Effects of nutrients on the production of AK-111-81 macrolide antibiotic by Streptomyces hygroscopicus

Gesheva, Victoria; Иванова, В.; Gesheva, R

doi:10.1016/j.micres.2004.06.005

Cited by 78 publications

(55 citation statements)

References 14 publications

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“…In some marine ecosystems, such as the deep sea floor and coral reefs, experts estimate that the biological diversity is higher than in the tropical rainforests (Edward et al, 2006). As marine environmental conditions are extremely different from terrestrial ones, it is surmised that marine actinomycetes have different characteristics from those of terrestrial counterparts, and therefore, might produce different types of bioactive compounds (Fenical et al, 1999;Gesheva et al, 2005). The present study deals with screening for the isolation of actinomycetes to produce antibiotics, isolated from the marine sediments in and around Gokharna and Murudeshwara coastal regions and determination of their antimicrobial activity.…”

Section: Introductionmentioning

confidence: 99%

Screening, isolation and purification of antibacterial agents from marine actinomycetes

Attimarad¹,

Ediga

Karigar³

et al. 2012

Int Curr Pharm J

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As marine environmental conditions are extremely different from terrestrial ones, it is surmised that marine actinomycetes might produce novel bioactive compounds. Hence marine sediments, collected from the coastal areas of Gokharna and Muradeshwara of Karnataka state, were screened Seventeen isolates were obtained on starch-casein agar media by soil dilution technique. However, only six isolates namely ACT-A2, ACT-A3, ACT-A4, ACT-A5, ACT-A7 and ACT-A15 showed significant antibacterial activity against both gram-positive and gram-negative bacteria. Morphological, cultural and biochemical characterization indicated that the isolates belong to Streptomyces genus of Actinomycetes. Further studies were carried out with the most active ACT-A2. Optimization of media, temperature and pH by shake flask fermentation indicated starch-casein, 28°C and 7 to be suitable for ACT-A2. The production of antibiotics began after 24 h, reached maximum at 72 h and maintained at the same level up to 120 h. Ethyl acetate was used to extract antibacterial compounds from the culture filtrate. TLC was done on silica gel using ethyl acetate: methanol (6:4) and direct bioautography has shown the presence of two active substance, one with Rf 0.8 has more activity than the other with Rf 0.4. Further purification is done by column chromatography using a mixture of dicholoromethane and ethyl acetate. The findings from this investigation reveal that the strain ACT-A2 in order exhibited superior antimicrobial activities to other sediment isolates of actinomycetes.DOI: http://dx.doi.org/10.3329/icpj.v1i12.12448 International Current Pharmaceutical Journal 2012, 1(12): 394-402

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Section: Introductionmentioning

confidence: 99%

Screening, isolation and purification of antibacterial agents from marine actinomycetes

Attimarad¹,

Ediga

Karigar³

et al. 2012

Int Curr Pharm J

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“…Basak and Majumdar (1975) reported that kanamycin production by Streptomyces kanamyceticus ATCC 12853 required magnesium sulfate and potassium phosphate (0.4 and 1.0 g L −1 , respectively) and Fe and Zn (0.25 and 0.575 mg mL −1 , respectively), amounts of Mn and Ca did not have any effect, and Cu, Co, Ni, and V have inhibitory effect. Divalent ions as Mn 2+ , Cu 2+ , Fe 2+ stimulated AK-111-81 antibiotic biosynthesis by Streptomyces hygroscopicus 111-81 (Gesheva et al, 2005). The divalent metal ions (Mg, Fe and Mn) sodium dihydrogen phosphate were found essential for bacitracin production by Bacillus licheniformis, whereas Na 2 SO 4 and CaCl 2 decreased the bacitracin yield (Yousaf, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Tanaka et al (2010) made a connection between the rare earth elements scandium and lanthanum and increased activation of the expression of nine genes belonging to nine secondary metabolite-biosynthetic gene clusters of Streptomyces coelicolor A3(2). Investigations on the effect of several metal ions indicated that Cu 2+ , Mn 2+ , and Fe 2+ stimulated AK-111-81 biosynthesis by Streptomyces hygroscopicus, depending on their concentration (Gesheva et al, 2005). Divalent ions stimulated the production of polyenes (Georgieva-Borisova, 1974;Liu et al, 1975;Solivery et al, 1988;Park et al, 1998), and Fe 2+ and Mn 2+ have been found to favor niphimycin production.…”

Section: Introductionmentioning

confidence: 99%

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Zada¹,

Naseem²,

Lee³

et al. 2016

JCKS

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Bacterial strains having the ability to inhibit the growth of other bacteria were isolated from soil samples collected from Kashmir Smast (smast is Pushto for cave), Khyber Pakhtunkhwa, Pakistan. The study includes mineralogical and geochemical analyses of soil sample collected from the cave, so as to describe the habitat from which the microorganisms have been isolated. Total bacterial count of the soil sample was 5.25 3 10 4 CFU mL −1. Four bacterial isolates having activity against test organisms Micrococcus luteus, Klebsiella sp., Pseudomonas sp., and Staphylococcus aureus were screened out for further study. Two of the isolates were found to be Gram-positive and the other two Gram-negative. The four isolates showing antibacterial activity were identified as Serratia sp. KC1-MRL, Bacillus licheniformis KC2-MRL, Bacillus sp. KC3-MRL, and Stenotrophomonas sp. KC4-MRL on the basis of 16S rRNA sequence analysis. Although all isolates showed antibacterial activity, only Bacillus licheniformis KC2-MRL was selected for further study due to its large zone of inhibition. Antibacterial activity of B. licheniformis KC2-MRL was optimum when grown in nutrient broth adjusted to pH 5 and after 24 hours of incubation at 35 uC. The extracted antibacterial compound was stable at pH 5-7 and 40 uC when incubated for 1 hour. The strain was found resistant against cefotaxime (ctx). Atomic-absorption analysis of the soil sample collected from the cave showed high concentrations of calcium (332.938 mg kg ) compared to the control soil collected outside the cave. FTIR spectrum of the concentrated protein showed similarity to bacitracin. The antibacterial compound showed activity against both Gram-negative and positive test strains. Mineralogy of Kashmir Smast is diverse and noteworthy. Different geochemical classes identified by X-ray diffraction were nitrates, oxides, phosphates, silicates, and sulfates. Weathered cave limestone contributes notably to the formation of these minerals or compounds. FTIR spectroscopic analysis helped to identify minerals such as quartz, clinochlore, vermiculite, illite, calcite, and biotite.

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“…The antibacterial metabolite was produced from Streptomyces sp. LH9 using the sterile fermentation broth as a basal medium, which composed from (g/100 ml distilled water): 2.5% glucose, 0.5% soybean, 0.5% NaCl, 2% K 2 HPO 4 , 0.05% MgSO 4 , and 0.01% CaCO 3 at pH 7 [17].…”

Section: Microorganisms Used In the Studymentioning

confidence: 99%

Optimization of Medium Composition for Antibacterial Metabolite Production From Streptomyces Sp.

Al-Ghazali

Omran

2017

Asian J Pharm Clin Res

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Objectives: This paper aimed to optimize some essential nutritional components (carbon, nitrogen, and phosphate) of fermentation medium necessary for the production of antibacterial metabolites from Streptomyces sp. Materials and Methods:Streptomyces sp. LH9 previously isolated from desert soil in Karbala Province, Iraq. This strain produced antibiotic against 4 pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, Streptococcus agalagtiae, and Pseudomonas aeruginosa. For optimizing, the essential nutritional requirements such as carbon, nitrogen, and phosphate in fermentation media different concentrations of these sources were used to improve the antibacterial metabolite production.Results: All the studied nutritional parameters were had impacts on the antibacterial metabolite production from Streptomyces sp. LH9. The actinobacterial strain produced a highest antibiotic metabolites when was grown in the fermentation medium supplemented with 2% dextrose (as a sole carbon source), 0.05% peptone (as a sole nitrogen source), and 0.05% K 2 HPO 4 at pH 7 and incubated under optimal conditions; at 30°C with 250 rpm (revolutions/min) agitation for 7 days.Conclusion: Streptomyces sp. LH9 was a good producer for antibacterial against Gram-positive and Gram-negative bacteria, which required simple nutritional supplements in the fermentation medium. Furthermore, could be utilized the industrial waste for improving the production in the most economic manner.

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Effects of nutrients on the production of AK-111-81 macrolide antibiotic by Streptomyces hygroscopicus

Cited by 78 publications

References 14 publications

Screening, isolation and purification of antibacterial agents from marine actinomycetes

Screening, isolation and purification of antibacterial agents from marine actinomycetes

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Optimization of Medium Composition for Antibacterial Metabolite Production From Streptomyces Sp.

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