Effects of Oral Administration of Antioxidant Taurine on Haematological Parameters in Wistar Rats

Abstract

The present study evaluates the effects of oral administration of taurine on haematological parameters in normal wistar rats. Tissue oxidative stress is implicated in pathogenesis of various clinical disorders and antioxidant taurine is emerging as prophylactic and therapeutic agent. However, studies on effects of taurine on normal physiology are not reported in literature. Normal saline (Group I) or 5% taurine in normal saline was administered in dose of 50 mg (Group II), 250 mg (Group III) or 500 mg kg(-1) of b.wt. (Group IV) through intragastric intubation for 60 days. The blood cell counts, haemoglobin content, packed cell volume, blood indices, bleeding time and clotting time were estimated using routine laboratory haematological techniques. Neutrophil's phagocytic activity was determined by nitroblue tetrazolium reduction test; serum lysozyme activity was estimated colorimetrically by the degree to lyse bacterial cell suspension and serum taurine levels were estimated by HPLC fluorimetric technique. Platelet count showed a decrease in Group III and IV when compared with Group I and II (p<0.001). Mean corpuscular haemoglobin of Group III and IV are significantly lowered when compared to Group I (p<0.001). A statistically significant decrease was observed in the mean corpuscular haemoglobin concentration between Group 1 and Group IV (p<0.001). The neutrophil percentage of Group II, Group III and Group IV showed a significant increase over Group I (p<0.001). The percentage of lymphocytes showed a significant decrease in Group II, III and Group IV when compared to Group I (p<0.001). Neutrophil's phagocytic activity is significantly lowered in Group III and IV when compared to Group I (p<0.001). The serum lysozyme activity of Group III and IV showed a significant increase over Group I (at p<0.001). From the present study it may be concluded that long term oral administration of taurine affects normal haematological functions.

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“…group also showed reduced values of PCV. This is similar to the study carried out by [12] , when it was discovered that PCV values in Wistar rats decreased when the rats were exposed to a substance (Taurine), for a long time. It was discovered in a research [13] that diazepam has effects on the cardiovascular makeup of the rats, in that it inhibits blood flow.…”

Effect of vitamin B12 and taurine on the alleviation of nutritional anaemia in common sole (Solea solea)

“…Moreover, taurine levels in mussel are even three times higher, while mussel and ragworm are equally capable to alleviate anaemia in sole (Kals, Blonk, Mheen, et al, ). According to Anand et al (), Salze and Davis (), Sirdah et al () and Takagi et al (), fish being deficient of taurine are associated with decreasing Hct levels (haemolytic anaemia). Looking at the taurine levels analysed in mussel, it is unlikely that the “high” taurine level chosen in the current study is too high for sole.…”

“…The requirement of taurine for sole is, as far as we know, hitherto unknown. A deficiency of taurine can affect Hct and Hb levels (Anand, Rajakumar, Felix, & Balasubramanian, ; Salze & Davis, ; Sirdah, El‐Agouza, & Abu Shahla, ; Takagi et al, ). Ragworm contains ±7 g kg −1 dm (own analysis), mussel contains ±25 g kg −1 dm (Spitze, Wong, Rogers, & Fascetti, ), and the commercial pellet tested ±3.5 g kg −1 dm (Table ), of taurine.…”

Effect of Long-Term Peroral Taurine Injection on Blood Indexes of Rats

“…Ключові слова: таурин, кров, щурі, глюкоза, швидкість оксигенації гемоглобіну, лактатдегідрогеназа, еритроцити, гемоглобін Відомо, що таурин використовується у харчовій промисловості в дозах 5-20 мг на літр чи кілограм продукту і що його споживання не є строго регламентованим [5] і може мати як позитивний, так і негативний впливи на організм людини і тварин. Виявлено, що високі дози таурину за тривалого введення порушують гомеостаз крові у щурів.…”

“…Виявлено, що високі дози таурину за тривалого введення порушують гомеостаз крові у щурів. Так, за перорального введення таурину протягом 30-60 діб у дозах 40-500 мг/кг знижується вміст гемоглобіну у крові та в одному еритроциті [5], однак зростає кількість еритроцитів [3], що може спричинити порушення дихальної функції крові. За тривалого перорального введення доз 250 та 500 мг/кг знижується кількість тромбоцитів і лімфоцитів, але збільшується кількість нейтрофілів, що вказує на зміни у захисних функціях крові.…”