Effects of Obesity on Gingival Oxidative Stress in a Rat Model

Tomofuji, Takaaki; Yamamoto, Tatsuo; Tamaki, Naofumi; Ekuni, Daisuke; Azuma, Tetsuji; Sanbe, Toshihiro; Irie, Koichiro; Kasuyama, Kenta; Umakoshi, Michihiro; Murakami, Jun; Kokeguchi, Susumu; Morita, Manabu

doi:10.1902/jop.2009.080621

Cited by 39 publications

(53 citation statements)

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“…[1][2][3] Animal studies also revealed that the gingiva in obese rats exhibited higher densities of polymorphonuclear leukocytes than that in normal rats. 4,5 Thus, obesity may be a risk factor for periodontal diseases.…”

Section: Introductionmentioning

confidence: 99%

“…9 Recent clinical studies suggested that increased oxidative stress and/or decreased anti-oxidative status are involved in the progression of periodontal diseases. [10][11][12] In a rat model, gingival oxidative stress was associated with periodontal inflammation 4 and osteoclast differentiation on alveolar bone surface. 13 Therefore, a systemic increase in ROS following obesity may lead to progression of periodontal inflammation and osteoclast differentiation through gingival oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…In the periodontal tissue, the polymorphonuclear leukocytes per standard area (0.05 mm × 0.1 mm) were counted in the connective tissues subjacent to the junctional epithelium. 4 The distance between the cemento-enamel junction and alveolar bone crest was also measured to evaluate the level of alveolar bone resorption. 4 TRAP-positive osteoclasts occurring along the edge of the alveolar bone surface were counted and reported as number/mm.…”

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confidence: 99%

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Effects of exercise training on gingival oxidative stress in obese rats

Azuma

Tomofuji

Endo

et al. 2011

Archives of Oral Biology

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Objective: The purpose of the present study was to investigate the effects of exercise training on serum reactive oxygen species (ROS) level and gingival oxidative stress in obese rats fed a high-fat diet.Design: Rats were divided into three groups (n=14/group): one control group (fed a regular diet) and two experimental groups (fed a high-fat diet with and without exercise training [treadmill: 5 days/week]). The rats were sacrificed at 4 or 8 weeks. The level of serum reactive oxidative metabolites (ROM) was measured as an indicator of circulating ROS. The level of 8-hydroxydeoxyguanosine (8-OHdG) and reduced-form glutathione (GSH)/oxidized-form glutathione (GSSG) ratio were determined to evaluate gingival oxidative stress. Results:The obese rats fed a high-fat diet without exercise training showed higher serum ROM levels [Carratelli Units (CARR U)] (mean ±SD; 413 ±64) than the control (333 ±12) at 4 weeks (p= 0.023). Such a condition resulted in higher 8-OHdG levels (ng/mg mtDNA) (0.97 ±0.18) (p< 0.05) and a lower GSH/GSSG ratio (17.0 ±3.1) (p< 0.05) in gingival tissues, compared to the control (0.55 ±0.13 for 8-OHdG and 23.6 ±5.8 for GSH/GSSG ratio) at 8 weeks. In addition, the obese rats fed a high-fat diet with exercise training showed lower serum ROM (623 ±103) (p< 0.001) and gingival 4 8-OHdG levels (0.69 ±0.17) (p= 0.012) than those without exercise training (1105 ±95 for ROM and 0.55 ±0.13 for 8-OHdG) at 8 weeks. Conclusions:Obesity prevention by exercise training may effectively suppress gingival oxidative stress by decreasing serum ROS in rats.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of exercise training on gingival oxidative stress in obese rats

Azuma

Tomofuji

Endo

et al. 2011

Archives of Oral Biology

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“…The inflammation of the supracrestal gingival connective tissue between the molars in each specimen at consecutive sections or levels 10 and 20 was examined based on multiple parameters, including apical migration of the junctional epithelium (JE), rete ridge elongation, interdental crestal alveolar bone resorption, lymphocyte numbers, and blood vessel density (14,57). The number of lymphocytes per unit area (0.05 mm by 0.05 mm) was counted in the area of the JE and adjacent connective tissue (14,57,61,62). The apical migration of the JE, elongation of rete ridges, and resorption of alveolar bone was measured using ImageScope software with a microgrid at a magnification of ϫ200.…”

Section: Vol 78 2010 P Gingivalis Serb In Rat Bone Loss 4561mentioning

confidence: 99%

“…The apical migration of the JE, elongation of rete ridges, and resorption of alveolar bone was measured using ImageScope software with a microgrid at a magnification of ϫ200. The distances from the CEJ to the coronal portion of the connective tissue attachment (apical migration), from the CEJ to the apical portion of the rete ridge, and from the CEJ to the level of the alveolar bone crest were measured (14,57,61,62).…”

Section: Vol 78 2010 P Gingivalis Serb In Rat Bone Loss 4561mentioning

confidence: 99%

Role of Porphyromonas gingivalis Phosphoserine Phosphatase Enzyme SerB in Inflammation, Immune Response, and Induction of Alveolar Bone Resorption in Rats

Bainbridge¹,

Verma²,

Eastman³

et al. 2010

Infect Immun

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Porphyromonas gingivalis secretes a serine phosphatase enzyme, SerB, upon contact with gingival epithelial cells in vitro. The SerB protein plays a critical role in internalization and survival of the organism in epithelial cells. SerB is also responsible for the inhibition of interleukin-8 (IL-8) secretion from gingival epithelial cells infected with P. gingivalis. This study examined the ability of a P. gingivalis SerB mutant to colonize the oral cavity and induce gingival inflammation, immune responses, and alveolar bone resorption in a rat model of periodontal disease. Both P. gingivalis ATCC 33277 and an isogenic ⌬SerB mutant colonized the oral cavities of rats during the 12-week experimental period. Both of the strains induced significant (P < 0.05) systemic levels of immunoglobulin G (IgG) and isotypes IgG1, IgG2a, and IgG2b, indicating the involvement of both T helper type 1 (Th1) and Th2 responses to infection. Both strains induced significantly (P < 0.05) higher levels of alveolar bone resorption in infected rats than in sham-infected control rats. However, horizontal and interproximal alveolar bone resorption induced by the SerB mutant was significantly (P < 0.05) lower than that induced by the parental strain. Rats infected with the ⌬SerB mutant exhibited significantly higher levels of apical migration of the junctional epithelium (P < 0.01) and polymorphonuclear neutrophil (PMN) recruitment (P < 0.001) into the gingival tissues than rats infected with the wild type. In conclusion, in a rat model of periodontal disease, the SerB phosphatase of P. gingivalis is required for maximal alveolar bone resorption, and in the absence of SerB, more PMNs are recruited into the gingival tissues.

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Influence of the treatment of periodontal disease in serum concentration of sirtuin 1 and mannose‐binding lectin

Caribé

Villar

Romito

et al. 2020

Journal of Periodontology

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BackgroundIncreased levels of periodontal pathogens disrupt the homeostasis between the host and its microbiota and increase susceptibility to periodontal diseases. Periodontitis increases the serum concentration of mannose‐binding lectin (MBL), which exacerbates local inflammatory processes. In animal studies, sirtuin 1 (SIRT1) was associated with protection against inflammation. This study analyzed the influence of non‐surgical periodontal treatment on serum levels of MBL and SIRT1.MethodsForty patients with periodontitis and 38 periodontally healthy individuals (aged 45 to 79 years) were included. Periodontitis patients received scaling and root planing using machine driven and hand instruments. Clinical parameters, inflammatory biomarkers, MBL, and SIRT1 levels were measured at baseline and at post‐treatment.ResultsFor all patients, an inverse correlation was observed between serum concentrations of MBL and SIRT1 (r = ‐0.30; P = 0.006). Periodontal treatment reduced serum concentrations of MBL (1,099.35 ± 916.59 to 861.42 ± 724.82 ng/mL; P < 0.001) and C‐reactive protein (6.05 ± 8.99 to 2.49 ± 2.89 mg/L; P = 0.009). By contrast, SIRT1 serum levels increased (1.06 ± 1.03 to 1.66 ± 1.64 ng/mL; P < 0.001) following periodontal treatment.ConclusionsPeriodontal treatment was associated with decreased serum concentrations of MBL and CRP and increased serum levels of SIRT1. Prospective studies are needed to assess the impact of these biomarkers on pathophysiology of periodontitis.

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Effects of Obesity on Gingival Oxidative Stress in a Rat Model

Abstract: Obesity induced gingival oxidative stress with increasing serum reactive oxygen metabolites in rats. In the periodontal lesion, gene expressions related to a capacity for xenobiotic detoxification were downregulated in the obese model.

Cited by 39 publications

References 21 publications

Effects of exercise training on gingival oxidative stress in obese rats

Effects of exercise training on gingival oxidative stress in obese rats

Role of Porphyromonas gingivalis Phosphoserine Phosphatase Enzyme SerB in Inflammation, Immune Response, and Induction of Alveolar Bone Resorption in Rats

Influence of the treatment of periodontal disease in serum concentration of sirtuin 1 and mannose‐binding lectin

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