with inhibition of angiogenesis-associated factors, regulation of related signaling pathway and protein expression, synergistic effects with chemotherapy drug, cell cycle arrest and inhibition of voltage-activated K+ channel. In this review, we summarize the recent investigations of oxymatrine in cancer therapy so as to provide references for further study and clinical therapy.

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Effects of oxymatrine on proliferation and apoptosis in human hepatoma cells

Cited by 74 publications

References 17 publications

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway

Anti-tumor activities of active ingredients in Compound Kushen Injection

Oxymatrine and cancer therapy

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