Effects of organic solvents on activity and stability of lipases produced by thermotolerant fungi in solid-state fermentation

Hernández-Rodríguez, Blanca Elizabeth; Córdova, Jesús; Bárzana, Eduardo; Favela‐Torres, Ernesto

doi:10.1016/j.molcatb.2009.06.004

Cited by 62 publications

(45 citation statements)

References 54 publications

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“…The results demonstrated that the lipase can be successfully used for the synthesis of ethyl oleate, with high etherification rates and substrate conversion, over short reaction periods under conditions when ethanol is in excess. Similarly, Hernandez-Rodriguez et al (2009) showed that Downloaded by[New York University] at 03:to the lipase produced by Rhizopus sp., the enzyme produced by the thermophilic fungus Rhizomucor sp. through SSF can be used in the ethyl oleate synthesis reaction.…”

mentioning

confidence: 88%

From Wastes to High Value Added Products: Novel Aspects of SSF in the Production of Enzymes

El-Bakry

Abraham

Cerda

et al. 2015

Critical Reviews in Environmental Science and Technology

107

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Solid state fermentation (SSF), a process that occurs in the absence or near absence of water, has been used for the production of various high value added products such as enzymes and other organic components. This paper reviews the recent studies reported on the use of SSF for the production of enzymes; lipases, proteases, cellulases, hemicellulases, ligninases, glucoamylases, pectinases and inulinases. The microorganisms used for fermentation are mostly fungi and substrates are waste materials from the agriculture and food industry. This shows the advantages of SSF from an economical and environmental viewpoint. The paper provides an Downloaded by [New York University] at 03:19 04 February 2015 ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT 2 update on several issues, viz. wastes, microorganisms and scale-up and control of the process of fermentation in solid-state.

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mentioning

confidence: 88%

From Wastes to High Value Added Products: Novel Aspects of SSF in the Production of Enzymes

El-Bakry

Abraham

Cerda

et al. 2015

Critical Reviews in Environmental Science and Technology

107

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“…14 Since polar organic solvents have technological advantages, such as low toxicity, low boiling points, low costs and offer the possibility of using polar substrates for novel reactions, the method proposed by Pencreac'h and Baratti was modified in the present and used to compare the activity of crude P. aphidis lipase in aqueous media and in various, polar, water-miscible organic solvents (Table II). 2 The activity of P. aphidis lipase towards p-NPP in an aqueous medium was considered as 100 %. Among the organic solvents, lipase activity towards p-NPP was only observed in 1088 DIMITRIJEVIĆ et al acetone and acetonitrile (Table II), giving values of R O/A of 0.006 and 0.002, respectively.…”

Section: Lipase Activity In Aqueous Medium and In Organic Solvents Tomentioning

confidence: 99%

“…3 Biocatalysis in organic solvents offers several advantages over biocatalysis in aqueous media, due to the increased solubility of hydrophobic compounds, the ability to perform new reactions which are kinetically or thermodynamically restricted in water, suppression of undesirable side reactions, control or modification of enzyme selectivity, the possibility of recovery of some products by employment of low-boiling-point organic solvents and an increased enzyme thermostability in organic solvents. 2,4 Although enzymes exhibit many advantages, they do not always meet the desired levels of activity, productivity and, most importantly, stability in organic solvents. 5 Generally, hydrophobic solvents lead to higher enzymatic activity and stability than hydrophilic solvents, which strip water required for the enzymatic function, and lower the catalytic activity.…”

Section: Introductionmentioning

confidence: 99%

“…For these reasons, it is necessary to find lipases that are active and stable in polar organic solvents. 2 A considerable number of bacterial and fungal lipases have been commercially produced, the latter being preferable because fungi generally produce extracellular enzymes, which facilitate recovery of the enzyme from the fermentation broth. 7 Pseudozyma (Candida) antarctica is one of the most important sources of lipases for industrial applications.…”

Section: Introductionmentioning

confidence: 99%

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Production of lipase from Pseudozyma aphidis and determination of the activity and stability of the crude lipase preparation in polar organic solvents

Dimitrijević

Veličković

Bezbradica³

et al. 2011

JSCS

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The production of lipase from Pseudozyma aphidis (DSM 70725) was determined in six different media. The highest lipase production was observed in a medium with glucose as the sole carbon source, and yeast extract and sodium nitrate as the nitrogen sources. The time course studies of growth and lipase production in the optimal medium revealed that the highest lipase production was achieved at the end of the log phase of growth, reaching the value of 35.0 U cm -3 in the fifth day of cultivation. The effects of various polar, water-miscible, organic solvents on the activity and stability of the crude lipase produced by P. aphidis were evaluated. The hydrolytic activity of the crude lipase towards p-nitrophenyl palmitate (p-NPP) in aqueous media and in organic solvents was determined, using the same spectrophotometric assay in both the aqueous and organic media. The crude lipase preparation exhibited activity towards p-NPP only in acetone and acetonitrile, while the lipase was stable only in acetone, with 23 % residual activity after 24 h of incubation. These results suggested that lipase from P. aphidis can be used as a biocatalyst for potential applications in such organic solvents.

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“…Recently, lipase has attracted significant interest for commercial production, as it has several advantages, including the fact that no cofactor is required, it is active in the interfaces of organic compounds, and facilitates a wide range of substrates (Hernández-Rodríguez et al, 2009). Therefore, lipase has many practical applications in industry, including as a biocatalyst for biodiesel synthesis, in bio-detergents, fine chemicals, waste water treatment, food additives, pharmaceuticals, leather processing and biomedical assays (Salihu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Production of Dry Extract Lipase from Pseudomonas Aeruginosa by the Submerged Fermentation Method in Palm Oil Mill Effluent

Hermansyah¹,

Maresya²,

Putri³

et al. 2018

IJTech

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Palm Oil Mill Effluent (POME) is an agro-industrial waste product with high availability and which contains high quantities of organic compounds that are necessary for microbial growth. Cultures of Pseudomonas aeruginosa were grown in POME to produce lipase using the submerged fermentation method. The objective of this study is to obtain the optimum value of lipase activity produced by the cultures of Pseudomonas aeruginosa using POME as the substrate through the submerged fermentation method and to obtain the dry extract of lipase. In the study, the one factor at a time (OFAT) method was applied, which allowed observation of the effect of inoculum and additional nutrient concentrations, such as Ca 2+ ion, olive oil, peptone and Tween 80, on the activity of lipase. These factors were investigated in shake flask fermentation at 30C over 96 hours. The activity unit of lipase was determined by the titrimetric reaction of olive oil hydrolysis using crude lipase. The optimum value of the lipase activity unit (1.327 U/mL) was gained when 3% (v/v) of inoculum, 4 mM of Ca 2+ ion, 0.4% (v/v) of olive oil, 0.9% (m/v) of peptone, and 0.9% of Tween 80 were added into the medium. Crude lipase was then dried using a spray dryer. Subsequently, 15.643 g of dry extract lipase was obtained from 500 mL of cell free supernatant. In further research, the lipase activity assay would be better achieved using the p-nitrophenyl palmitate hydrolysis method and examined by a spectrophotometer.

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Effects of organic solvents on activity and stability of lipases produced by thermotolerant fungi in solid-state fermentation

Cited by 62 publications

References 54 publications

From Wastes to High Value Added Products: Novel Aspects of SSF in the Production of Enzymes

From Wastes to High Value Added Products: Novel Aspects of SSF in the Production of Enzymes

Production of lipase from Pseudozyma aphidis and determination of the activity and stability of the crude lipase preparation in polar organic solvents

Production of Dry Extract Lipase from Pseudomonas Aeruginosa by the Submerged Fermentation Method in Palm Oil Mill Effluent

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