Effects of Noninhibitory Serpin Maspin on the Actin Cytoskeleton: A Quantitative Image Modeling Approach

Al-Mamun, Mohammad A.; Ravenhill, Lorna; Srisukkham, Worawut; Hossain, Alamgir; Fall, C.; Ellis, Vincent; Bass, Rosemary

doi:10.1017/s1431927616000520

Cited by 3 publications

(7 citation statements)

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“…The method integrated two texture analysis methods: GLH and GLCM to find some important information about the shape and behavior of the ruffling region. This paper differs from the previous model developed by Al-Mamun et al, (2016b) in various ways.…”

Section: Discussionmentioning

confidence: 74%

“…In a recent attempt, Al-Mamun et al, (2016b) showed that an image processing tool can be used to quantify three cellular parts: nuclei, cytoplasm and ruffling regions to observe the changes in shape and behavior. The study considers descriptors including area, perimeter, integrated optical density, fractal dimension and circularity to quantify each cellular part.…”

Section: Discussionmentioning

confidence: 99%

“…First, the current version of the method is compared with the level set method suggested in Li et al, (2010), but no comparison was attempted in Al-Mamun et al, (2016b). But it is needed to mention that level set method performs almost similar to the current method.…”

Section: Discussionmentioning

confidence: 99%

“…This preprocessing step is essential because some image samples had background contrast problem due to uneven illumination and striped pattern problems of the microscope. We used median filtering iteratively with contrast limited adaptive histogram Equalization (CLAHE) until the background problem resolved (Al-Mamun et al, 2016b). Normally, CLAHE is useful when both the background and foreground object are bright at the same time or else both are dark at the same time.…”

Section: Nuclei Segmentation Algorithmmentioning

confidence: 99%

“…To date, few computational attempts have been taken to investigate the fact that maspin resides exogenously and acts as cytoskeleton regulator by reducing cell migration and increasing cell-ECM adhesion (Al-Mamun et al, 2013;2016a). Recently, Al-Mamun et al, (2016b) presented a multidisciplinary quantitative image model where reduction of cell migration phenomena was investigated through both in vitro and computational study. This study measured the nuclei, cytoplasmic and ruffling regions using different quantitative descriptors like area, perimeter, and circularity of the cells, fractal dimension, and integrated optical density.…”

Section: Introductionmentioning

confidence: 99%

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A quantitative image analysis for the cellular cytoskeleton during in vitro tumor growth

Al-Mamun

Srisukkham

Farid

et al. 2018

Expert Systems with Applications

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Nuclei Segmentation Algorithmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A quantitative image analysis for the cellular cytoskeleton during in vitro tumor growth

Al-Mamun

Srisukkham

Farid

et al. 2018

Expert Systems with Applications

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show abstract

PI3K-AKT, JAK2-STAT3 pathways and cell–cell contact regulate maspin subcellular localization

et al. 2021

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Background Maspin (SERPINB5) is a potential tumor suppressor gene with pleiotropic biological activities, including regulation of cell proliferation, death, adhesion, migration and gene expression. Several studies indicate that nuclear localization is essential for maspin tumor suppression activity. We have previously shown that the EGFR activation leads to maspin nuclear localization in MCF-10A cells. The present study investigated which EGFR downstream signaling molecules are involved in maspin nuclear localization and explored a possible role of cell–cell contact in this process. Methods MCF-10A cells were treated with pharmacological inhibitors against EGFR downstream pathways followed by EGF treatment. Maspin subcellular localization was determined by immunofluorescence. Proteomic and interactome analyses were conducted to identify maspin-binding proteins in EGF-treated cells only. To investigate the role of cell–cell contact these cells were either treated with chelating agents or plated on different cell densities. Maspin and E-cadherin subcellular localization was determined by immunofluorescence. Results We found that PI3K-Akt and JAK2-STAT3, but not MAP kinase pathway, regulate EGF-induced maspin nuclear accumulation in MCF-10A cells. We observed that maspin is predominantly nuclear in sparse cell culture, but it is redistributed to the cytoplasm in confluent cells even in the presence of EGF. Proteomic and interactome results suggest a role of maspin on post-transcriptional and translation regulation, protein folding and cell–cell adhesion. Conclusions Maspin nuclear accumulation is determined by an interplay between EGFR (via PI3K-Akt and JAK2-STAT3 pathways) and cell–cell contact.

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Integrating the tumor-suppressive activity of Maspin with p53 in retuning the epithelial homeostasis: A working hypothesis and applicable prospects

Tang

Ling

Jiang³

et al. 2022

Front. Oncol.

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Epithelial malignant transformation and tumorous development were believed to be closely associated with the loss of its microenvironment integrity and homeostasis. The tumor-suppressive molecules Maspin and p53 were demonstrated to play a crucial role in body epithelial and immune homeostasis. Downregulation of Maspin and mutation of p53 were frequently associated with malignant transformation and poor prognosis in various human cancers. In this review, we focused on summarizing the progress of the molecular network of Maspin in studying epithelial tumorous development and its response to clinic treatment and try to clarify the underlying antitumor mechanism. Notably, Maspin expression was reported to be transcriptionally activated by p53, and the transcriptional activity of p53 was demonstrated to be enhanced by its acetylation through inhibition of HDAC1. As an endogenous inhibitor of HDAC1, Maspin possibly potentiates the transcriptional activity of p53 by acetylating the p53 protein. Hereby, it could form a “self-propelling” antitumor mechanism. Thus, we summarized that, upon stimulation of cellular stress and by integrating with p53, the aroused Maspin played the epigenetic surveillant role to prevent the epithelial digressional process and retune the epithelial homeostasis, which is involved in activating host immune surveillance, regulating the inflammatory factors, and fine-tuning its associated cell signaling pathways. Consequentially, in a normal physiological condition, activation of the above “self-propelling” antitumor mechanism of Maspin and p53 could reduce cellular stress (e.g., chronic infection/inflammation, oxidative stress, transformation) effectively and achieve cancer prevention. Meanwhile, designing a strategy of mimicking Maspin’s epigenetic regulation activity with integrating p53 tumor-suppressive activity could enhance the chemotherapy efficacy theoretically in a pathological condition of cancer.

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Effects of Noninhibitory Serpin Maspin on the Actin Cytoskeleton: A Quantitative Image Modeling Approach

Abstract: Image analysis results showed that the effects of maspin were mirrored by effects on cell architecture, in a way that could be described quantitatively.

Cited by 3 publications

References 50 publications

A quantitative image analysis for the cellular cytoskeleton during in vitro tumor growth

A quantitative image analysis for the cellular cytoskeleton during in vitro tumor growth

PI3K-AKT, JAK2-STAT3 pathways and cell–cell contact regulate maspin subcellular localization

Integrating the tumor-suppressive activity of Maspin with p53 in retuning the epithelial homeostasis: A working hypothesis and applicable prospects

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