Effects of osmotic pressure, acid, or cold stresses on antibiotic susceptibility of Listeria monocytogenes

Al‐Nabulsi, Anas A.; Osaili, Tareq M.; Shaker, Reyad R.; Olaimat, Amin N.; Jaradat, Ziad W.; Elabedeen, Noor A. Zain; Holley, Richard A.

doi:10.1016/j.fm.2014.07.015

Cited by 90 publications

(61 citation statements)

References 38 publications

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“…Listeria monocytogenes from food sources have generally been reported to be highly susceptible to ampicillin, gentamycin, penicillin, rifampicin and vancomycin. On the other hand, varying prevalence of resistance to tetracycline by L. monocytogenes isolates from different food sources have also been reported [16,53,61,62]. The high number of L. monocytogenes isolates showing intermediate susceptibility to ciprofloxacin (11.3%) and clindamycin (17.7%) has similarly been reported by Lyon et al [63] and Bertsch et al [16].…”

Section: Antibiotic Resistance Among L Monocytogenes Isolatesmentioning

confidence: 66%

Prevalence and Characteristics of Listeria monocytogenes Isolates in Raw Milk, Heated Milk and Nunu, a Spontaneously Fermented Milk Beverage, in Ghana

et al. 2018

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Listeria monocytogenes is a gram-positive food-borne pathogen that causes listeriosis in humans. Currently, there is little information on the prevalence of Listeria monocytogenes in raw milk and traditional yoghurt-like milk beverage, nunu, in Ghana. The purpose of this study was to investigate the prevalence of L. monocytogenes isolates in raw cow milk, boiled milk and nunu in Ghana, and to characterize these L. monocytogenes isolates according to their serogroups, virulence potentials and antibiotic susceptibility profiles. A total of 254 samples comprising 114 raw cow milk, 56 boiled milk and 84 nunu were collected from dairy farms and market vendors for detection of L. monocytogenes. The overall prevalence of L. monocytogenes in raw milk, boiled milk and nunu was 5.5% (14/254). Listeria monocytogenes was prevalent in raw cow milk (8.8%; 10/114) and nunu (13.1%; 11/84), while no Listeria spp. was not detected in boiled milk. A total of 62 L. monocytogenes isolates were analysed to belong to molecular serogroups 1/2a-3a (32/62, 51.6%), 1/2b-3b-7 (14/62, 22.6%), 4b-4d-4e (9/62, 14.5%) and 1/2c-3c (7/62, 11.3%). All 62 L. monocytogenes isolates harbored the virulence-associated genes inlA, inlB, inlC, inlJ, plcA, actA, hlyA, iap and prfA. All Listeria monocytogenes in the present study were generally susceptible to the tested antibiotics, except neomycin and tetracycline, for which phenotypic resistance was observed among isolates.

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Section: Antibiotic Resistance Among L Monocytogenes Isolatesmentioning

confidence: 66%

Prevalence and Characteristics of Listeria monocytogenes Isolates in Raw Milk, Heated Milk and Nunu, a Spontaneously Fermented Milk Beverage, in Ghana

et al. 2018

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“…In food production environments microorganisms undergo a variety of stresses including extreme temperatures, which can have a significant effect on bacterial survival during food processing thus impacting the efficacy of decontamination treatments (Delaquis and Bach, 2012). For example, Al-Nabulsi et al (2015) demonstrated an increased resistance of L. monocytogenes to antibiotic treatment when cells were exposed to temperature decreased to 10°C. In this study, the stress encountered from the first 24 h storage cycle in the cold environment could lead to the increased resistance to ACP treatment.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Cold plasma inactivation of internalised bacteria and biofilms for Salmonella enterica serovar Typhimurium, Listeria monocytogenes and Escherichia coli

Ziuzina¹,

Han²,

Cullen

et al. 2015

International Journal of Food Microbiology

160

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Microbial biofilms and bacteria internalised in produce tissue may reduce the effectiveness of decontamination methods. In this study, the inactivation efficacy of in-package atmospheric cold plasma (ACP) afterglow was investigated against Salmonella Typhimurium, Listeria monocytogenes and Escherichia coli in the forms of planktonic cultures, biofilms formed on lettuce and associated bacteria internalised in lettuce tissue. Prepared lettuce broth (3%) was inoculated with bacteria resulting in a final concentration of ~7.0 log 10 CFU/ml. For biofilm formation and internalisation, lettuce pieces (5 x 5 cm) were dip-inoculated in bacterial suspension of ~7.0 log 10 CFU/ml for 2 h and further incubated for 0, 24 and 48 h at either 4°C or room temperature (~22°C) in combination with light/dark photoperiod or at 4°C under dark conditions. Inoculated samples were sealed inside a rigid polypropylene container and However, the existence of biofilms and internalised bacteria should be considered for further optimisation of ACP treatment parameters in order to achieve an effective control of the realistic challenges posed by foodborne pathogens.

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“…The alteration of the metabolites agrees with the changes of the corresponding gut microbial functions based on the COGs analysis as described above, revealing that the metabolic abilities of amino acid, energy, lipid and carbohydrate were inhibited by TET administration. This study proposed that TET treatment influenced the mouse metabolism by changing the gut microbial community structure and function and disturbing the food digestion and absorption (Cho et al 2012;Al-Nabulsi et al 2015).…”

Section: Alteration Of Metabolic Profiles In Mouse Serummentioning

confidence: 99%

Metagenomic insights into tetracycline effects on microbial community and antibiotic resistance of mouse gut

Yin

Zhang

et al. 2015

Ecotoxicology

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Antibiotics have been widely used for disease prevention and treatment of the human and animals, and for growth promotion in animal husbandry. Antibiotics can disturb the intestinal microbial community, which play a fundamental role in animals' health. Misuse or overuse of antibiotics can result in increase and spread of microbial antibiotic resistance, threatening human health and ecological safety. In this study, we used Illumina Hiseq sequencing, (1)H nuclear magnetic resonance spectroscopy and metagenomics approaches to investigate intestinal microbial community shift and antibiotic resistance alteration of the mice drinking the water containing tetracycline hydrochloride (TET). Two-week TET administration caused reduction of gut microbial diversity (from 194 to 89 genera), increase in Firmicutes abundance (from 24.9 to 39.8%) and decrease in Bacteroidetes abundance (from 69.8 to 51.2%). Metagenomic analysis showed that TET treatment affected the intestinal microbial functions of carbohydrate, ribosomal, cell wall/membrane/envelope and signal transduction, which is evidenced by the alteration in the metabolites of mouse serum. Meanwhile, in the mouse intestinal microbiota, TET treatment enhanced the abundance of antibiotic resistance genes (ARGs) (from 307.3 to 1492.7 ppm), plasmids (from 425.4 to 3235.1 ppm) and integrons (from 0.8 to 179.6 ppm) in mouse gut. Our results indicated that TET administration can disturb gut microbial community and physiological metabolism of mice, and increase the opportunity of ARGs and mobile genetic elements entering into the environment with feces discharge.

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Effects of osmotic pressure, acid, or cold stresses on antibiotic susceptibility of Listeria monocytogenes

Cited by 90 publications

References 38 publications

Prevalence and Characteristics of Listeria monocytogenes Isolates in Raw Milk, Heated Milk and Nunu, a Spontaneously Fermented Milk Beverage, in Ghana

Prevalence and Characteristics of Listeria monocytogenes Isolates in Raw Milk, Heated Milk and Nunu, a Spontaneously Fermented Milk Beverage, in Ghana

Cold plasma inactivation of internalised bacteria and biofilms for Salmonella enterica serovar Typhimurium, Listeria monocytogenes and Escherichia coli

Metagenomic insights into tetracycline effects on microbial community and antibiotic resistance of mouse gut

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