Effects of nutrients on biofilm formation and detachment of a Pseudomonas putida strain isolated from a paper machine

Sticky Situations: Key Components That Control Bacterial Surface Attachment

“…For instance, Dewanti and Wong found that Escherichia coli O157:H7 biofilms developed on stainless steel chipped faster and contained a higher number of adherent cells when grown in low-nutrient media than when grown in tryptic soy broth (36). The attachment-upon-starvation response has also been commonly observed for various bacteria, including pathogenic bacteria as well as those found in soil and the marine environment (4,27,65,80,81,97,134). Increased biofilm formation under low-nutrient conditions is not limited to avail-ability of carbon sources, as limitations in nitrogen and phosphorous were also shown to correlate with increased biofilm formation.…”

Biofilm Dispersion

“…Additionally, these investigations showed that regardless of the carbon source, biofilms that were developed in minimal salts medium produced a thicker extracellular matrix, compared to biofilms grown in rich medium, with glucose as the best substrate for biofilm formation. Rochex and Lebeault (2007) investigated the release of cells from a Pseudomonas putida biofilm in a laminar flow cell reactor in defined mineral medium under various nutrient loading conditions. Their investigations showed that increasing the glucose concentration in the reactor from 0.1 to 1.0 gram per liter resulted in an increase in cells released from the biofilm.…”

Factors Affecting Biofilm Development

“…In environments with high nutrient levels, they are found to be more abundant and thicker (Allison et al, 2000;Rochex and Lebeault, 2007). In environments with high nutrient levels, they are found to be more abundant and thicker (Allison et al, 2000;Rochex and Lebeault, 2007).…”